HC173_ARATH
ID HC173_ARATH Reviewed; 598 AA.
AC Q8W4D6;
DT 31-JUL-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 25-MAY-2022, entry version 148.
DE RecName: Full=Protein HIGH CHLOROPHYLL FLUORESCENCE PHENOTYPE 173, chloroplastic {ECO:0000303|PubMed:17435084};
DE Flags: Precursor;
GN Name=HCF173 {ECO:0000303|PubMed:17435084};
GN OrderedLocusNames=At1g16720 {ECO:0000312|Araport:AT1G16720};
GN ORFNames=F19K19.14 {ECO:0000312|EMBL:AEE29489.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, AND SUBUNIT.
RX PubMed=17435084; DOI=10.1105/tpc.106.042895;
RA Schult K., Meierhoff K., Paradies S., Toeller T., Wolff P., Westhoff P.;
RT "The nuclear-encoded factor HCF173 is involved in the initiation of
RT translation of the psbA mRNA in Arabidopsis thaliana.";
RL Plant Cell 19:1329-1346(2007).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, AND SUBUNIT.
RX PubMed=23027666; DOI=10.1104/pp.112.205104;
RA Link S., Engelmann K., Meierhoff K., Westhoff P.;
RT "The atypical short-chain dehydrogenases HCF173 and HCF244 are jointly
RT involved in translational initiation of the psbA mRNA of Arabidopsis.";
RL Plant Physiol. 160:2202-2218(2012).
RN [6]
RP REVIEW ON PHOTOSYSTEM II ASSEMBLY.
RX PubMed=26909098; DOI=10.3389/fpls.2016.00168;
RA Lu Y.;
RT "Identification and roles of photosystem II assembly, stability, and repair
RT factors in Arabidopsis.";
RL Front. Plant Sci. 7:168-168(2016).
RN [7]
RP SUBUNIT.
RC STRAIN=cv. Columbia;
RX PubMed=29438089; DOI=10.1104/pp.17.01782;
RA Myouga F., Takahashi K., Tanaka R., Nagata N., Kiss A.Z., Funk C.,
RA Nomura Y., Nakagami H., Jansson S., Shinozaki K.;
RT "Stable accumulation of photosystem II requires ONE-HELIX PROTEIN1 (OHP1)
RT of the light harvesting-like family.";
RL Plant Physiol. 176:2277-2291(2018).
RN [8]
RP INTERACTION WITH LPE1.
RC STRAIN=cv. Columbia;
RX PubMed=29891689; DOI=10.1073/pnas.1807364115;
RA Jin H., Fu M., Duan Z., Duan S., Li M., Dong X., Liu B., Feng D., Wang J.,
RA Peng L., Wang H.-B.;
RT "LOW PHOTOSYNTHETIC EFFICIENCY 1 is required for light-regulated
RT photosystem II biogenesis in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 115:E6075-E6084(2018).
RN [9]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=30844105; DOI=10.1111/tpj.14308;
RA Williams-Carrier R., Brewster C., Belcher S.E., Rojas M.,
RA Chotewutmontri P., Ljungdahl S., Barkan A.;
RT "The Arabidopsis pentatricopeptide repeat protein LPE1 and its maize
RT ortholog are required for translation of the chloroplast psbJ RNA.";
RL Plant J. 99:56-66(2019).
CC -!- FUNCTION: Auxiliary factor required, together with HCF244, for the
CC biogenesis of photosystem II (PSII), especially for the synthesis of
CC the reaction center proteins (e.g. D1), via the regulation of the
CC corresponding mRNA (e.g. psbA) translation initiation (ribosomal
CC loading) and stabilization. {ECO:0000269|PubMed:17435084,
CC ECO:0000269|PubMed:30844105}.
CC -!- SUBUNIT: Component of a high molecular weight complex containing psbA
CC mRNA, OHP1, OHP2 and HCF244, and PSII core proteins D1/D2, HCF136 and
CC HCF173 (PubMed:17435084, PubMed:23027666, PubMed:29438089). Interacts
CC with LPE1 (PubMed:29891689). {ECO:0000269|PubMed:17435084,
CC ECO:0000269|PubMed:23027666, ECO:0000269|PubMed:29438089,
CC ECO:0000269|PubMed:29891689}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane
CC {ECO:0000269|PubMed:17435084}. Plastid, chloroplast thylakoid membrane
CC {ECO:0000269|PubMed:23027666}; Peripheral membrane protein
CC {ECO:0000269|PubMed:23027666}; Stromal side
CC {ECO:0000269|PubMed:23027666}. Plastid, chloroplast stroma
CC {ECO:0000269|PubMed:17435084}. Note=Predominantly present at thylakoid
CC membranes. {ECO:0000269|PubMed:23027666}.
CC -!- DISRUPTION PHENOTYPE: Impaired photoautotrophy (PubMed:17435084,
CC PubMed:23027666). Seedling lethal (PubMed:23027666). High chlorophyll
CC fluorescence phenotype and severely affected photosystem II (PSII)
CC subunits accumulation associated with a drastically decreased synthesis
CC of the reaction center protein D1 due to a reduced translation
CC initiation (ribosomal loading) of the corresponding psbA mRNA
CC (PubMed:17435084, PubMed:23027666, PubMed:30844105). Plants lacking
CC both HCF173 and HCF244 display stronger PSII defects (PubMed:23027666).
CC {ECO:0000269|PubMed:17435084, ECO:0000269|PubMed:23027666,
CC ECO:0000269|PubMed:30844105}.
CC -!- SIMILARITY: Belongs to the NmrA-type oxidoreductase family.
CC {ECO:0000305}.
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DR EMBL; AC011808; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002684; AEE29489.1; -; Genomic_DNA.
DR EMBL; AY062632; AAL32710.1; -; mRNA.
DR EMBL; AY128723; AAM91123.1; -; mRNA.
DR RefSeq; NP_173116.1; NM_101533.4.
DR AlphaFoldDB; Q8W4D6; -.
DR SMR; Q8W4D6; -.
DR IntAct; Q8W4D6; 1.
DR STRING; 3702.AT1G16720.1; -.
DR iPTMnet; Q8W4D6; -.
DR PaxDb; Q8W4D6; -.
DR PRIDE; Q8W4D6; -.
DR EnsemblPlants; AT1G16720.1; AT1G16720.1; AT1G16720.
DR GeneID; 838243; -.
DR Gramene; AT1G16720.1; AT1G16720.1; AT1G16720.
DR KEGG; ath:AT1G16720; -.
DR Araport; AT1G16720; -.
DR TAIR; locus:2015651; AT1G16720.
DR eggNOG; KOG1203; Eukaryota.
DR HOGENOM; CLU_029746_0_0_1; -.
DR InParanoid; Q8W4D6; -.
DR OMA; YEYVAEP; -.
DR PhylomeDB; Q8W4D6; -.
DR PRO; PR:Q8W4D6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8W4D6; baseline and differential.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR GO; GO:0031969; C:chloroplast membrane; IDA:UniProtKB.
DR GO; GO:0009570; C:chloroplast stroma; IDA:UniProtKB.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009523; C:photosystem II; IEA:UniProtKB-KW.
DR GO; GO:0042651; C:thylakoid membrane; IDA:UniProtKB.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0003723; F:RNA binding; IPI:TAIR.
DR GO; GO:0010207; P:photosystem II assembly; IMP:UniProtKB.
DR GO; GO:0006413; P:translational initiation; IMP:UniProtKB.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR013857; NADH-UbQ_OxRdtase-assoc_prot30.
DR Pfam; PF08547; CIA30; 1.
DR Pfam; PF13460; NAD_binding_10; 2.
DR SUPFAM; SSF49785; SSF49785; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Membrane; Photosynthesis; Photosystem II; Plastid;
KW Reference proteome; Thylakoid; Transit peptide.
FT TRANSIT 1..79
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 80..598
FT /note="Protein HIGH CHLOROPHYLL FLUORESCENCE PHENOTYPE 173,
FT chloroplastic"
FT /id="PRO_0000447637"
FT REGION 42..106
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 42..58
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 59..81
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 598 AA; 65709 MW; EDCEBF6A078498D1 CRC64;
MVGSIVGSNM AATDARFLSS NFGNSFSINT RIHRFHDRSQ IVIPRAQSSS SPSPSPPSDK
KKTKTRPGTI TTKESEETVA KKLDVAPPSP QSPPSPPTLK LDDVNPVGLG RRSRQIFDEV
WRKFSGLGQM SRTTRPDEQE TLDSLLIREG PMCEFAVPGA QNVTVLVVGA TSRIGRIVVR
KLMLRGYTVK ALVRKQDEEV MSMLPRSVDI VVGDVGEPST LKSAVESCSK IIYCATARST
ITADLTRVDH LGVYNLTKAF QDYNNRLAQL RAGKSSKSKL LLAKFKSAES LDGWEIRQGT
YFQDTTASKY DGGMDAKFEF TETERAEFSG YVFTRGGYVE LSKKLSLPLG TTLDRYEGLV
LSVGGNGRSY VVILEAGPSS DMSQSKQYFA RISTKAGFCR VRVPFSAFRP VNPEDPPLDP
FLVHTLTIRF EPKRQRPVDG LAGAQQDLRS FSLVFEYIKA LPAGQETDFI LVSCTGSGVE
ANRREQVLKA KRAGEDSLRR SGLGYTIIRP GPLKEEPGGQ RALIFDQGNR ISQGISCADV
ADICVKALHD STARNKSFDV CHEYVAEQGI ELYELVAHLP DKANNYLTPA LSVLEKNT
