ID   HCBT1_DIACA             Reviewed;         445 AA.
AC   O24645;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=Anthranilate N-benzoyltransferase protein 1;
DE            EC=2.3.1.144;
DE   AltName: Full=Anthranilate N-hydroxycinnamoyl/benzoyltransferase 1;
GN   Name=HCBT1;
OS   Dianthus caryophyllus (Carnation) (Clove pink).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Caryophyllaceae; Caryophylleae; Dianthus.
OX   NCBI_TaxID=3570;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 7-18; 27-45; 74-84;
RP   197-230; 299-306; 343-350 AND 373-385, BIOPHYSICOCHEMICAL PROPERTIES,
RP   ENZYME ACTIVITY, AND FUNCTION.
RX   PubMed=9426598; DOI=10.1023/a:1005878622437;
RA   Yang Q., Reinhard K., Schiltz E., Matern U.;
RT   "Characterization and heterologous expression of hydroxycinnamoyl/benzoyl-
RT   CoA:anthranilate N-hydroxycinnamoyl/benzoyltransferase from elicited cell
RT   cultures of carnation, Dianthus caryophyllus L.";
RL   Plant Mol. Biol. 35:777-789(1997).
RN   [2]
RP   INDUCTION.
RX   PubMed=9869425; DOI=10.1023/a:1006003731919;
RA   Yang Q., Grimmig B., Matern U.;
RT   "Anthranilate N-hydroxycinnamoyl/benzoyltransferase gene from carnation:
RT   rapid elicitation of transcription and promoter analysis.";
RL   Plant Mol. Biol. 38:1201-1214(1998).
CC   -!- FUNCTION: Catalyzes the formation of N-benzoylanthranilate, in the
CC       course of methoxydianthramide B, a phytoalexin. Phytoalexins are
CC       produced in response to infection by parasites, and are essential for
CC       the expression of disease resistance. {ECO:0000269|PubMed:9426598}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=anthranilate + benzoyl-CoA = CoA + N-benzoylanthranilate;
CC         Xref=Rhea:RHEA:21600, ChEBI:CHEBI:16567, ChEBI:CHEBI:17331,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57369; EC=2.3.1.144;
CC         Evidence={ECO:0000269|PubMed:9426598};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Inactive below pH 7.0. {ECO:0000269|PubMed:9426598};
CC   -!- PATHWAY: Phytoalexin biosynthesis; methoxydianthramide B biosynthesis.
CC   -!- INDUCTION: By fungal elicitors. Elicitation triggers a rapid, transient
CC       induction, reaching maximal abundances within about 0.5 hours and
CC       returning to basal levels within 4 hours. {ECO:0000269|PubMed:9869425}.
CC   -!- PTM: N-terminus is blocked. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
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DR   EMBL; Z84383; CAB06427.1; -; mRNA.
DR   EMBL; Z84385; CAB06429.1; -; mRNA.
DR   PIR; T10717; T10717.
DR   AlphaFoldDB; O24645; -.
DR   SMR; O24645; -.
DR   KEGG; ag:CAB06427; -.
DR   BioCyc; MetaCyc:MON-15060; -.
DR   BRENDA; 2.3.1.144; 1925.
DR   UniPathway; UPA00900; -.
DR   GO; GO:0047672; F:anthranilate N-benzoyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.559.10; -; 2.
DR   InterPro; IPR023213; CAT-like_dom_sf.
PE   1: Evidence at protein level;
KW   Acyltransferase; Direct protein sequencing; Flavonoid biosynthesis;
KW   Transferase.
FT   CHAIN           1..445
FT                   /note="Anthranilate N-benzoyltransferase protein 1"
FT                   /id="PRO_0000147363"
FT   ACT_SITE        164
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        392
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   445 AA;  50038 MW;  1FEA06CA25D4CA8A CRC64;
     MSIQIKQSTM VRPAEETPNK SLWLSNIDMI LRTPYSHTGA VLIYKQPDNN EDNIHPSSSM
     YFDANILIEA LSKALVPFYP MAGRLKINGD RYEIDCNAEG ALFVEAESSH VLEDFGDFRP
     NDELHRVMVP TCDYSKGISS FPLLMVQLTR FRCGGVSIGF AQHHHVCDGM AHFEFNNSWA
     RIAKGLLPAL EPVHDRYLHL RPRNPPQIKY SHSQFEPFVP SLPNELLDGK TNKSQTLFIL
     SREQINTLKQ KLDLSNNTTR LSTYEVVAAH VWRSVSKARG LSDHEEIKLI MPVDGRSRIN
     NPSLPKGYCG NVVFLAVCTA TVGDLSCNPL TDTAGKVQEA LKGLDDDYLR SAIDHTESKP
     GLPVPYMGSP EKTLYPNVLV NSWGRIPYQA MDFGWGSPTF FGISNIFYDG QCFLIPSRDG
     DGSMTLAINL FSSHLSRFKK YFYDF