HCN1_SCHPO
ID HCN1_SCHPO Reviewed; 80 AA.
AC O13916;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Anaphase-promoting complex subunit hcn1;
DE AltName: Full=20S cyclosome/APC complex protein hcn1;
DE AltName: Full=Chaperone-like protein hcn1;
DE AltName: Full=High copy suppressor of cut9 protein 1;
GN Name=hcn1; ORFNames=SPAC23C11.12;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX PubMed=9264466; DOI=10.1242/jcs.110.15.1793;
RA Yamada H., Kumada K., Yanagida M.;
RT "Distinct subunit functions and cell cycle regulated phosphorylation of 20S
RT APC/cyclosome required for anaphase in fission yeast.";
RL J. Cell Sci. 110:1793-1804(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP SUBUNIT.
RX PubMed=12477395; DOI=10.1016/s0960-9822(02)01331-3;
RA Yoon H.-J., Feoktistova A., Wolfe B.A., Jennings J.L., Link A.J.,
RA Gould K.L.;
RT "Proteomics analysis identifies new components of the fission and budding
RT yeast anaphase-promoting complexes.";
RL Curr. Biol. 12:2048-2054(2002).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) IN COMPLEX WITH CUT9, ACETYLATION AT
RP MET-1, AND SUBUNIT.
RX PubMed=20924356; DOI=10.1038/emboj.2010.247;
RA Zhang Z., Kulkarni K., Hanrahan S.J., Thompson A.J., Barford D.;
RT "The APC/C subunit Cdc16/Cut9 is a contiguous tetratricopeptide repeat
RT superhelix with a homo-dimer interface similar to Cdc27.";
RL EMBO J. 29:3733-3744(2010).
CC -!- FUNCTION: Component of the anaphase promoting complex/cyclosome
CC (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex
CC that controls progression through mitosis and the G1 phase of the cell
CC cycle. The APC/C is thought to confer substrate specificity and, in the
CC presence of ubiquitin-conjugating E2 enzymes, it catalyzes the
CC formation of protein-ubiquitin conjugates that are subsequently
CC degraded by the 26S proteasome. Has a role in assembling cut9 in the
CC 20S APC/cyclosome. {ECO:0000269|PubMed:9264466}.
CC -!- SUBUNIT: The APC/C is composed of at least 13 subunits: apc1, apc2,
CC nuc2, apc4, apc5, cut9, apc8, apc10, apc11, hcn1, apc13, apc14 and
CC apc15. Interacts directly (via N-terminus) with cut9.
CC {ECO:0000269|PubMed:12477395, ECO:0000269|PubMed:20924356}.
CC -!- INTERACTION:
CC O13916; P41889: cut9; NbExp=2; IntAct=EBI-1251563, EBI-1160859;
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DR EMBL; AB001373; BAD34487.1; -; Genomic_DNA.
DR EMBL; CU329670; CAB11170.1; -; Genomic_DNA.
DR PIR; T38250; T38250.
DR RefSeq; NP_593643.1; NM_001019074.1.
DR PDB; 2XPI; X-ray; 2.60 A; B/E=1-80.
DR PDBsum; 2XPI; -.
DR AlphaFoldDB; O13916; -.
DR SMR; O13916; -.
DR BioGRID; 278526; 5.
DR ComplexPortal; CPX-763; Anaphase-Promoting Complex variant 1.
DR ComplexPortal; CPX-764; Anaphase-Promoting Complex variant 2.
DR ComplexPortal; CPX-765; Anaphase-Promoting Complex variant 3.
DR ComplexPortal; CPX-766; Anaphase-Promoting Complex variant 4.
DR IntAct; O13916; 3.
DR MINT; O13916; -.
DR STRING; 4896.SPAC23C11.12.1; -.
DR iPTMnet; O13916; -.
DR MaxQB; O13916; -.
DR PaxDb; O13916; -.
DR PRIDE; O13916; -.
DR EnsemblFungi; SPAC23C11.12.1; SPAC23C11.12.1:pep; SPAC23C11.12.
DR GeneID; 2542045; -.
DR KEGG; spo:SPAC23C11.12; -.
DR PomBase; SPAC23C11.12; hcn1.
DR VEuPathDB; FungiDB:SPAC23C11.12; -.
DR HOGENOM; CLU_2723626_0_0_1; -.
DR InParanoid; O13916; -.
DR OMA; GITQIFD; -.
DR PRO; PR:O13916; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005680; C:anaphase-promoting complex; IDA:PomBase.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0031145; P:anaphase-promoting complex-dependent catabolic process; IEA:InterPro.
DR GO; GO:0031536; P:positive regulation of exit from mitosis; IMP:PomBase.
DR GO; GO:0030071; P:regulation of mitotic metaphase/anaphase transition; IEA:InterPro.
DR DisProt; DP01246; -.
DR InterPro; IPR018860; APC_suCDC26.
DR Pfam; PF10471; ANAPC_CDC26; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Chaperone; Reference proteome;
KW Ubl conjugation pathway.
FT CHAIN 1..80
FT /note="Anaphase-promoting complex subunit hcn1"
FT /id="PRO_0000083923"
FT REGION 26..54
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000269|PubMed:20924356"
FT HELIX 13..20
FT /evidence="ECO:0007829|PDB:2XPI"
SQ SEQUENCE 80 AA; 9074 MW; 9402F00563878B29 CRC64;
MLRRNPTAIQ ITAEDVLAYD EEKLRQTLDS ESTTEEALQK NEESTRLSPE KKKIIRERRI
GITQIFDSSM HPSQGGAAQS
