ID   HBOH_BURTA              Reviewed;         698 AA.
AC   Q2SYN3;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=D-(-)-3-hydroxybutyrate oligomer hydrolase {ECO:0000255|HAMAP-Rule:MF_01906};
DE            Short=3HB-oligomer hydrolase {ECO:0000255|HAMAP-Rule:MF_01906};
DE            Short=3HBOH {ECO:0000255|HAMAP-Rule:MF_01906};
DE            EC=3.1.1.22 {ECO:0000255|HAMAP-Rule:MF_01906};
DE   Flags: Precursor;
GN   OrderedLocusNames=BTH_I1420;
OS   Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 /
OS   E264).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; pseudomallei group.
OX   NCBI_TaxID=271848;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700388 / DSM 13276 / CIP 106301 / E264;
RX   PubMed=16336651; DOI=10.1186/1471-2164-6-174;
RA   Kim H.S., Schell M.A., Yu Y., Ulrich R.L., Sarria S.H., Nierman W.C.,
RA   DeShazer D.;
RT   "Bacterial genome adaptation to niches: divergence of the potential
RT   virulence genes in three Burkholderia species of different survival
RT   strategies.";
RL   BMC Genomics 6:174-174(2005).
CC   -!- FUNCTION: Participates in the degradation of poly-3-hydroxybutyrate
CC       (PHB). It works downstream of poly(3-hydroxybutyrate) depolymerase,
CC       hydrolyzing D(-)-3-hydroxybutyrate oligomers of various length (3HB-
CC       oligomers) into 3HB-monomers. {ECO:0000255|HAMAP-Rule:MF_01906}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxybutanoate dimer + H2O = 2 (R)-3-hydroxybutanoate +
CC         H(+); Xref=Rhea:RHEA:10172, ChEBI:CHEBI:10979, ChEBI:CHEBI:10983,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378; EC=3.1.1.22;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01906};
CC   -!- PATHWAY: Lipid metabolism; butanoate metabolism. {ECO:0000255|HAMAP-
CC       Rule:MF_01906}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000255|HAMAP-Rule:MF_01906}.
CC   -!- SIMILARITY: Belongs to the D-(-)-3-hydroxybutyrate oligomer hydrolase
CC       family. {ECO:0000255|HAMAP-Rule:MF_01906}.
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DR   EMBL; CP000086; ABC36292.1; -; Genomic_DNA.
DR   RefSeq; WP_009889457.1; NZ_CP008785.1.
DR   AlphaFoldDB; Q2SYN3; -.
DR   PRIDE; Q2SYN3; -.
DR   EnsemblBacteria; ABC36292; ABC36292; BTH_I1420.
DR   KEGG; bte:BTH_I1420; -.
DR   HOGENOM; CLU_420258_0_0_4; -.
DR   OMA; NPEKDWG; -.
DR   OrthoDB; 865015at2; -.
DR   UniPathway; UPA00863; -.
DR   Proteomes; UP000001930; Chromosome I.
DR   GO; GO:0005615; C:extracellular space; IEA:InterPro.
DR   GO; GO:0047989; F:hydroxybutyrate-dimer hydrolase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019605; P:butyrate metabolic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01906; 3HBOH; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR016582; OHBut_olig_hydro_put.
DR   Pfam; PF10605; 3HBOH; 1.
DR   PIRSF; PIRSF011409; HObutyrate_olig_hydrol; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Secreted; Signal.
FT   SIGNAL          1..32
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01906"
FT   CHAIN           33..698
FT                   /note="D-(-)-3-hydroxybutyrate oligomer hydrolase"
FT                   /id="PRO_0000314426"
FT   ACT_SITE        310
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01906"
SQ   SEQUENCE   698 AA;  71668 MW;  FFF16DEE5155150B CRC64;
     MTTIRGGSRR ASLPALALLG VLLGACHSDD NAQVNTLPGF VSGNVRKTAY DGASDDLLTA
     GLGKTGLGSD TRPGFANPAQ PTAAELRRLA IYSNYRALVD ITPNGGYGRF WGPNVDLAGN
     DTLGEGKIAG TEYLAYSDDG SGRKNVTLLV QVPASFDPAN PCIVTATSSG SRGVYGAIAA
     AGEWGLKRGC AVAYNDKGGG NGAHEIGTGV VTLIDGTLAS ASSAGSASLF TASESSSALA
     AFNSAFPNRY AYKHAHSQQN PEQDWGRVTL QAVEFAYWAL NEQFGPAIDG ARHGVRYRPG
     DITTIAASVS NGGAAALAAA EQDTRGWITA VVVGEPQINV RMTPGVTVEQ GGVPAPSFGR
     PLADYATLAN LLQPCAAAAV AAVGAPYLSA LPVGLTQSIR VQRCATLAAA GLVSGADTAS
     QANDALAQLH AAGYLADSDL LQAPMWDSQA IPAIAVTYAN AYTRSRVTDN LCNFSFATTN
     SVTGAVAPPA TSPMTSLFGA GNGVPPTNGI NLVFNGASGG VDHRLATPDA SFAGAFCLRQ
     LWTANQLGIG ANVDAVRVAA NLQRKPAIIV QGRSDALVPV NHASRAYVAQ NSATEGRASQ
     LSFYEVTNGQ HFDAFLSVPG FDTRFVPVHY YDEQALNLMW NHLKSGAPLP PSQVIRTVPR
     GGVPGAAPAL STANLPPIVQ SPGSNAITVN AGVIDVPL