ID   HCAB_ECO5E              Reviewed;         270 AA.
AC   B5Z114;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   25-NOV-2008, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=3-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE            EC=1.3.1.87 {ECO:0000255|HAMAP-Rule:MF_01647};
DE   AltName: Full=2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE   AltName: Full=3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE   AltName: Full=CI-dihydrodiol dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE   AltName: Full=Cis-3-(2-carboxyethenyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE   AltName: Full=Cis-3-(2-carboxyethyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE   AltName: Full=PP-dihydrodiol dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
GN   Name=hcaB {ECO:0000255|HAMAP-Rule:MF_01647};
GN   OrderedLocusNames=ECH74115_3773;
OS   Escherichia coli O157:H7 (strain EC4115 / EHEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=444450;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=EC4115 / EHEC;
RX   PubMed=22135463; DOI=10.1073/pnas.1107176108;
RA   Eppinger M., Mammel M.K., Leclerc J.E., Ravel J., Cebula T.A.;
RT   "Genomic anatomy of Escherichia coli O157:H7 outbreaks.";
RL   Proc. Natl. Acad. Sci. U.S.A. 108:20142-20147(2011).
CC   -!- FUNCTION: Converts 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and
CC       cinnamic acid-dihydrodiol (CI-dihydrodiol) into 3-(2,3-
CC       dihydroxylphenyl)propanoic acid (DHPP) and 2,3-dihydroxicinnamic acid
CC       (DHCI), respectively. {ECO:0000255|HAMAP-Rule:MF_01647}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate +
CC         NAD(+) = 3-(2,3-dihydroxyphenyl)propanoate + H(+) + NADH;
CC         Xref=Rhea:RHEA:25062, ChEBI:CHEBI:15378, ChEBI:CHEBI:46951,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:60087; EC=1.3.1.87;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01647};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E)-3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)prop-2-enoate
CC         + NAD(+) = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + H(+) + NADH;
CC         Xref=Rhea:RHEA:25066, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:58642, ChEBI:CHEBI:61451; EC=1.3.1.87;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01647};
CC   -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropanoate degradation.
CC       {ECO:0000255|HAMAP-Rule:MF_01647}.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000255|HAMAP-Rule:MF_01647}.
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DR   EMBL; CP001164; ACI36557.1; -; Genomic_DNA.
DR   RefSeq; WP_001281372.1; NC_011353.1.
DR   AlphaFoldDB; B5Z114; -.
DR   SMR; B5Z114; -.
DR   KEGG; ecf:ECH74115_3773; -.
DR   HOGENOM; CLU_010194_1_0_6; -.
DR   OMA; VFHINVK; -.
DR   UniPathway; UPA00714; -.
DR   GO; GO:0018498; F:2,3-dihydroxy-2,3-dihydro-phenylpropionate dehydrogenase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019380; P:3-phenylpropionate catabolic process; IEA:UniProtKB-UniPathway.
DR   HAMAP; MF_01647; HcaB; 1.
DR   InterPro; IPR023643; Dihydrodiol_DH_HcaB.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; NAD; Oxidoreductase.
FT   CHAIN           1..270
FT                   /note="3-phenylpropionate-dihydrodiol/cinnamic acid-
FT                   dihydrodiol dehydrogenase"
FT                   /id="PRO_1000186964"
FT   ACT_SITE        156
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01647"
FT   BINDING         10..34
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01647"
FT   BINDING         143
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01647"
SQ   SEQUENCE   270 AA;  28440 MW;  6BFE56F10442C2A3 CRC64;
     MSDLHNESIF ITGGGSGLGL ALVERFIEEG AQVATLELSA AKVASLRQRF GEHILAVEGN
     VTCYADYQRA VDQILTRSGK LDCFIGNAGI WDHNASLVNT PAETLETGFH ELFNVNVLGY
     LLGAKACAPA LIAGEGSMIF TLSNAAWYPG GGGPLYTASK HAATGLIRQL AYELAPKVRV
     NGVGPCGMAS DLRGPQALGQ SETSIMQSLT PEKIAAILPL QFFPQPADFT GPYVMLASRR
     NNRALSGVMI NADAGLAIRG IRHVAAGLDL