HC244_ARATH
ID HC244_ARATH Reviewed; 395 AA.
AC O65502;
DT 31-JUL-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 25-MAY-2022, entry version 148.
DE RecName: Full=Protein HIGH CHLOROPHYLL FLUORESCENCE PHENOTYPE 244, chloroplastic {ECO:0000303|PubMed:23027666};
DE Flags: Precursor;
GN Name=HCF244 {ECO:0000303|PubMed:23027666};
GN OrderedLocusNames=At4g35250 {ECO:0000312|Araport:AT4G35250};
GN ORFNames=F23E12.190 {ECO:0000312|EMBL:CAA18744.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, AND SUBUNIT.
RX PubMed=23027666; DOI=10.1104/pp.112.205104;
RA Link S., Engelmann K., Meierhoff K., Westhoff P.;
RT "The atypical short-chain dehydrogenases HCF173 and HCF244 are jointly
RT involved in translational initiation of the psbA mRNA of Arabidopsis.";
RL Plant Physiol. 160:2202-2218(2012).
RN [6]
RP REVIEW ON PHOTOSYSTEM II ASSEMBLY.
RX PubMed=26909098; DOI=10.3389/fpls.2016.00168;
RA Lu Y.;
RT "Identification and roles of photosystem II assembly, stability, and repair
RT factors in Arabidopsis.";
RL Front. Plant Sci. 7:168-168(2016).
RN [7]
RP SUBUNIT, AND INTERACTION WITH OHP1.
RC STRAIN=cv. Columbia;
RX PubMed=29438089; DOI=10.1104/pp.17.01782;
RA Myouga F., Takahashi K., Tanaka R., Nagata N., Kiss A.Z., Funk C.,
RA Nomura Y., Nakagami H., Jansson S., Shinozaki K.;
RT "Stable accumulation of photosystem II requires ONE-HELIX PROTEIN1 (OHP1)
RT of the light harvesting-like family.";
RL Plant Physiol. 176:2277-2291(2018).
RN [8]
RP FUNCTION, AND INTERACTION WITH OHP1 AND OHP2.
RX PubMed=29930106; DOI=10.1104/pp.18.00540;
RA Hey D., Grimm B.;
RT "ONE-HELIX PROTEIN2 (OHP2) is required for the stability of OHP1 and
RT assembly factor HCF244 and is functionally linked to PSII biogenesis.";
RL Plant Physiol. 177:1453-1472(2018).
RN [9]
RP FUNCTION, AND INTERACTION WITH OHP1 AND OHP2.
RX PubMed=30397023; DOI=10.1104/pp.18.01231;
RA Li Y., Liu B., Zhang J., Kong F., Zhang L., Meng H., Li W., Rochaix J.D.,
RA Li D., Peng L.;
RT "OHP1, OHP2, and HCF244 form a transient functional complex with the
RT photosystem II reaction center.";
RL Plant Physiol. 179:195-208(2019).
RN [10]
RP FUNCTION.
RX PubMed=31991763; DOI=10.3390/plants9020152;
RA Chotewutmontri P., Williams-Carrier R., Barkan A.;
RT "Exploring the Link between Photosystem II Assembly and Translation of the
RT Chloroplast psbA mRNA.";
RL Plants (Basel) 9:0-0(2020).
CC -!- FUNCTION: Auxiliary factor required, together with HCF173, for the
CC biogenesis of photosystem II (PSII), especially for the synthesis of
CC the reaction center proteins (e.g. D1), via the regulation of the
CC corresponding mRNA (e.g. psbA) translation initiation (ribosomal
CC loading) and stabilization (PubMed:29930106). Forms a trimeric complex
CC with OHP1 and OHP2 that is required to promote PSII core subunit
CC assembly (PubMed:29930106, PubMed:30397023). The trimeric complex forms
CC a transient PSII reaction center-like complex with PsbA, PsbD, PsbE,
CC PsbF and PsbI subunits in thylakoids for early assembly of PSII as well
CC as PSII repair (PubMed:30397023). The trimeric complex is required for
CC the recruitment of ribosomes to the psbA mRNA during PSII biogenesis
CC and repair (PubMed:31991763). {ECO:0000269|PubMed:29930106,
CC ECO:0000269|PubMed:30397023, ECO:0000269|PubMed:31991763}.
CC -!- SUBUNIT: Component of a high molecular weight complex containing OHP1,
CC OHP2 and HCF244, and PSII core proteins D1/D2, HCF136 and HCF173
CC (PubMed:23027666, PubMed:29438089). Interacts with OHP1
CC (PubMed:29438089). Forms a trimeric complex with OHP1 and OHP2 that
CC mutually stabilizes each subunit (PubMed:29930106, PubMed:30397023).
CC {ECO:0000269|PubMed:23027666, ECO:0000269|PubMed:29438089,
CC ECO:0000269|PubMed:29930106, ECO:0000269|PubMed:30397023}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma
CC {ECO:0000269|PubMed:23027666}. Plastid, chloroplast thylakoid membrane
CC {ECO:0000269|PubMed:23027666}; Peripheral membrane protein
CC {ECO:0000269|PubMed:23027666}; Stromal side
CC {ECO:0000269|PubMed:23027666}. Note=Predominantly present at thylakoid
CC membranes. {ECO:0000269|PubMed:23027666}.
CC -!- DISRUPTION PHENOTYPE: Impaired photoautotrophy (PubMed:23027666).
CC Seedling lethal (PubMed:23027666). High chlorophyll fluorescence
CC phenotype and severely affected photosystem II (PSII) subunits
CC accumulation associated with a drastically decreased synthesis of the
CC reaction center protein D1 due to a reduced translation initiation
CC (ribosomal loading) of the corresponding psbA mRNA (PubMed:23027666).
CC Plants lacking both HCF173 and HCF244 display stronger PSII defects
CC (PubMed:23027666). {ECO:0000269|PubMed:23027666}.
CC -!- SIMILARITY: Belongs to the NmrA-type oxidoreductase family.
CC {ECO:0000305}.
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DR EMBL; AL022604; CAA18744.1; -; Genomic_DNA.
DR EMBL; AL161587; CAB80242.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE86485.1; -; Genomic_DNA.
DR EMBL; AF462834; AAL58922.1; -; mRNA.
DR EMBL; AY133542; AAM91372.1; -; mRNA.
DR PIR; T06132; T06132.
DR RefSeq; NP_195251.1; NM_119691.4.
DR AlphaFoldDB; O65502; -.
DR SMR; O65502; -.
DR IntAct; O65502; 5.
DR STRING; 3702.AT4G35250.1; -.
DR PaxDb; O65502; -.
DR PRIDE; O65502; -.
DR ProteomicsDB; 175118; -.
DR EnsemblPlants; AT4G35250.1; AT4G35250.1; AT4G35250.
DR GeneID; 829678; -.
DR Gramene; AT4G35250.1; AT4G35250.1; AT4G35250.
DR KEGG; ath:AT4G35250; -.
DR Araport; AT4G35250; -.
DR TAIR; locus:2122083; AT4G35250.
DR eggNOG; KOG1203; Eukaryota.
DR HOGENOM; CLU_007383_10_3_1; -.
DR InParanoid; O65502; -.
DR OMA; AYMNTQD; -.
DR OrthoDB; 707551at2759; -.
DR PhylomeDB; O65502; -.
DR PRO; PR:O65502; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; O65502; baseline and differential.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009570; C:chloroplast stroma; IDA:UniProtKB.
DR GO; GO:0009534; C:chloroplast thylakoid; IDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009523; C:photosystem II; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0042651; C:thylakoid membrane; IDA:UniProtKB.
DR GO; GO:0003743; F:translation initiation factor activity; IMP:TAIR.
DR GO; GO:0010207; P:photosystem II assembly; IMP:TAIR.
DR InterPro; IPR044256; HCF244-like.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR008030; NmrA-like.
DR PANTHER; PTHR47128; PTHR47128; 1.
DR Pfam; PF05368; NmrA; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Membrane; Photosynthesis; Photosystem II; Plastid;
KW Reference proteome; Thylakoid; Transit peptide.
FT TRANSIT 1..64
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 65..395
FT /note="Protein HIGH CHLOROPHYLL FLUORESCENCE PHENOTYPE 244,
FT chloroplastic"
FT /id="PRO_0000447638"
SQ SEQUENCE 395 AA; 43723 MW; 97A0BCCE420B55FB CRC64;
MASLRLPAQL VTRGNLIHHN SSSSSSGRLS WRRSLTPENT IPLFPSSSSS SLNRERSIVV
PVTCSAAAVN LAPGTPVRPT SILVVGATGT LGRQIVRRAL DEGYDVRCLV RPRPAPADFL
RDWGATVVNA DLSKPETIPA TLVGIHTVID CATGRPEEPI KTVDWEGKVA LIQCAKAMGI
QKYVFYSIHN CDKHPEVPLM EIKYCTEKFL QESGLNHITI RLCGFMQGLI GQYAVPILEE
KSVWGTDAPT RVAYMDTQDI ARLTLIALRN EKINGKLLTF AGPRAWTTQE VITLCERLAG
QDANVTTVPV SVLRVTRQLT RFFQWTNDVA DRLAFSEVLS SDTVFSAPMT ETNSLLGVDQ
KDMVTLEKYL QDYFSNILKK LKDLKAQSKQ SDIYF
