ID   HBOH_RALSO              Reviewed;         725 AA.
AC   Q8XZR1;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=D-(-)-3-hydroxybutyrate oligomer hydrolase {ECO:0000255|HAMAP-Rule:MF_01906};
DE            Short=3HB-oligomer hydrolase {ECO:0000255|HAMAP-Rule:MF_01906};
DE            Short=3HBOH {ECO:0000255|HAMAP-Rule:MF_01906};
DE            EC=3.1.1.22 {ECO:0000255|HAMAP-Rule:MF_01906};
DE   Flags: Precursor;
GN   OrderedLocusNames=RSc1334;
OS   Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Ralstonia.
OX   NCBI_TaxID=267608;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GMI1000;
RX   PubMed=11823852; DOI=10.1038/415497a;
RA   Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M.,
RA   Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M.,
RA   Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M.,
RA   Moisan A., Robert C., Saurin W., Schiex T., Siguier P., Thebault P.,
RA   Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A.;
RT   "Genome sequence of the plant pathogen Ralstonia solanacearum.";
RL   Nature 415:497-502(2002).
CC   -!- FUNCTION: Participates in the degradation of poly-3-hydroxybutyrate
CC       (PHB). It works downstream of poly(3-hydroxybutyrate) depolymerase,
CC       hydrolyzing D(-)-3-hydroxybutyrate oligomers of various length (3HB-
CC       oligomers) into 3HB-monomers. {ECO:0000255|HAMAP-Rule:MF_01906}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxybutanoate dimer + H2O = 2 (R)-3-hydroxybutanoate +
CC         H(+); Xref=Rhea:RHEA:10172, ChEBI:CHEBI:10979, ChEBI:CHEBI:10983,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378; EC=3.1.1.22;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01906};
CC   -!- PATHWAY: Lipid metabolism; butanoate metabolism. {ECO:0000255|HAMAP-
CC       Rule:MF_01906}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000255|HAMAP-Rule:MF_01906}.
CC   -!- SIMILARITY: Belongs to the D-(-)-3-hydroxybutyrate oligomer hydrolase
CC       family. {ECO:0000255|HAMAP-Rule:MF_01906}.
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DR   EMBL; AL646052; CAD15036.1; -; Genomic_DNA.
DR   RefSeq; WP_011001283.1; NC_003295.1.
DR   AlphaFoldDB; Q8XZR1; -.
DR   STRING; 267608.RSc1334; -.
DR   ESTHER; ralso-hboh; OHBut_olig_hydro_put.
DR   EnsemblBacteria; CAD15036; CAD15036; RSc1334.
DR   GeneID; 60500856; -.
DR   KEGG; rso:RSc1334; -.
DR   PATRIC; fig|267608.8.peg.1358; -.
DR   eggNOG; ENOG502Z8QU; Bacteria.
DR   HOGENOM; CLU_420258_0_0_4; -.
DR   OMA; NPEKDWG; -.
DR   UniPathway; UPA00863; -.
DR   Proteomes; UP000001436; Chromosome.
DR   GO; GO:0005615; C:extracellular space; IEA:InterPro.
DR   GO; GO:0047989; F:hydroxybutyrate-dimer hydrolase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019605; P:butyrate metabolic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01906; 3HBOH; 1.
DR   InterPro; IPR016582; OHBut_olig_hydro_put.
DR   Pfam; PF10605; 3HBOH; 1.
DR   PIRSF; PIRSF011409; HObutyrate_olig_hydrol; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01906"
FT   CHAIN           23..725
FT                   /note="D-(-)-3-hydroxybutyrate oligomer hydrolase"
FT                   /id="PRO_0000314431"
FT   ACT_SITE        322
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01906"
SQ   SEQUENCE   725 AA;  73528 MW;  78B9A592B5C66293 CRC64;
     MNTTRDANRL RQRASLSGLA LAGSLGLAGC GVLGLAGCGG DNNSSTSGNV KPSFVGTVTV
     TRHDGANDDL LTAGLGAAGL ASASAPSVAT PTAPTAAELR RLDIYASYRA LVDTAANGGY
     GTLYGPSVDV NGNATSGSGM VPGVEYIAYS DDGTGQQNVV LLVQIPDAFD PANPCIITAT
     SSGSRGIYGA ISTGEWGLKH KCAVAYTDKG TGAGPHDLAT DTVPLQDGTR TTRLAAGRTA
     QFAAPLTDSQ LAAFNAATPN RLAFKHAHSQ RNPEKDWGLF TLQAVQFAFW AINDKLGAAG
     MPAGSPLPVR RDNTIVIASS ISNGGGAAIA AAEQDTAGWI DGVAVGEPGL NLPPSANVQV
     ARGGVTLPVS GKPLFDYVSY ANLFRLCAAS SSGVSAAPTQ AFFAGAVGWP ASVQANRCAA
     LRANGLLSSA TAAAQADEAL QKLHAFGWEP ESDLVHASMS YFEIDPSVAV TFGNALARAS
     VLDNLCNFSF AAVDSAFHPT AVNATSLAQM AALGNGIPPT SGVQLINNLA QGGPTQSKQS
     VDGSGALAAN LDGALCLRNL LTGGDAASLA LQAGIAQTLR TGNLHGKPAL IVQGRNDALL
     PVNHGARPYL GLNAQTDSSS RLSYIEVMNA QHFDGFIDLV PGYDTLFVPL VLYEQRALDA
     VFANLKNGTP LPPSQVVRTT PRGGTAGAAP AITAANVPNF TMTPAAGDRI GVSVSGGVAT
     VSVPN