HCAF_ECOLI
ID HCAF_ECOLI Reviewed; 172 AA.
AC Q47140; P77508;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=3-phenylpropionate/cinnamic acid dioxygenase subunit beta;
DE EC=1.14.12.19;
GN Name=hcaF; Synonyms=digB, hcaA2, hcaB, phdC2, yfhV;
GN OrderedLocusNames=b2539, JW2523;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-49.
RC STRAIN=K12;
RA Turlin E., Gasser F., Biville F.;
RL Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 42-172, AND FUNCTION IN CATABOLISM OF
RP PHENYLPROPIONIC AND CINNAMIC ACIDS.
RC STRAIN=K12 / MC1061 / ATCC 53338 / DSM 7140;
RX PubMed=9603882; DOI=10.1128/jb.180.11.2915-2923.1998;
RA Diaz E., Ferrandez A., Garcia J.L.;
RT "Characterization of the hca cluster encoding the dioxygenolytic pathway
RT for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-
RT 12.";
RL J. Bacteriol. 180:2915-2923(1998).
CC -!- FUNCTION: Part of the multicomponent 3-phenylpropionate dioxygenase.
CC Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-
CC phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-
CC dihydrodiol (CI-dihydrodiol), respectively.
CC {ECO:0000269|PubMed:9603882}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-phenylpropanoate + H(+) + NADH + O2 = 3-(cis-5,6-
CC dihydroxycyclohexa-1,3-dien-1-yl)propanoate + NAD(+);
CC Xref=Rhea:RHEA:20357, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:51057, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC ChEBI:CHEBI:60087; EC=1.14.12.19;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(E)-cinnamate + H(+) + NADH + O2 = (2E)-3-(cis-5,6-
CC dihydroxycyclohexa-1,3-dien-1-yl)prop-2-enoate + NAD(+);
CC Xref=Rhea:RHEA:25058, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:15669, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC ChEBI:CHEBI:61451; EC=1.14.12.19;
CC -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropanoate degradation.
CC -!- SUBUNIT: This dioxygenase system consists of four proteins: the two
CC subunits of the hydroxylase component (HcaE and HcaF), a ferredoxin
CC (HcaC) and a ferredoxin reductase (HcaD).
CC -!- SIMILARITY: Belongs to the bacterial ring-hydroxylating dioxygenase
CC beta subunit family. {ECO:0000305}.
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DR EMBL; U00096; AAC75592.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA16442.1; -; Genomic_DNA.
DR EMBL; Z37966; CAA86019.1; -; Genomic_DNA.
DR EMBL; Y11070; CAA71949.1; -; Genomic_DNA.
DR PIR; B65031; B65031.
DR RefSeq; NP_417034.1; NC_000913.3.
DR RefSeq; WP_001276072.1; NZ_STEB01000011.1.
DR AlphaFoldDB; Q47140; -.
DR SMR; Q47140; -.
DR BioGRID; 4261576; 29.
DR ComplexPortal; CPX-5161; 3-phenylpropionate/cinnamic acid dioxygenase.
DR STRING; 511145.b2539; -.
DR PaxDb; Q47140; -.
DR PRIDE; Q47140; -.
DR EnsemblBacteria; AAC75592; AAC75592; b2539.
DR EnsemblBacteria; BAA16442; BAA16442; BAA16442.
DR GeneID; 66673573; -.
DR GeneID; 946997; -.
DR KEGG; ecj:JW2523; -.
DR KEGG; eco:b2539; -.
DR PATRIC; fig|1411691.4.peg.4195; -.
DR EchoBASE; EB3230; -.
DR eggNOG; COG5517; Bacteria.
DR HOGENOM; CLU_102527_1_1_6; -.
DR InParanoid; Q47140; -.
DR OMA; LQAHQFL; -.
DR PhylomeDB; Q47140; -.
DR BioCyc; EcoCyc:HCAA2-MON; -.
DR BioCyc; MetaCyc:HCAA2-MON; -.
DR UniPathway; UPA00714; -.
DR PRO; PR:Q47140; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0009334; C:3-phenylpropionate dioxygenase complex; IC:ComplexPortal.
DR GO; GO:0008695; F:3-phenylpropionate dioxygenase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019380; P:3-phenylpropionate catabolic process; IMP:EcoCyc.
DR CDD; cd00667; ring_hydroxylating_dioxygenases_beta; 1.
DR HAMAP; MF_01649; HcaF; 1.
DR InterPro; IPR023712; HcaF.
DR InterPro; IPR032710; NTF2-like_dom_sf.
DR InterPro; IPR000391; Rng_hydr_dOase-bsu.
DR PANTHER; PTHR41534; PTHR41534; 1.
DR Pfam; PF00866; Ring_hydroxyl_B; 1.
DR SUPFAM; SSF54427; SSF54427; 1.
PE 1: Evidence at protein level;
KW Aromatic hydrocarbons catabolism; Dioxygenase; NAD; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..172
FT /note="3-phenylpropionate/cinnamic acid dioxygenase subunit
FT beta"
FT /id="PRO_0000085077"
FT CONFLICT 11
FT /note="H -> Y (in Ref. 4; CAA86019)"
FT /evidence="ECO:0000305"
FT CONFLICT 49
FT /note="N -> D (in Ref. 4; CAA86019)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 172 AA; 20579 MW; 83833B22A75BE546 CRC64;
MSAQVSLELH HRISQFLFHE ASLLDDWKFR DWLAQLDEEI RYTMRTTVNA QTRDRRKGVQ
PPTTWIFNDT KDQLERRIAR LETGMAWAEE PPSRTRHLIS NCQISETDIP NVFAVRVNYL
LYRAQKERDE TFYVGTRFDK VRRLEDDNWR LLERDIVLDQ AVITSHNLSV LF
