ID   HCAB_ECOLX              Reviewed;         270 AA.
AC   P0CI32; P76995; P77646;
DT   30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   30-NOV-2010, sequence version 1.
DT   03-AUG-2022, entry version 46.
DE   RecName: Full=3-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenase;
DE            EC=1.3.1.87;
DE   AltName: Full=2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase;
DE   AltName: Full=3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate dehydrogenase;
DE   AltName: Full=CI-dihydrodiol dehydrogenase;
DE   AltName: Full=Cis-3-(2-carboxyethenyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase;
DE   AltName: Full=Cis-3-(2-carboxyethyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase;
DE   AltName: Full=PP-dihydrodiol dehydrogenase;
GN   Name=hcaB;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN CATABOLISM OF
RP   PHENYLPROPIONIC AND CINNAMIC ACIDS, AND CATALYTIC ACTIVITY.
RC   STRAIN=K12 / MC1061 / ATCC 53338 / DSM 7140;
RX   PubMed=9603882; DOI=10.1128/jb.180.11.2915-2923.1998;
RA   Diaz E., Ferrandez A., Garcia J.L.;
RT   "Characterization of the hca cluster encoding the dioxygenolytic pathway
RT   for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-
RT   12.";
RL   J. Bacteriol. 180:2915-2923(1998).
CC   -!- FUNCTION: Converts 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and
CC       cinnamic acid-dihydrodiol (CI-dihydrodiol) into 3-(2,3-
CC       dihydroxylphenyl)propanoic acid (DHPP) and 2,3-dihydroxicinnamic acid
CC       (DHCI), respectively. {ECO:0000269|PubMed:9603882}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate +
CC         NAD(+) = 3-(2,3-dihydroxyphenyl)propanoate + H(+) + NADH;
CC         Xref=Rhea:RHEA:25062, ChEBI:CHEBI:15378, ChEBI:CHEBI:46951,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:60087; EC=1.3.1.87;
CC         Evidence={ECO:0000269|PubMed:9603882};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E)-3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)prop-2-enoate
CC         + NAD(+) = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + H(+) + NADH;
CC         Xref=Rhea:RHEA:25066, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:58642, ChEBI:CHEBI:61451; EC=1.3.1.87;
CC         Evidence={ECO:0000269|PubMed:9603882};
CC   -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropanoate degradation.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; Y11070; CAA71951.1; -; Genomic_DNA.
DR   AlphaFoldDB; P0CI32; -.
DR   SMR; P0CI32; -.
DR   STRING; 585034.ECIAI1_2593; -.
DR   eggNOG; COG1028; Bacteria.
DR   UniPathway; UPA00714; -.
DR   GO; GO:0018498; F:2,3-dihydroxy-2,3-dihydro-phenylpropionate dehydrogenase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019380; P:3-phenylpropionate catabolic process; IEA:UniProtKB-UniPathway.
DR   HAMAP; MF_01647; HcaB; 1.
DR   InterPro; IPR023643; Dihydrodiol_DH_HcaB.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   1: Evidence at protein level;
KW   Aromatic hydrocarbons catabolism; NAD; Oxidoreductase.
FT   CHAIN           1..270
FT                   /note="3-phenylpropionate-dihydrodiol/cinnamic acid-
FT                   dihydrodiol dehydrogenase"
FT                   /id="PRO_0000402179"
FT   ACT_SITE        156
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         10..34
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         143
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   270 AA;  28499 MW;  607FBBC4DEECD3E7 CRC64;
     MSDLHNESIF ITGGGSGLGL ALVERFIEKG AQVATLELSA AKVASLRQRF GEHILAVEGN
     VTCYADYQRA VDQILTRSGK LDCFIGNAGI WDHNASLVNT PAETLETGFH ELFNVNVLGY
     LLGAKACAPA LIASEGSMIF TLSNAAWYPG GGGPLYTASK HAATGLIRQL AYELAPKVRV
     NGVGPCGMAS DLRGPQALGQ SETSIMQSLT PEKIAAILPL QFFPQPADFT GPYVMLTSRR
     NNRALSGVMI NADAGLAIRG IRHVAAGLDL