HCAT_ECOLI
ID HCAT_ECOLI Reviewed; 379 AA.
AC Q47142; O05660; P77638;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Probable 3-phenylpropionic acid transporter;
GN Name=hcaT; Synonyms=yfhS; OrderedLocusNames=b2536, JW2520;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RA Turlin E., Gasser F., Biville F.;
RL Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RC STRAIN=K12 / MC1061 / ATCC 53338 / DSM 7140;
RX PubMed=9603882; DOI=10.1128/jb.180.11.2915-2923.1998;
RA Diaz E., Ferrandez A., Garcia J.L.;
RT "Characterization of the hca cluster encoding the dioxygenolytic pathway
RT for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-
RT 12.";
RL J. Bacteriol. 180:2915-2923(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- FUNCTION: Probable permease involved in the uptake of 3-phenylpropionic
CC acid.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Phenyl
CC propionate permease (PPP) (TC 2.A.1.27) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA86021.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; Z37966; CAA86021.1; ALT_FRAME; Genomic_DNA.
DR EMBL; Y11071; CAA71954.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75589.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA16431.1; -; Genomic_DNA.
DR PIR; G65030; G65030.
DR PIR; S49295; S49295.
DR RefSeq; NP_417031.1; NC_000913.3.
DR RefSeq; WP_000244193.1; NZ_LN832404.1.
DR AlphaFoldDB; Q47142; -.
DR SMR; Q47142; -.
DR BioGRID; 4261387; 11.
DR STRING; 511145.b2536; -.
DR TCDB; 2.A.1.27.1; the major facilitator superfamily (mfs).
DR PaxDb; Q47142; -.
DR PRIDE; Q47142; -.
DR EnsemblBacteria; AAC75589; AAC75589; b2536.
DR EnsemblBacteria; BAA16431; BAA16431; BAA16431.
DR GeneID; 66673576; -.
DR GeneID; 947007; -.
DR KEGG; ecj:JW2520; -.
DR KEGG; eco:b2536; -.
DR PATRIC; fig|1411691.4.peg.4198; -.
DR EchoBASE; EB3227; -.
DR eggNOG; COG2814; Bacteria.
DR HOGENOM; CLU_013133_6_0_6; -.
DR InParanoid; Q47142; -.
DR OMA; HGPYYTF; -.
DR PhylomeDB; Q47142; -.
DR BioCyc; EcoCyc:HCAT-MON; -.
DR PRO; PR:Q47142; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0030395; F:lactose binding; IBA:GO_Central.
DR GO; GO:0015528; F:lactose:proton symporter activity; IBA:GO_Central.
DR GO; GO:0071702; P:organic substance transport; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR026032; HcaT.
DR InterPro; IPR024989; MFS_assoc_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF12832; MFS_1_like; 1.
DR PIRSF; PIRSF004925; HcaT; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00902; 2A0127; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..379
FT /note="Probable 3-phenylpropionic acid transporter"
FT /id="PRO_0000196189"
FT TOPO_DOM 1..4
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 5..31
FT /note="Helical; Name=1"
FT /evidence="ECO:0000250"
FT TOPO_DOM 32..37
FT /note="Periplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 38..66
FT /note="Helical; Name=2"
FT /evidence="ECO:0000250"
FT TOPO_DOM 67..70
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 71..96
FT /note="Helical; Name=3"
FT /evidence="ECO:0000250"
FT TOPO_DOM 97..100
FT /note="Periplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 101..118
FT /note="Helical; Name=4"
FT /evidence="ECO:0000250"
FT TOPO_DOM 119..129
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 130..152
FT /note="Helical; Name=5"
FT /evidence="ECO:0000250"
FT TOPO_DOM 153..155
FT /note="Periplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 156..175
FT /note="Helical; Name=6"
FT /evidence="ECO:0000250"
FT TOPO_DOM 176..207
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 208..227
FT /note="Helical; Name=7"
FT /evidence="ECO:0000250"
FT TOPO_DOM 228..231
FT /note="Periplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 232..256
FT /note="Helical; Name=8"
FT /evidence="ECO:0000250"
FT TOPO_DOM 257..266
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 267..286
FT /note="Helical; Name=9"
FT /evidence="ECO:0000250"
FT TOPO_DOM 287..289
FT /note="Periplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 290..312
FT /note="Helical; Name=10"
FT /evidence="ECO:0000250"
FT TOPO_DOM 313..323
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 324..351
FT /note="Helical; Name=11"
FT /evidence="ECO:0000250"
FT TOPO_DOM 352..354
FT /note="Periplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 355..375
FT /note="Helical; Name=12"
FT /evidence="ECO:0000250"
FT TOPO_DOM 376..379
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT CONFLICT 191
FT /note="S -> N (in Ref. 2; CAA71954)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 379 AA; 41593 MW; 76429AD807623482 CRC64;
MVLQSTRWLA LGYFTYFFSY GIFLPFWSVW LKGIGLTPET IGLLLGAGLV ARFLGSLLIA
PRVSDPSRLI SALRVLALLT LLFAVAFWAG AHVAWLMLVM IGFNLFFSPL VPLTDALANT
WQKQFPLDYG KVRLWGSVAF VIGSALTGKL VTMFDYRVIL ALLTLGVASM LLGFLIRPTI
QPQGASRQQE STGWSAWLAL VRQNWRFLAC VCLLQGAHAA YYGFSAIYWQ AAGYSASAVG
YLWSLGVVAE VIIFALSNKL FRRCSARDML LISAICGVVR WGIMGATTAL PWLIVVQILH
CGTFTVCHLA AMRYIAARQG SEVIRLQAVY SAVAMGGSIA IMTVFAGFLY QYLGHGVFWV
MALVALPAMF LRPKVVPSC
