ID   HCBT2_DIACA             Reviewed;         446 AA.
AC   O23917;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 62.
DE   RecName: Full=Anthranilate N-benzoyltransferase protein 2;
DE            EC=2.3.1.144;
DE   AltName: Full=Anthranilate N-hydroxycinnamoyl/benzoyltransferase 2;
GN   Name=HCBT2;
OS   Dianthus caryophyllus (Carnation) (Clove pink).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Caryophyllaceae; Caryophylleae; Dianthus.
OX   NCBI_TaxID=3570;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 7-18; 27-45; 74-84;
RP   204-230; 300-307; 344-369 AND 374-386, AND FUNCTION.
RX   PubMed=9426598; DOI=10.1023/a:1005878622437;
RA   Yang Q., Reinhard K., Schiltz E., Matern U.;
RT   "Characterization and heterologous expression of hydroxycinnamoyl/benzoyl-
RT   CoA:anthranilate N-hydroxycinnamoyl/benzoyltransferase from elicited cell
RT   cultures of carnation, Dianthus caryophyllus L.";
RL   Plant Mol. Biol. 35:777-789(1997).
RN   [2]
RP   INDUCTION.
RX   PubMed=9869425; DOI=10.1023/a:1006003731919;
RA   Yang Q., Grimmig B., Matern U.;
RT   "Anthranilate N-hydroxycinnamoyl/benzoyltransferase gene from carnation:
RT   rapid elicitation of transcription and promoter analysis.";
RL   Plant Mol. Biol. 38:1201-1214(1998).
CC   -!- FUNCTION: Catalyzes the formation of N-benzoylanthranilate, in the
CC       course of methoxydianthramide B, a phytoalexin. Phytoalexins are
CC       produced in response to infection by parasites, and are essential for
CC       the expression of disease resistance. {ECO:0000269|PubMed:9426598}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=anthranilate + benzoyl-CoA = CoA + N-benzoylanthranilate;
CC         Xref=Rhea:RHEA:21600, ChEBI:CHEBI:16567, ChEBI:CHEBI:17331,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57369; EC=2.3.1.144;
CC   -!- PATHWAY: Phytoalexin biosynthesis; methoxydianthramide B biosynthesis.
CC   -!- INDUCTION: By fungal elicitors. Elicitation triggers a rapid, transient
CC       induction, reaching maximal abundances within about 0.5 hours and
CC       returning to basal levels within 4 hours. {ECO:0000269|PubMed:9869425}.
CC   -!- PTM: N-terminus is blocked. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
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DR   EMBL; Z84386; CAB06430.1; -; mRNA.
DR   EMBL; Z98758; CAB11466.1; -; Genomic_DNA.
DR   PIR; T10711; T10711.
DR   AlphaFoldDB; O23917; -.
DR   SMR; O23917; -.
DR   BioCyc; MetaCyc:MON-15061; -.
DR   UniPathway; UPA00900; -.
DR   GO; GO:0047672; F:anthranilate N-benzoyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.559.10; -; 2.
DR   InterPro; IPR023213; CAT-like_dom_sf.
PE   1: Evidence at protein level;
KW   Acyltransferase; Direct protein sequencing; Flavonoid biosynthesis;
KW   Transferase.
FT   CHAIN           1..446
FT                   /note="Anthranilate N-benzoyltransferase protein 2"
FT                   /id="PRO_0000147364"
FT   ACT_SITE        164
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        393
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   446 AA;  50147 MW;  E2C0269D83247C0C CRC64;
     MSIQIKQSTM VRPAEETPNK SLWLSKIDMI LRTPYSHTGA VLIYKQPDNN EDNIHPSSSM
     YFDANILIEA LSKALVPYYP MAGRLKINGD RYEIDCNAEG ALFVEAESSH VLEDFGDFRP
     NDELHRVMVP TCDYSKGISS FPLLMVQLTR FRCGGVSIGF AQHHHACDGM SHFEFNNSWA
     RIAKGLLPAL EPVHDRYLHL RLRNPPQIKY THSQFEPFVP SLPNELLDGK TNKSQTLFKL
     SREQINTLKQ KLDLSSNTTT RLSTYEVVAG HVWRSVSKAR GLSDHEEIKL IMPVDGRSRI
     NNPSLPKGYC GNVVFLAVCT ATVGDLSCNP LTDTAGKVQE ALKGLDDDYL RSAIDHTESK
     PDLPVPYMGS PEKTLYPNVL VNSWGRIPYQ AMDFGWGSPT FFGISNIFYD GQCFLIPSQN
     GDGSMTLAIN LFSSHLSLFK KYFYDF