ID   HCAR_ORYSJ              Reviewed;         471 AA.
AC   Q7XTG7; Q0JE66;
DT   22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2004, sequence version 2.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=7-hydroxymethyl chlorophyll a reductase, chloroplastic;
DE            EC=1.17.7.2 {ECO:0000250|UniProtKB:Q8GS60};
DE   Flags: Precursor;
GN   Name=HCAR; OrderedLocusNames=Os04g0320100, LOC_Os04g25400;
GN   ORFNames=OSJNBa0041M06.1, OSJNBb0026L04.9;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447439; DOI=10.1038/nature01183;
RA   Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA   Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA   Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA   Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA   Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA   Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA   Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA   Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA   Li J., Hong G., Xue Y., Han B.;
RT   "Sequence and analysis of rice chromosome 4.";
RL   Nature 420:316-320(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
CC   -!- FUNCTION: Probable iron-sulfur flavoprotein that converts 7-
CC       hydroxymethyl chlorophyll a to chlorophyll a using ferredoxin as a
CC       reducing equivalent. Catalyzes the reduction of a hydroxymethyl group
CC       to a methyl group. {ECO:0000250|UniProtKB:Q8GS60}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=chlorophyll a + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = 7(1)-
CC         hydroxychlorophyll a + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin];
CC         Xref=Rhea:RHEA:53544, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33737,
CC         ChEBI:CHEBI:33738, ChEBI:CHEBI:58416, ChEBI:CHEBI:83377; EC=1.17.7.2;
CC         Evidence={ECO:0000250|UniProtKB:Q8GS60};
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000250|UniProtKB:Q8GS60};
CC   -!- COFACTOR:
CC       Name=iron-sulfur cluster; Xref=ChEBI:CHEBI:30408;
CC         Evidence={ECO:0000250|UniProtKB:Q8GS60};
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC       {ECO:0000250|UniProtKB:Q8GS60}.
CC   -!- SIMILARITY: Belongs to the FrhB family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAF14371.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AL606443; CAE01504.2; -; Genomic_DNA.
DR   EMBL; AL663021; CAE04699.2; -; Genomic_DNA.
DR   EMBL; AP008210; BAF14371.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP014960; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; XP_015636785.1; XM_015781299.1.
DR   AlphaFoldDB; Q7XTG7; -.
DR   SMR; Q7XTG7; -.
DR   STRING; 4530.OS04T0320100-01; -.
DR   CAZy; GT1; Glycosyltransferase Family 1.
DR   PaxDb; Q7XTG7; -.
DR   PRIDE; Q7XTG7; -.
DR   EnsemblPlants; Os04t0320100-02; Os04t0320100-02; Os04g0320100.
DR   GeneID; 4335472; -.
DR   Gramene; Os04t0320100-02; Os04t0320100-02; Os04g0320100.
DR   KEGG; osa:4335472; -.
DR   eggNOG; ENOG502QR65; Eukaryota.
DR   HOGENOM; CLU_850978_0_0_1; -.
DR   InParanoid; Q7XTG7; -.
DR   OrthoDB; 788166at2759; -.
DR   Proteomes; UP000000763; Chromosome 4.
DR   Proteomes; UP000059680; Chromosome 4.
DR   ExpressionAtlas; Q7XTG7; baseline and differential.
DR   Genevisible; Q7XTG7; OS.
DR   GO; GO:0009507; C:chloroplast; IBA:GO_Central.
DR   GO; GO:0090415; F:7-hydroxymethyl chlorophyll a reductase activity; IBA:GO_Central.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0052592; F:oxidoreductase activity, acting on CH or CH2 groups, with an iron-sulfur protein as acceptor; IBA:GO_Central.
DR   GO; GO:0033354; P:chlorophyll cycle; IBA:GO_Central.
DR   InterPro; IPR007516; Co_F420_Hydgase/DH_bsu_N.
DR   InterPro; IPR045220; FRHB/FDHB/HCAR-like.
DR   InterPro; IPR007525; FrhB_FdhB_C.
DR   InterPro; IPR011254; Prismane-like_sf.
DR   PANTHER; PTHR31332; PTHR31332; 1.
DR   Pfam; PF04432; FrhB_FdhB_C; 1.
DR   Pfam; PF04422; FrhB_FdhB_N; 1.
DR   SUPFAM; SSF56821; SSF56821; 1.
PE   3: Inferred from homology;
KW   Chloroplast; FAD; Flavoprotein; Iron; Iron-sulfur; Metal-binding;
KW   Oxidoreductase; Plastid; Reference proteome; Transit peptide.
FT   TRANSIT         1..44
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           45..471
FT                   /note="7-hydroxymethyl chlorophyll a reductase,
FT                   chloroplastic"
FT                   /id="PRO_0000415616"
SQ   SEQUENCE   471 AA;  51982 MW;  653519A187F4C10E CRC64;
     MARCISFLST SSSLPCATKP PCCSVSSVLP SSPSSHQCRG RKTSCGSIRA LREDWRERSK
     AIPPGGVYPA KDHCSQCGLC DTYYIAHVKN ACAFLGDGMS RVEDLEPLVH GRGRKQDMDE
     MYFGVYEQLL YARKMKPVEG AQWTGIVTTI AVEMLKANMV DAVVCVQSDP DDRLAPMPVL
     ARTPDEVIAA KGVKPTLSPN LNTLALVEAA GVKRLLFCGV GCQVQALRSV EKYLGLEKLY
     VLGTNCVDNG TREGLDKFLK AASSEPETVL HYEFMQDYKV HLKHLDGHIE EVPYFCLPAK
     DLVDVIAPSC YSCFDYTNGL ADLVVGYMGV PKYPGVSMTQ HPQYITVRND RGREMLSLVE
     GLLESTPTVS SGVRQPFVIE TVKADDEAKQ GRGPSQPAPT FVGNVIAFLL NLIGPKGLEF
     ARYSLDYHTI RNYLHVNRAW GKQRAEQHIP SYAKKIVEAY DKDGRIESML Q