ID   HCAB_ECO8A              Reviewed;         270 AA.
AC   B7M7P4;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=3-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE            EC=1.3.1.87 {ECO:0000255|HAMAP-Rule:MF_01647};
DE   AltName: Full=2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE   AltName: Full=3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE   AltName: Full=CI-dihydrodiol dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE   AltName: Full=Cis-3-(2-carboxyethenyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE   AltName: Full=Cis-3-(2-carboxyethyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE   AltName: Full=PP-dihydrodiol dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
GN   Name=hcaB {ECO:0000255|HAMAP-Rule:MF_01647}; OrderedLocusNames=ECIAI1_2593;
OS   Escherichia coli O8 (strain IAI1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=585034;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IAI1;
RX   PubMed=19165319; DOI=10.1371/journal.pgen.1000344;
RA   Touchon M., Hoede C., Tenaillon O., Barbe V., Baeriswyl S., Bidet P.,
RA   Bingen E., Bonacorsi S., Bouchier C., Bouvet O., Calteau A., Chiapello H.,
RA   Clermont O., Cruveiller S., Danchin A., Diard M., Dossat C., Karoui M.E.,
RA   Frapy E., Garry L., Ghigo J.M., Gilles A.M., Johnson J., Le Bouguenec C.,
RA   Lescat M., Mangenot S., Martinez-Jehanne V., Matic I., Nassif X., Oztas S.,
RA   Petit M.A., Pichon C., Rouy Z., Ruf C.S., Schneider D., Tourret J.,
RA   Vacherie B., Vallenet D., Medigue C., Rocha E.P.C., Denamur E.;
RT   "Organised genome dynamics in the Escherichia coli species results in
RT   highly diverse adaptive paths.";
RL   PLoS Genet. 5:E1000344-E1000344(2009).
CC   -!- FUNCTION: Converts 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and
CC       cinnamic acid-dihydrodiol (CI-dihydrodiol) into 3-(2,3-
CC       dihydroxylphenyl)propanoic acid (DHPP) and 2,3-dihydroxicinnamic acid
CC       (DHCI), respectively. {ECO:0000255|HAMAP-Rule:MF_01647}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate +
CC         NAD(+) = 3-(2,3-dihydroxyphenyl)propanoate + H(+) + NADH;
CC         Xref=Rhea:RHEA:25062, ChEBI:CHEBI:15378, ChEBI:CHEBI:46951,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:60087; EC=1.3.1.87;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01647};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E)-3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)prop-2-enoate
CC         + NAD(+) = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + H(+) + NADH;
CC         Xref=Rhea:RHEA:25066, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:58642, ChEBI:CHEBI:61451; EC=1.3.1.87;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01647};
CC   -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropanoate degradation.
CC       {ECO:0000255|HAMAP-Rule:MF_01647}.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000255|HAMAP-Rule:MF_01647}.
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DR   EMBL; CU928160; CAQ99432.1; -; Genomic_DNA.
DR   RefSeq; WP_001281385.1; NC_011741.1.
DR   AlphaFoldDB; B7M7P4; -.
DR   SMR; B7M7P4; -.
DR   KEGG; ecr:ECIAI1_2593; -.
DR   HOGENOM; CLU_010194_1_0_6; -.
DR   OMA; VFHINVK; -.
DR   UniPathway; UPA00714; -.
DR   GO; GO:0018498; F:2,3-dihydroxy-2,3-dihydro-phenylpropionate dehydrogenase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019380; P:3-phenylpropionate catabolic process; IEA:UniProtKB-UniPathway.
DR   HAMAP; MF_01647; HcaB; 1.
DR   InterPro; IPR023643; Dihydrodiol_DH_HcaB.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; NAD; Oxidoreductase.
FT   CHAIN           1..270
FT                   /note="3-phenylpropionate-dihydrodiol/cinnamic acid-
FT                   dihydrodiol dehydrogenase"
FT                   /id="PRO_1000186966"
FT   ACT_SITE        156
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01647"
FT   BINDING         10..34
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01647"
FT   BINDING         143
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01647"
SQ   SEQUENCE   270 AA;  28486 MW;  C1885C43DE42D3F1 CRC64;
     MSDLHNESIF ITGGGSGLGL ALVERFIEEG AQVATLELSA AKVASLRQRF GEHILAVEGN
     VTCYADYQRA VDQILTRSGK LDCFIGNAGI WDHNASLVNT PAETLETGFH ELFNVNVLGY
     LLGAKACAPA LISSEGSMIF TLSNAAWYPG GGGPLYTASK HAATGLIRQL AYELAPKVRV
     NGVGPCGMAS DLRGPQALGQ SETSIMQSLT PEKIAAILPL QFFPQPADFT GPYVMLASRR
     NNRALSGVMI NADAGLAIRG IRHVAAGLDL