ID   HBOH_POLNA              Reviewed;         706 AA.
AC   A1VUF3;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-JAN-2008, sequence version 2.
DT   25-MAY-2022, entry version 63.
DE   RecName: Full=D-(-)-3-hydroxybutyrate oligomer hydrolase {ECO:0000255|HAMAP-Rule:MF_01906};
DE            Short=3HB-oligomer hydrolase {ECO:0000255|HAMAP-Rule:MF_01906};
DE            Short=3HBOH {ECO:0000255|HAMAP-Rule:MF_01906};
DE            EC=3.1.1.22 {ECO:0000255|HAMAP-Rule:MF_01906};
DE   Flags: Precursor;
GN   OrderedLocusNames=Pnap_3987;
OS   Polaromonas naphthalenivorans (strain CJ2).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Polaromonas.
OX   NCBI_TaxID=365044;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CJ2;
RX   PubMed=19453698; DOI=10.1111/j.1462-2920.2009.01947.x;
RA   Yagi J.M., Sims D., Brettin T., Bruce D., Madsen E.L.;
RT   "The genome of Polaromonas naphthalenivorans strain CJ2, isolated from coal
RT   tar-contaminated sediment, reveals physiological and metabolic versatility
RT   and evolution through extensive horizontal gene transfer.";
RL   Environ. Microbiol. 11:2253-2270(2009).
CC   -!- FUNCTION: Participates in the degradation of poly-3-hydroxybutyrate
CC       (PHB). It works downstream of poly(3-hydroxybutyrate) depolymerase,
CC       hydrolyzing D(-)-3-hydroxybutyrate oligomers of various length (3HB-
CC       oligomers) into 3HB-monomers. {ECO:0000255|HAMAP-Rule:MF_01906}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxybutanoate dimer + H2O = 2 (R)-3-hydroxybutanoate +
CC         H(+); Xref=Rhea:RHEA:10172, ChEBI:CHEBI:10979, ChEBI:CHEBI:10983,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378; EC=3.1.1.22;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01906};
CC   -!- PATHWAY: Lipid metabolism; butanoate metabolism. {ECO:0000255|HAMAP-
CC       Rule:MF_01906}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000255|HAMAP-Rule:MF_01906}.
CC   -!- SIMILARITY: Belongs to the D-(-)-3-hydroxybutyrate oligomer hydrolase
CC       family. {ECO:0000255|HAMAP-Rule:MF_01906}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABM39281.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CP000529; ABM39281.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_041376840.1; NC_008781.1.
DR   AlphaFoldDB; A1VUF3; -.
DR   STRING; 365044.Pnap_3987; -.
DR   ESTHER; polna-hboh; OHBut_olig_hydro_put.
DR   PRIDE; A1VUF3; -.
DR   EnsemblBacteria; ABM39281; ABM39281; Pnap_3987.
DR   KEGG; pna:Pnap_3987; -.
DR   eggNOG; ENOG502Z8QU; Bacteria.
DR   HOGENOM; CLU_420258_0_0_4; -.
DR   OrthoDB; 865015at2; -.
DR   UniPathway; UPA00863; -.
DR   Proteomes; UP000000644; Chromosome.
DR   GO; GO:0005615; C:extracellular space; IEA:InterPro.
DR   GO; GO:0047989; F:hydroxybutyrate-dimer hydrolase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019605; P:butyrate metabolic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01906; 3HBOH; 1.
DR   InterPro; IPR016582; OHBut_olig_hydro_put.
DR   Pfam; PF10605; 3HBOH; 1.
DR   PIRSF; PIRSF011409; HObutyrate_olig_hydrol; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01906"
FT   CHAIN           28..706
FT                   /note="D-(-)-3-hydroxybutyrate oligomer hydrolase"
FT                   /id="PRO_0000314428"
FT   ACT_SITE        311
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01906"
SQ   SEQUENCE   706 AA;  72290 MW;  8696D73AEB13396B CRC64;
     MTIIIAGKNT LTLTSLAAAV LALGACGGNS DPFESKNTKP AYLGAVAIAS YDGASDDLLT
     AGLGKTGLGG TAPAVADPLK PTPAELRRLA IFNNYRAILD ISTNGGYGTL YGPNVDAKGV
     ITTGEGKIAG TEYIAYSDDG TGRQNITMMV QVPASFNPAN ACIVTGTSSG SRGVYGAIGS
     AGEWGLKNGC AVAYTDKGTG TGIHDLQNNT VNVQNGVRTD AAAAGKNSIF TAELSASERA
     AFNAATPNRF AVKHAHSQQN PEKDWGKWTL QSVEFAYFVL NEKYGDLARD GATHLKKLTP
     SNTIVIASSV SNGAGAALAA AEQDTQGLIS GVAVAEPEVQ LAPDARLSVK RGASVLVGTG
     KPLYDYFTLA NLLQPCAALV SPATNAFNTV NAATATNRCS ALKANGLVTG TTTAEQAASA
     LAALVAAGWQ PESNVLQASH YSFATLSVGL TYANTYGRFS VKDNLCGFSF AATGAAASAT
     PNAPVPASAS ALATSFGASN GVPPTIGINI VNNLSAGGPL LDAASLSAGG VQDYNIAGAL
     CMRELATGSS ANAVRVRQGM SEVVRSANLR GKPALIVQGR ADTLLPVAFT GRPYYGMNKI
     VEGTASRLSY IEVTNAQHFD AFLAFPGYPE RMVPLHRYFI QAMDMMYANL KTGAALPASQ
     VVRTVPRGLT GAVANPIAAS NVPPIKTTPA AADQITFANN VVTIAD