ANDR_PANTR
ID ANDR_PANTR Reviewed; 911 AA.
AC O97775;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Androgen receptor;
DE AltName: Full=Dihydrotestosterone receptor;
DE AltName: Full=Nuclear receptor subfamily 3 group C member 4;
GN Name=AR; Synonyms=NR3C4;
OS Pan troglodytes (Chimpanzee).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Pan.
OX NCBI_TaxID=9598;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9732460; DOI=10.1007/pl00006391;
RA Choong C.S., Kemppainen J.A., Wilson E.M.;
RT "Evolution of the primate androgen receptor: a structural basis for
RT disease.";
RL J. Mol. Evol. 47:334-342(1998).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS) OF 654-911 IN COMPLEXES WITH
RP DIHYDROTESTOSTERONE AND COACTIVATOR-BASED PEPTIDES.
RX PubMed=15328534; DOI=10.1371/journal.pbio.0020274;
RA Hur E., Pfaff S.J., Payne E.S., Groen H., Buehrer B.M., Fletterick R.J.;
RT "Recognition and accommodation at the androgen receptor coactivator binding
RT interface.";
RL PLoS Biol. 2:E274-E274(2004).
CC -!- FUNCTION: Steroid hormone receptors are ligand-activated transcription
CC factors that regulate eukaryotic gene expression and affect cellular
CC proliferation and differentiation in target tissues. Transcription
CC factor activity is modulated by bound coactivator and corepressor
CC proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen
CC response elements/ARE on target genes, negatively regulating androgen
CC receptor signaling and androgen-induced cell proliferation.
CC Transcription activation is also down-regulated by NR0B2. Activated,
CC but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
CC {ECO:0000250|UniProtKB:P10275, ECO:0000250|UniProtKB:P15207}.
CC -!- SUBUNIT: Binds DNA as a homodimer. Part of a ternary complex containing
CC AR, EFCAB6/DJBP and PARK7. Interacts with HIPK3 and NR0B2 in the
CC presence of androgen. The ligand binding domain interacts with
CC KAT7/HBO1 in the presence of dihydrotestosterone. Interacts with
CC EFCAB6/DJBP, PQBP1, RANBP9, RBAK, SPDEF, SRA1, TGFB1I1 and RREB1.
CC Interacts with ZMIZ1/ZIMP10 and ZMIZ2/ZMIP7 which both enhance its
CC transactivation activity. Interacts with SLC30A9 and RAD54L2/ARIP4.
CC Interacts with MACROD1 (via macro domain) (By similarity). Interacts
CC via the ligand-binding domain with LXXLL and FXXLF motifs from NCOA1,
CC NCOA2, NCOA3, NCOA4 and MAGEA11. The AR N-terminal poly-Gln region
CC binds Ran resulting in enhancement of AR-mediated transactivation. Ran-
CC binding decreases as the poly-Gln length increases. Interacts with HIP1
CC (via coiled coil domain). Interacts (via ligand-binding domain) with
CC TRIM68. Interacts with TNK2. Interacts with USP26. Interacts with RNF6.
CC Interacts (regulated by RNF6 probably through polyubiquitination) with
CC RNF14; regulates AR transcriptional activity. Interacts with PRMT2 and
CC TRIM24. Interacts with RACK1. Interacts with RANBP10; this interaction
CC enhances dihydrotestosterone-induced AR transcriptional activity.
CC Interacts with PRPF6 in a hormone-independent way; this interaction
CC enhances dihydrotestosterone-induced AR transcriptional activity.
CC Interacts with STK4/MST1. Interacts with ZIPK/DAPK3. Interacts with
CC LPXN. Interacts with MAK. Part of a complex containing AR, MAK and
CC NCOA3. Interacts with CRY1. Interacts with CCAR1 and GATA2. Interacts
CC with ZNF318. Interacts with BUD31. Interacts with ARID4A. Interacts
CC with ARID4B. Interacts (via NR LBD domain) with ZBTB7A; the interaction
CC is direct and androgen-dependent (By similarity). Interacts with NCOR1
CC (By similarity). Interacts with NCOR2 (By similarity). Interacts with
CC CRY2 in a ligand-dependent manner (By similarity).
CC {ECO:0000250|UniProtKB:P10275, ECO:0000250|UniProtKB:P15207,
CC ECO:0000250|UniProtKB:P19091}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P10275}. Cytoplasm
CC {ECO:0000250|UniProtKB:P10275}. Note=Detected at the promoter of target
CC genes. Predominantly cytoplasmic in unligated form but translocates to
CC the nucleus upon ligand-binding. Can also translocate to the nucleus in
CC unligated form in the presence of RACK1.
CC {ECO:0000250|UniProtKB:P10275}.
CC -!- DOMAIN: Composed of three domains: a modulating N-terminal domain, a
CC DNA-binding domain and a C-terminal ligand-binding domain. In the
CC presence of bound steroid the ligand-binding domain interacts with the
CC N-terminal modulating domain, and thereby activates AR transcription
CC factor activity. Agonist binding is required for dimerization and
CC binding to target DNA. The transcription factor activity of the complex
CC formed by ligand-activated AR and DNA is modulated by interactions with
CC coactivator and corepressor proteins. Interaction with RANBP9 is
CC mediated by both the N-terminal domain and the DNA-binding domain.
CC Interaction with EFCAB6/DJBP is mediated by the DNA-binding domain (By
CC similarity). {ECO:0000250}.
CC -!- PTM: Phosphorylated in prostate cancer cells in response to several
CC growth factors including EGF. Phosphorylation is induced by c-Src
CC kinase (CSK). Tyr-526 is one of the major phosphorylation sites and an
CC increase in phosphorylation and Src kinase activity is associated with
CC prostate cancer progression (By similarity). Phosphorylation by TNK2
CC enhances the DNA-binding and transcriptional activity. Phosphorylation
CC at Ser-81 by CDK9 regulates AR promoter selectivity and cell growth (By
CC similarity). {ECO:0000250|UniProtKB:P10275}.
CC -!- PTM: Sumoylated on Lys-385 (major) and Lys-512 (By similarity).
CC Ubiquitinated. Deubiquitinated by USP26 (By similarity). 'Lys-6' and
CC 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional
CC activity and specificity (By similarity).
CC {ECO:0000250|UniProtKB:P10275}.
CC -!- PTM: Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required
CC for plasma membrane targeting and for rapid intracellular signaling via
CC ERK and AKT kinases and cAMP generation (By similarity).
CC {ECO:0000250|UniProtKB:P10275}.
CC -!- MISCELLANEOUS: In the absence of ligand, steroid hormone receptors are
CC thought to be weakly associated with nuclear components; hormone
CC binding greatly increases receptor affinity. The hormone-receptor
CC complex appears to recognize discrete DNA sequences upstream of
CC transcriptional start sites.
CC -!- MISCELLANEOUS: Transcriptional activity is enhanced by binding to
CC RANBP9.
CC -!- SIMILARITY: Belongs to the nuclear hormone receptor family. NR3
CC subfamily. {ECO:0000305}.
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DR EMBL; U94177; AAC73048.1; -; mRNA.
DR RefSeq; NP_001009012.1; NM_001009012.1.
DR PDB; 1T73; X-ray; 2.20 A; A=654-911.
DR PDB; 1T74; X-ray; 2.00 A; A=654-911.
DR PDB; 1T76; X-ray; 2.10 A; A=654-911.
DR PDB; 1T79; X-ray; 1.80 A; A=654-911.
DR PDB; 1T7F; X-ray; 1.60 A; A=654-911.
DR PDB; 1T7M; X-ray; 1.60 A; A=654-911.
DR PDB; 1T7R; X-ray; 1.40 A; A=654-911.
DR PDB; 1T7T; X-ray; 1.70 A; A=654-911.
DR PDBsum; 1T73; -.
DR PDBsum; 1T74; -.
DR PDBsum; 1T76; -.
DR PDBsum; 1T79; -.
DR PDBsum; 1T7F; -.
DR PDBsum; 1T7M; -.
DR PDBsum; 1T7R; -.
DR PDBsum; 1T7T; -.
DR AlphaFoldDB; O97775; -.
DR SMR; O97775; -.
DR DIP; DIP-46077N; -.
DR PaxDb; O97775; -.
DR GeneID; 747460; -.
DR KEGG; ptr:747460; -.
DR CTD; 367; -.
DR eggNOG; KOG3575; Eukaryota.
DR InParanoid; O97775; -.
DR OrthoDB; 615449at2759; -.
DR EvolutionaryTrace; O97775; -.
DR Proteomes; UP000002277; Unplaced.
DR GO; GO:0000785; C:chromatin; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProt.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005497; F:androgen binding; ISS:UniProtKB.
DR GO; GO:0008013; F:beta-catenin binding; ISS:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:UniProtKB.
DR GO; GO:0004879; F:nuclear receptor activity; ISS:UniProtKB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0005496; F:steroid binding; IEA:UniProtKB-KW.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; ISS:UniProtKB.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0030521; P:androgen receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:0030522; P:intracellular receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:0030518; P:intracellular steroid hormone receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0008584; P:male gonad development; IBA:GO_Central.
DR GO; GO:2001237; P:negative regulation of extrinsic apoptotic signaling pathway; ISS:UniProtKB.
DR GO; GO:0045720; P:negative regulation of integrin biosynthetic process; ISS:UniProtKB.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; ISS:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; ISS:UniProtKB.
DR GO; GO:0051092; P:positive regulation of NF-kappaB transcription factor activity; ISS:UniProtKB.
DR GO; GO:0042327; P:positive regulation of phosphorylation; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:1903076; P:regulation of protein localization to plasma membrane; ISS:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.565.10; -; 1.
DR Gene3D; 3.30.50.10; -; 1.
DR InterPro; IPR001103; Andrgn_rcpt.
DR InterPro; IPR035500; NHR-like_dom_sf.
DR InterPro; IPR000536; Nucl_hrmn_rcpt_lig-bd.
DR InterPro; IPR001628; Znf_hrmn_rcpt.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR Pfam; PF02166; Androgen_recep; 1.
DR Pfam; PF00104; Hormone_recep; 1.
DR Pfam; PF00105; zf-C4; 1.
DR PRINTS; PR00521; ANDROGENR.
DR PRINTS; PR00047; STROIDFINGER.
DR SMART; SM00430; HOLI; 1.
DR SMART; SM00399; ZnF_C4; 1.
DR SUPFAM; SSF48508; SSF48508; 1.
DR PROSITE; PS51843; NR_LBD; 1.
DR PROSITE; PS00031; NUCLEAR_REC_DBD_1; 1.
DR PROSITE; PS51030; NUCLEAR_REC_DBD_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; DNA-binding; Isopeptide bond; Lipid-binding;
KW Lipoprotein; Metal-binding; Nucleus; Palmitate; Phosphoprotein; Receptor;
KW Reference proteome; Steroid-binding; Transcription;
KW Transcription regulation; Ubl conjugation; Zinc; Zinc-finger.
FT CHAIN 1..911
FT /note="Androgen receptor"
FT /id="PRO_0000053708"
FT DOMAIN 660..891
FT /note="NR LBD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01189"
FT DNA_BIND 551..623
FT /note="Nuclear receptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 551..571
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 587..611
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT REGION 1..578
FT /note="Interaction with ZNF318"
FT /evidence="ECO:0000250|UniProtKB:P19091"
FT REGION 1..549
FT /note="Modulating"
FT /evidence="ECO:0000250"
FT REGION 35..164
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 192..225
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 543..910
FT /note="Interaction with LPXN"
FT /evidence="ECO:0000250|UniProtKB:P10275"
FT REGION 563..653
FT /note="Interaction with HIPK3"
FT /evidence="ECO:0000250|UniProtKB:P15207"
FT REGION 583..910
FT /note="Interaction with CCAR1"
FT /evidence="ECO:0000250|UniProtKB:P10275"
FT REGION 616..910
FT /note="Interaction with KAT7"
FT /evidence="ECO:0000250|UniProtKB:P10275"
FT COMPBIAS 52..93
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 697
FT /ligand="17beta-hydroxy-5alpha-androstan-3-one"
FT /ligand_id="ChEBI:CHEBI:16330"
FT /evidence="ECO:0000269|PubMed:15328534,
FT ECO:0007744|PDB:1T73, ECO:0007744|PDB:1T74,
FT ECO:0007744|PDB:1T76, ECO:0007744|PDB:1T79,
FT ECO:0007744|PDB:1T7F, ECO:0007744|PDB:1T7M,
FT ECO:0007744|PDB:1T7R, ECO:0007744|PDB:1T7T"
FT BINDING 744
FT /ligand="17beta-hydroxy-5alpha-androstan-3-one"
FT /ligand_id="ChEBI:CHEBI:16330"
FT /evidence="ECO:0000269|PubMed:15328534,
FT ECO:0007744|PDB:1T73, ECO:0007744|PDB:1T74,
FT ECO:0007744|PDB:1T76, ECO:0007744|PDB:1T79,
FT ECO:0007744|PDB:1T7F, ECO:0007744|PDB:1T7M,
FT ECO:0007744|PDB:1T7R, ECO:0007744|PDB:1T7T"
FT BINDING 869
FT /ligand="17beta-hydroxy-5alpha-androstan-3-one"
FT /ligand_id="ChEBI:CHEBI:16330"
FT /evidence="ECO:0000269|PubMed:15328534,
FT ECO:0007744|PDB:1T73, ECO:0007744|PDB:1T74,
FT ECO:0007744|PDB:1T76, ECO:0007744|PDB:1T79,
FT ECO:0007744|PDB:1T7F, ECO:0007744|PDB:1T7M,
FT ECO:0007744|PDB:1T7R, ECO:0007744|PDB:1T7T"
FT SITE 712
FT /note="Interaction with coactivator LXXL and FXXFY motifs"
FT /evidence="ECO:0000269|PubMed:15328534"
FT SITE 889
FT /note="Interaction with coactivator FXXLF and FXXFY motifs"
FT /evidence="ECO:0000269|PubMed:15328534"
FT MOD_RES 81
FT /note="Phosphoserine; by CDK9"
FT /evidence="ECO:0000250|UniProtKB:P10275"
FT MOD_RES 93
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P10275"
FT MOD_RES 222
FT /note="Phosphotyrosine; by CSK"
FT /evidence="ECO:0000250|UniProtKB:P10275"
FT MOD_RES 255
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P10275"
FT MOD_RES 266
FT /note="Phosphotyrosine; by CSK and TNK2"
FT /evidence="ECO:0000250|UniProtKB:P10275"
FT MOD_RES 306
FT /note="Phosphotyrosine; by CSK"
FT /evidence="ECO:0000250|UniProtKB:P10275"
FT MOD_RES 345
FT /note="Phosphotyrosine; by CSK"
FT /evidence="ECO:0000250|UniProtKB:P10275"
FT MOD_RES 356
FT /note="Phosphotyrosine; by CSK"
FT /evidence="ECO:0000250|UniProtKB:P10275"
FT MOD_RES 361
FT /note="Phosphotyrosine; by CSK"
FT /evidence="ECO:0000250|UniProtKB:P10275"
FT MOD_RES 362
FT /note="Phosphotyrosine; by CSK and TNK2"
FT /evidence="ECO:0000250|UniProtKB:P10275"
FT MOD_RES 392
FT /note="Phosphotyrosine; by CSK"
FT /evidence="ECO:0000250|UniProtKB:P10275"
FT MOD_RES 526
FT /note="Phosphotyrosine; by CSK"
FT /evidence="ECO:0000250|UniProtKB:P10275"
FT MOD_RES 543
FT /note="Phosphotyrosine; by CSK"
FT /evidence="ECO:0000250|UniProtKB:P10275"
FT MOD_RES 642
FT /note="Phosphoserine; by STK4/MST1"
FT /evidence="ECO:0000250|UniProtKB:P10275"
FT MOD_RES 907
FT /note="Phosphotyrosine; by CSK"
FT /evidence="ECO:0000250|UniProtKB:P10275"
FT CROSSLNK 385
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO)"
FT /evidence="ECO:0000250"
FT CROSSLNK 512
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO)"
FT /evidence="ECO:0000250"
FT CROSSLNK 837
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0000250|UniProtKB:P10275"
FT CROSSLNK 839
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0000250|UniProtKB:P10275"
FT HELIX 664..672
FT /evidence="ECO:0007829|PDB:1T7R"
FT HELIX 689..712
FT /evidence="ECO:0007829|PDB:1T7R"
FT HELIX 717..719
FT /evidence="ECO:0007829|PDB:1T7R"
FT HELIX 722..749
FT /evidence="ECO:0007829|PDB:1T7R"
FT STRAND 752..757
FT /evidence="ECO:0007829|PDB:1T7R"
FT STRAND 760..762
FT /evidence="ECO:0007829|PDB:1T7R"
FT HELIX 764..769
FT /evidence="ECO:0007829|PDB:1T7R"
FT HELIX 773..788
FT /evidence="ECO:0007829|PDB:1T7R"
FT HELIX 793..804
FT /evidence="ECO:0007829|PDB:1T7R"
FT STRAND 806..809
FT /evidence="ECO:0007829|PDB:1T7R"
FT HELIX 816..835
FT /evidence="ECO:0007829|PDB:1T7R"
FT HELIX 841..874
FT /evidence="ECO:0007829|PDB:1T7R"
FT HELIX 876..879
FT /evidence="ECO:0007829|PDB:1T7R"
FT HELIX 885..893
FT /evidence="ECO:0007829|PDB:1T7R"
FT HELIX 895..899
FT /evidence="ECO:0007829|PDB:1T7R"
FT STRAND 902..905
FT /evidence="ECO:0007829|PDB:1T7R"
SQ SEQUENCE 911 AA; 98403 MW; 601B9BD4E697DAA4 CRC64;
MEVQLGLGRV YPRPPSKTYR GAFQNLFQSV REVIQNPGPR HPEAASAAPP GASLLLQQQQ
QQQQQQQQQQ QQQQQQQQET SPRQQQQQGE DGSPQAHRRG PTGYLVLDEE QQPSQPQSAP
ECHPERGCVP EPGAAVAASK GLPQQLPAPP DEDDSAAPST LSLLGPTFPG LSSCSADLKD
ILSEASTMQL LQQQQQEAVS EGSSSGRARE ASGAPTSSKD NYLGGTSTIS DSAKELCKAV
SVSMGLGVEA LEHLSPGEQL RGDCMYAPLL GVPPAVRPTP CAPLAECKGS LLDDSAGKST
EDTAEYSPFK GGYTKGLEGE SLGCSGSAAA GSSGTLELPS TLSLYKSGAL DEAAAYQSRD
YYNFPLALAG PPPPPPPPHP HARIKLENPL DYGSAWAAAA AQCRYGDLAS LHGAGAAGPG
SGSPSAAASS SWHTLFTAEE GQLYGPCGGG GGGGGGGGGG GGGGEAGAVA PYGYTRPPQG
LAGQEGDFTA PDVWYPGGMV SRVPYPSPTC VKSEMGPWMD SYSGPYGDMR LETARDHVLP
IDYYFPPQKT CLICGDEASG CHYGALTCGS CKVFFKRAAE GKQKYLCASR NDCTIDKFRR
KNCPSCRLRK CYEAGMTLGA RKLKKLGNLK LQEEGEASST TSPTEETTQK LTVSHIEGYE
CQPIFLNVLE AIEPGVVCAG HDNNQPDSFA ALLSSLNELG ERQLVHVVKW AKALPGFRNL
HVDDQMAVIQ YSWMGLMVFA MGWRSFTNVN SRMLYFAPDL VFNEYRMHKS RMYSQCVRMR
HLSQEFGWLQ ITPQEFLCMK ALLLFSIIPV DGLKNQKFFD ELRMNYIKEL DRIIACKRKN
PTSCSRRFYQ LTKLLDSVQP IARELHQFTF DLLIKSHMVS VDFPEMMAEI ISVQVPKILS
GKVKPIYFHT Q
