ID   HBOH_BURVG              Reviewed;         696 AA.
AC   A4JBY8;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2007, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=D-(-)-3-hydroxybutyrate oligomer hydrolase {ECO:0000255|HAMAP-Rule:MF_01906};
DE            Short=3HB-oligomer hydrolase {ECO:0000255|HAMAP-Rule:MF_01906};
DE            Short=3HBOH {ECO:0000255|HAMAP-Rule:MF_01906};
DE            EC=3.1.1.22 {ECO:0000255|HAMAP-Rule:MF_01906};
DE   Flags: Precursor;
GN   OrderedLocusNames=Bcep1808_0779;
OS   Burkholderia vietnamiensis (strain G4 / LMG 22486) (Burkholderia cepacia
OS   (strain R1808)).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX   NCBI_TaxID=269482;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=G4 / LMG 22486;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Tiedje J., Richardson P.;
RT   "Complete sequence of chromosome 1 of Burkholderia vietnamiensis G4.";
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Participates in the degradation of poly-3-hydroxybutyrate
CC       (PHB). It works downstream of poly(3-hydroxybutyrate) depolymerase,
CC       hydrolyzing D(-)-3-hydroxybutyrate oligomers of various length (3HB-
CC       oligomers) into 3HB-monomers. {ECO:0000255|HAMAP-Rule:MF_01906}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxybutanoate dimer + H2O = 2 (R)-3-hydroxybutanoate +
CC         H(+); Xref=Rhea:RHEA:10172, ChEBI:CHEBI:10979, ChEBI:CHEBI:10983,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378; EC=3.1.1.22;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01906};
CC   -!- PATHWAY: Lipid metabolism; butanoate metabolism. {ECO:0000255|HAMAP-
CC       Rule:MF_01906}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000255|HAMAP-Rule:MF_01906}.
CC   -!- SIMILARITY: Belongs to the D-(-)-3-hydroxybutyrate oligomer hydrolase
CC       family. {ECO:0000255|HAMAP-Rule:MF_01906}.
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DR   EMBL; CP000614; ABO53791.1; -; Genomic_DNA.
DR   AlphaFoldDB; A4JBY8; -.
DR   STRING; 269482.Bcep1808_0779; -.
DR   ESTHER; burvg-hboh; OHBut_olig_hydro_put.
DR   EnsemblBacteria; ABO53791; ABO53791; Bcep1808_0779.
DR   KEGG; bvi:Bcep1808_0779; -.
DR   eggNOG; ENOG502Z8QU; Bacteria.
DR   HOGENOM; CLU_420258_0_0_4; -.
DR   OMA; NPEKDWG; -.
DR   UniPathway; UPA00863; -.
DR   Proteomes; UP000002287; Chromosome 1.
DR   GO; GO:0005615; C:extracellular space; IEA:InterPro.
DR   GO; GO:0047989; F:hydroxybutyrate-dimer hydrolase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019605; P:butyrate metabolic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01906; 3HBOH; 1.
DR   InterPro; IPR016582; OHBut_olig_hydro_put.
DR   Pfam; PF10605; 3HBOH; 1.
DR   PIRSF; PIRSF011409; HObutyrate_olig_hydrol; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01906"
FT   CHAIN           27..696
FT                   /note="D-(-)-3-hydroxybutyrate oligomer hydrolase"
FT                   /id="PRO_5000231743"
FT   ACT_SITE        309
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01906"
SQ   SEQUENCE   696 AA;  71688 MW;  9824C126919E25AA CRC64;
     MDTHGWGSRI LVGAALAALT MLGACNGDET AERNQLPVFV SGSVRTTAYD GASDDLLTAG
     LGKTGLASAA APAFADPSRP TAAELRRVAI WSNYRALVDM SANGGYGRFW GPNVDLDGND
     TLGEGKIAGT EYLAYADDGS GRKNVTLLVQ VPASFDPAQP CIVTATSSGS RGVYGAISAA
     GEWGLKRGCA VAYNDKGGGN GAQELGSNTV TLIDGTLANA VLAGSASLFT ANLTSGDLAA
     FNSRFPNRYA FKHAHSQQNP EHDWGRATLQ SVEFAYWALN QQFAPLVDGS HRGVRYRAGD
     ITTIAASVSN GGGAALAAAE QDTRRWITAV VVGEPQINVR MAPNAVVRES GRPVPSFGRP
     LADYATLANL LEPCAAASAS LAGEPYLSAL PLATTQSIRT QRCATLAAAG LVSGADTQSQ
     AADALAQLHA AGYLADSDLL QASMWDSQAI PAIAVTYANA YTRSSVADNL CNFSFATTNA
     ATGAVAAPAA SPMPAVFGLG NGVPPTAGIN LVFNTGAGVD HRLATPDASF AGALCLRQLW
     TNGMLDMPAN VEAVRVNANL QGKPAIIVQG RSDALVPVNH ASRAYVAQNS ISEGGRSQLV
     FYEVTNGQHF DAFLPVAGFD TRFVPVHYYN VQALNLMWRH LKSGAPLPPS QVIRTVPRGG
     TPGAAPALTS ANLPPISTAP GANAIAAGAG AIDVPL