HCAR_ECOLI
ID HCAR_ECOLI Reviewed; 296 AA.
AC Q47141; P76583; P77167;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Hca operon transcriptional activator HcaR;
GN Name=hcaR; Synonyms=phdR, yfhT; OrderedLocusNames=b2537, JW2521;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RA Turlin E., Gasser F., Biville F.;
RL Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP CHARACTERIZATION.
RC STRAIN=K12 / MC1061 / ATCC 53338 / DSM 7140;
RX PubMed=9603882; DOI=10.1128/jb.180.11.2915-2923.1998;
RA Diaz E., Ferrandez A., Garcia J.L.;
RT "Characterization of the hca cluster encoding the dioxygenolytic pathway
RT for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-
RT 12.";
RL J. Bacteriol. 180:2915-2923(1998).
RN [6]
RP CAUTION.
RC STRAIN=K12 / MG1655 / ATCC 29425;
RX PubMed=22161628; DOI=10.1002/bit.24398;
RA Warnecke T.E., Lynch M.D., Lipscomb M.L., Gill R.T.;
RT "Identification of a 21 amino acid peptide conferring 3-hydroxypropionic
RT acid stress-tolerance to Escherichia coli.";
RL Biotechnol. Bioeng. 109:1347-1352(2012).
CC -!- FUNCTION: Transcriptional activator of the hca operon for 3-
CC phenylpropionic acid catabolism.
CC -!- SIMILARITY: Belongs to the LysR transcriptional regulatory family.
CC {ECO:0000305}.
CC -!- CAUTION: The 21 C-terminal amino acids have been proposed to be
CC expressed from an independent promoter within this gene and called
CC IroK. Overexpression of IroK has been suggested to be responsible for
CC 3-hydroxypropionic acid (3-HP) resistance. However the IroK peptide's
CC existence has not been proven, nor has mutagenesis of HcaR itself
CC rather than overexpression of IroK been ruled out as the cause of 3-HP
CC resistance (PubMed:22161628). {ECO:0000305|PubMed:22161628}.
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DR EMBL; Z37966; CAA86020.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75590.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA16432.2; -; Genomic_DNA.
DR PIR; H65030; H65030.
DR RefSeq; NP_417032.1; NC_000913.3.
DR RefSeq; WP_000423261.1; NZ_STEB01000011.1.
DR AlphaFoldDB; Q47141; -.
DR SMR; Q47141; -.
DR BioGRID; 4261574; 17.
DR IntAct; Q47141; 2.
DR STRING; 511145.b2537; -.
DR PaxDb; Q47141; -.
DR PRIDE; Q47141; -.
DR EnsemblBacteria; AAC75590; AAC75590; b2537.
DR EnsemblBacteria; BAA16432; BAA16432; BAA16432.
DR GeneID; 947000; -.
DR KEGG; ecj:JW2521; -.
DR KEGG; eco:b2537; -.
DR PATRIC; fig|1411691.4.peg.4197; -.
DR EchoBASE; EB3228; -.
DR eggNOG; COG0583; Bacteria.
DR HOGENOM; CLU_039613_6_4_6; -.
DR InParanoid; Q47141; -.
DR OMA; MESMEDH; -.
DR PhylomeDB; Q47141; -.
DR BioCyc; EcoCyc:G7331-MON; -.
DR PRO; PR:Q47141; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0032993; C:protein-DNA complex; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IDA:EcoliWiki.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:EcoCyc.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:EcoCyc.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:EcoCyc.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR005119; LysR_subst-bd.
DR InterPro; IPR000847; Tscrpt_reg_HTH_LysR.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00126; HTH_1; 1.
DR Pfam; PF03466; LysR_substrate; 1.
DR PRINTS; PR00039; HTHLYSR.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS50931; HTH_LYSR; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..296
FT /note="Hca operon transcriptional activator HcaR"
FT /id="PRO_0000105631"
FT DOMAIN 1..58
FT /note="HTH lysR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT DNA_BIND 18..38
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT CONFLICT 127..141
FT /note="ITTQQEEKIRRGELD -> SPRNRRKKFAVVNST (in Ref. 1;
FT CAA86020)"
FT /evidence="ECO:0000305"
FT CONFLICT 145..148
FT /note="MRHP -> NCAIH (in Ref. 1; CAA86020)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 296 AA; 32838 MW; 72FF08A62D490AAA CRC64;
MELRHLRYFV AVAQALNFTR AAEKLHTSQP SLSSQIRDLE NCVGVPLLVR DKRKVALTAA
GECFLQDALA ILEQAENAKL RARKIVQEDR QLTIGFVPSA EVNLLPKVLP MFRLRQPDTL
IELVSLITTQ QEEKIRRGEL DVGLMRHPVY SPEIDYLELF DEPLVVVLPV DHPLAHEKEI
TAAQLDGVNF VSTDPVYSGS LAPIVKAWFA QENSQPNIVQ VATNILVTMN LVGMGLGVTL
IPGYMNNFNT GQVVFRPIAG NVPSIALLMA WKKGEMKPAL RDFIAIVQER LASVTA
