HCAB_ECOLC
ID HCAB_ECOLC Reviewed; 270 AA.
AC B1IVT6;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 29-APR-2008, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=3-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE EC=1.3.1.87 {ECO:0000255|HAMAP-Rule:MF_01647};
DE AltName: Full=2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE AltName: Full=3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE AltName: Full=CI-dihydrodiol dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE AltName: Full=Cis-3-(2-carboxyethenyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE AltName: Full=Cis-3-(2-carboxyethyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE AltName: Full=PP-dihydrodiol dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
GN Name=hcaB {ECO:0000255|HAMAP-Rule:MF_01647}; OrderedLocusNames=EcolC_1136;
OS Escherichia coli (strain ATCC 8739 / DSM 1576 / NBRC 3972 / NCIMB 8545 /
OS WDCM 00012 / Crooks).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=481805;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 8739 / DSM 1576 / NBRC 3972 / NCIMB 8545 / WDCM 00012 / Crooks;
RA Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C.,
RA Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA Mikhailova N., Ingram L., Richardson P.;
RT "Complete sequence of Escherichia coli C str. ATCC 8739.";
RL Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Converts 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and
CC cinnamic acid-dihydrodiol (CI-dihydrodiol) into 3-(2,3-
CC dihydroxylphenyl)propanoic acid (DHPP) and 2,3-dihydroxicinnamic acid
CC (DHCI), respectively. {ECO:0000255|HAMAP-Rule:MF_01647}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate +
CC NAD(+) = 3-(2,3-dihydroxyphenyl)propanoate + H(+) + NADH;
CC Xref=Rhea:RHEA:25062, ChEBI:CHEBI:15378, ChEBI:CHEBI:46951,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:60087; EC=1.3.1.87;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01647};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)prop-2-enoate
CC + NAD(+) = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + H(+) + NADH;
CC Xref=Rhea:RHEA:25066, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945, ChEBI:CHEBI:58642, ChEBI:CHEBI:61451; EC=1.3.1.87;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01647};
CC -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropanoate degradation.
CC {ECO:0000255|HAMAP-Rule:MF_01647}.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000255|HAMAP-Rule:MF_01647}.
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DR EMBL; CP000946; ACA76803.1; -; Genomic_DNA.
DR RefSeq; WP_001281377.1; NZ_CP022959.1.
DR AlphaFoldDB; B1IVT6; -.
DR SMR; B1IVT6; -.
DR GeneID; 66673571; -.
DR KEGG; ecl:EcolC_1136; -.
DR HOGENOM; CLU_010194_1_0_6; -.
DR OMA; VFHINVK; -.
DR UniPathway; UPA00714; -.
DR GO; GO:0018498; F:2,3-dihydroxy-2,3-dihydro-phenylpropionate dehydrogenase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019380; P:3-phenylpropionate catabolic process; IEA:UniProtKB-UniPathway.
DR HAMAP; MF_01647; HcaB; 1.
DR InterPro; IPR023643; Dihydrodiol_DH_HcaB.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR InterPro; IPR002347; SDR_fam.
DR Pfam; PF00106; adh_short; 1.
DR PRINTS; PR00081; GDHRDH.
DR PRINTS; PR00080; SDRFAMILY.
DR SUPFAM; SSF51735; SSF51735; 1.
DR PROSITE; PS00061; ADH_SHORT; 1.
PE 3: Inferred from homology;
KW Aromatic hydrocarbons catabolism; NAD; Oxidoreductase.
FT CHAIN 1..270
FT /note="3-phenylpropionate-dihydrodiol/cinnamic acid-
FT dihydrodiol dehydrogenase"
FT /id="PRO_1000186968"
FT ACT_SITE 156
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01647"
FT BINDING 10..34
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01647"
FT BINDING 143
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01647"
SQ SEQUENCE 270 AA; 28470 MW; 606087B50442D3E7 CRC64;
MSDLHNESIF ITGGGSGLGL ALVERFIEEG AQVATLELSA AKVASLRQRF GEHILAVEGN
VTCYADYQRA VDQILTRSGK LDCFIGNAGI WDHNASLVNT PAETLETGFH ELFNVNVLGY
LLGAKACAPA LIASEGSMIF TLSNAAWYPG GGGPLYTASK HAATGLIRQL AYELAPKVRV
NGVGPCGMAS DLRGPQALGQ SETSIMQSLT PEKIAAILPL QFFPQPADFT GPYVMLASRR
NNRALSGVMI NADAGLAIRG IRHVAAGLDL
