HCAB_SHIF8
ID HCAB_SHIF8 Reviewed; 270 AA.
AC Q0T1X8;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=3-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE EC=1.3.1.87 {ECO:0000255|HAMAP-Rule:MF_01647};
DE AltName: Full=2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE AltName: Full=3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE AltName: Full=CI-dihydrodiol dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE AltName: Full=Cis-3-(2-carboxyethenyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE AltName: Full=Cis-3-(2-carboxyethyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
DE AltName: Full=PP-dihydrodiol dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01647};
GN Name=hcaB {ECO:0000255|HAMAP-Rule:MF_01647}; OrderedLocusNames=SFV_2589;
OS Shigella flexneri serotype 5b (strain 8401).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=373384;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=8401;
RX PubMed=16822325; DOI=10.1186/1471-2164-7-173;
RA Nie H., Yang F., Zhang X., Yang J., Chen L., Wang J., Xiong Z., Peng J.,
RA Sun L., Dong J., Xue Y., Xu X., Chen S., Yao Z., Shen Y., Jin Q.;
RT "Complete genome sequence of Shigella flexneri 5b and comparison with
RT Shigella flexneri 2a.";
RL BMC Genomics 7:173-173(2006).
CC -!- FUNCTION: Converts 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and
CC cinnamic acid-dihydrodiol (CI-dihydrodiol) into 3-(2,3-
CC dihydroxylphenyl)propanoic acid (DHPP) and 2,3-dihydroxicinnamic acid
CC (DHCI), respectively. {ECO:0000255|HAMAP-Rule:MF_01647}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate +
CC NAD(+) = 3-(2,3-dihydroxyphenyl)propanoate + H(+) + NADH;
CC Xref=Rhea:RHEA:25062, ChEBI:CHEBI:15378, ChEBI:CHEBI:46951,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:60087; EC=1.3.1.87;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01647};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)prop-2-enoate
CC + NAD(+) = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + H(+) + NADH;
CC Xref=Rhea:RHEA:25066, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945, ChEBI:CHEBI:58642, ChEBI:CHEBI:61451; EC=1.3.1.87;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01647};
CC -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropanoate degradation.
CC {ECO:0000255|HAMAP-Rule:MF_01647}.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000255|HAMAP-Rule:MF_01647}.
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DR EMBL; CP000266; ABF04687.1; -; Genomic_DNA.
DR RefSeq; WP_001281382.1; NC_008258.1.
DR AlphaFoldDB; Q0T1X8; -.
DR SMR; Q0T1X8; -.
DR EnsemblBacteria; ABF04687; ABF04687; SFV_2589.
DR GeneID; 58390240; -.
DR KEGG; sfv:SFV_2589; -.
DR HOGENOM; CLU_010194_1_0_6; -.
DR OMA; VFHINVK; -.
DR BioCyc; SFLE373384:SFV_RS14395-MON; -.
DR UniPathway; UPA00714; -.
DR Proteomes; UP000000659; Chromosome.
DR GO; GO:0018498; F:2,3-dihydroxy-2,3-dihydro-phenylpropionate dehydrogenase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019380; P:3-phenylpropionate catabolic process; IEA:UniProtKB-UniPathway.
DR HAMAP; MF_01647; HcaB; 1.
DR InterPro; IPR023643; Dihydrodiol_DH_HcaB.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR InterPro; IPR002347; SDR_fam.
DR Pfam; PF00106; adh_short; 1.
DR PRINTS; PR00081; GDHRDH.
DR SUPFAM; SSF51735; SSF51735; 1.
DR PROSITE; PS00061; ADH_SHORT; 1.
PE 3: Inferred from homology;
KW Aromatic hydrocarbons catabolism; NAD; Oxidoreductase.
FT CHAIN 1..270
FT /note="3-phenylpropionate-dihydrodiol/cinnamic acid-
FT dihydrodiol dehydrogenase"
FT /id="PRO_0000333766"
FT ACT_SITE 156
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01647"
FT BINDING 10..34
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01647"
FT BINDING 143
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01647"
SQ SEQUENCE 270 AA; 28500 MW; 8FCA37B50442C94D CRC64;
MSDLHNESIF ITGGGSGLGL ALVERFIEEG AQVATLELSA AKVASLRQRF GEHILAVEGN
VTCYADYQRA VDQILTRSGK LDCFIGNAGI WDHNASLVNT PAETLETGFH ELFNVNVLGY
LLGAKACAPA LIASEGSMIF TLSNAAWYPG GGGPLYTTSK HAATGLIRQL AYELAPKVRV
NGVGPCGMAS DLRGPQALGQ SETSIMQSLT PEKIAAILPL QFFPQPADFT GPYVMLASRR
NNRALSGVMI NADAGLAIRG IRHVAAGLDL
