HCAB_ECOBW
ID HCAB_ECOBW Reviewed; 270 AA.
AC C4ZXB6;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 28-JUL-2009, sequence version 1.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=3-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE EC=1.3.1.87 {ECO:0000255|HAMAP-Rule:MF_01647};
DE AltName: Full=2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE AltName: Full=3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE AltName: Full=CI-dihydrodiol dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE AltName: Full=Cis-3-(2-carboxyethenyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE AltName: Full=Cis-3-(2-carboxyethyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE AltName: Full=PP-dihydrodiol dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
GN Name=hcaB {ECO:0000255|HAMAP-Rule:MF_01647}; OrderedLocusNames=BWG_2305;
OS Escherichia coli (strain K12 / MC4100 / BW2952).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=595496;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MC4100 / BW2952;
RX PubMed=19376874; DOI=10.1128/jb.00118-09;
RA Ferenci T., Zhou Z., Betteridge T., Ren Y., Liu Y., Feng L., Reeves P.R.,
RA Wang L.;
RT "Genomic sequencing reveals regulatory mutations and recombinational events
RT in the widely used MC4100 lineage of Escherichia coli K-12.";
RL J. Bacteriol. 191:4025-4029(2009).
CC -!- FUNCTION: Converts 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and
CC cinnamic acid-dihydrodiol (CI-dihydrodiol) into 3-(2,3-
CC dihydroxylphenyl)propanoic acid (DHPP) and 2,3-dihydroxicinnamic acid
CC (DHCI), respectively. {ECO:0000255|HAMAP-Rule:MF_01647}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate +
CC NAD(+) = 3-(2,3-dihydroxyphenyl)propanoate + H(+) + NADH;
CC Xref=Rhea:RHEA:25062, ChEBI:CHEBI:15378, ChEBI:CHEBI:46951,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:60087; EC=1.3.1.87;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01647};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)prop-2-enoate
CC + NAD(+) = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + H(+) + NADH;
CC Xref=Rhea:RHEA:25066, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945, ChEBI:CHEBI:58642, ChEBI:CHEBI:61451; EC=1.3.1.87;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01647};
CC -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropanoate degradation.
CC {ECO:0000255|HAMAP-Rule:MF_01647}.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000255|HAMAP-Rule:MF_01647}.
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DR EMBL; CP001396; ACR61743.1; -; Genomic_DNA.
DR RefSeq; WP_001281379.1; NC_012759.1.
DR AlphaFoldDB; C4ZXB6; -.
DR SMR; C4ZXB6; -.
DR KEGG; ebw:BWG_2305; -.
DR HOGENOM; CLU_010194_1_0_6; -.
DR OMA; VFHINVK; -.
DR UniPathway; UPA00714; -.
DR GO; GO:0018498; F:2,3-dihydroxy-2,3-dihydro-phenylpropionate dehydrogenase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019380; P:3-phenylpropionate catabolic process; IEA:UniProtKB-UniPathway.
DR HAMAP; MF_01647; HcaB; 1.
DR InterPro; IPR023643; Dihydrodiol_DH_HcaB.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR InterPro; IPR002347; SDR_fam.
DR Pfam; PF00106; adh_short; 1.
DR PRINTS; PR00081; GDHRDH.
DR PRINTS; PR00080; SDRFAMILY.
DR SUPFAM; SSF51735; SSF51735; 1.
DR PROSITE; PS00061; ADH_SHORT; 1.
PE 3: Inferred from homology;
KW Aromatic hydrocarbons catabolism; NAD; Oxidoreductase.
FT CHAIN 1..270
FT /note="3-phenylpropionate-dihydrodiol/cinnamic acid-
FT dihydrodiol dehydrogenase"
FT /id="PRO_1000215849"
FT ACT_SITE 156
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01647"
FT BINDING 10..34
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01647"
FT BINDING 143
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01647"
SQ SEQUENCE 270 AA; 28500 MW; 607D316E7442D3E7 CRC64;
MSDLHNESIF ITGGGSGLGL ALVERFIEEG AQVATLELSA AKVASLRQRF GEHILAVEGN
VTCYADYQRA VDQILTRSGK LDCFIGNAGI WDHNASLVNT PAETLETGFH ELFNVNVLGY
LLGAKACAPA LIASEGSMIF TLSNAAWYPG GGGPLYTASK HAATGLIRQL AYELAPKVRV
NGVGPCGMAS DLRGPQALGQ SETSIMQSLT PEKIAAILPL QFFPQPADFT GPYVMLTSRR
NNRALSGVMI NADAGLAIRG IRHVAAGLDL
