ID   HBOH_POLSJ              Reviewed;         706 AA.
AC   Q122D1;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   22-AUG-2006, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=D-(-)-3-hydroxybutyrate oligomer hydrolase {ECO:0000255|HAMAP-Rule:MF_01906};
DE            Short=3HB-oligomer hydrolase {ECO:0000255|HAMAP-Rule:MF_01906};
DE            Short=3HBOH {ECO:0000255|HAMAP-Rule:MF_01906};
DE            EC=3.1.1.22 {ECO:0000255|HAMAP-Rule:MF_01906};
DE   Flags: Precursor;
GN   OrderedLocusNames=Bpro_4732;
OS   Polaromonas sp. (strain JS666 / ATCC BAA-500).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Polaromonas; unclassified Polaromonas.
OX   NCBI_TaxID=296591;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JS666 / ATCC BAA-500;
RX   PubMed=18723656; DOI=10.1128/aem.00197-08;
RA   Mattes T.E., Alexander A.K., Richardson P.M., Munk A.C., Han C.S.,
RA   Stothard P., Coleman N.V.;
RT   "The genome of Polaromonas sp. strain JS666: insights into the evolution of
RT   a hydrocarbon- and xenobiotic-degrading bacterium, and features of
RT   relevance to biotechnology.";
RL   Appl. Environ. Microbiol. 74:6405-6416(2008).
CC   -!- FUNCTION: Participates in the degradation of poly-3-hydroxybutyrate
CC       (PHB). It works downstream of poly(3-hydroxybutyrate) depolymerase,
CC       hydrolyzing D(-)-3-hydroxybutyrate oligomers of various length (3HB-
CC       oligomers) into 3HB-monomers. {ECO:0000255|HAMAP-Rule:MF_01906}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxybutanoate dimer + H2O = 2 (R)-3-hydroxybutanoate +
CC         H(+); Xref=Rhea:RHEA:10172, ChEBI:CHEBI:10979, ChEBI:CHEBI:10983,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378; EC=3.1.1.22;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01906};
CC   -!- PATHWAY: Lipid metabolism; butanoate metabolism. {ECO:0000255|HAMAP-
CC       Rule:MF_01906}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000255|HAMAP-Rule:MF_01906}.
CC   -!- SIMILARITY: Belongs to the D-(-)-3-hydroxybutyrate oligomer hydrolase
CC       family. {ECO:0000255|HAMAP-Rule:MF_01906}.
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DR   EMBL; CP000316; ABE46611.1; -; Genomic_DNA.
DR   RefSeq; WP_011485596.1; NC_007948.1.
DR   AlphaFoldDB; Q122D1; -.
DR   STRING; 296591.Bpro_4732; -.
DR   ESTHER; polsj-hboh; OHBut_olig_hydro_put.
DR   EnsemblBacteria; ABE46611; ABE46611; Bpro_4732.
DR   KEGG; pol:Bpro_4732; -.
DR   eggNOG; ENOG502Z8QU; Bacteria.
DR   HOGENOM; CLU_420258_0_0_4; -.
DR   OMA; NPEKDWG; -.
DR   OrthoDB; 865015at2; -.
DR   UniPathway; UPA00863; -.
DR   Proteomes; UP000001983; Chromosome.
DR   GO; GO:0005615; C:extracellular space; IEA:InterPro.
DR   GO; GO:0047989; F:hydroxybutyrate-dimer hydrolase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019605; P:butyrate metabolic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01906; 3HBOH; 1.
DR   InterPro; IPR016582; OHBut_olig_hydro_put.
DR   Pfam; PF10605; 3HBOH; 1.
DR   PIRSF; PIRSF011409; HObutyrate_olig_hydrol; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..32
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01906"
FT   CHAIN           33..706
FT                   /note="D-(-)-3-hydroxybutyrate oligomer hydrolase"
FT                   /id="PRO_5000117312"
FT   ACT_SITE        311
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01906"
SQ   SEQUENCE   706 AA;  72212 MW;  92792A8B4905943B CRC64;
     MTTTSKNCLT LTSIAAAVAA VLVLSACGGG SAGENINRKP TYLGTVVSVN YDGASDDLLT
     AGLGKTGLGA TAPVAADPLN PTAAELRRIA IFNNYRALLD ISVAGGYGSL YGPNVDASGV
     ITTSEGKIAG TEYMAYSDDG TGNQNITMLV QVPTTFNPAS PCIVTGTSSG SRGVYGAIGT
     SGEWGLKNGC AVAYTDKGTG TGIHDLQNDT VNLQRGERAT ATAAGKASNF TASLTSTERA
     AFNTATPNRF AVKHAHSQQN TEKDWGKWTL QAVEFAYFVL NENYGDAAKD GVSRLVKLKP
     ANTIVIASSA SNGAGAALAA AELDTKGLIT GVAVAEPQIQ VVPDTRLSVR RGASTLGGTG
     RSLFDYASLG NLLQPCAALA SPTTNVFNTV NTTIATNRCN ALQASGLIVG NTTAELAADA
     MARLLAAGHQ PESSVLQASH YSFATPAVAV TFANSYGRFS VKDNLCGFSF AATGAAGSAT
     PNAPVAASAA ALATSFGASN GIPPTVGINI VNNNSVGGPL LDAASLSAGS VLDYNIAGAL
     CLRELLGGSS ANALKVQQGI NEVLRTGDLQ GKPALIVHGR ADAQVPVAFS SRPYFGRNKI
     VEGANSRLSY IEVTNAQHFD AFLAFPGYGE RFVPAHRYFI QAMDMMYANL KTGAALPASQ
     VVRTVPRGLT GAVVNPITPA NVPPIKTVPA VADQITFANN VVTVAD