HCP15_BPSPP
ID HCP15_BPSPP Reviewed; 102 AA.
AC Q38584;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 29-SEP-2021, entry version 76.
DE RecName: Full=Head completion protein gp15 {ECO:0000305};
DE Short=HCP;
DE AltName: Full=Connector protein gp15 {ECO:0000312|EMBL:CAA61872.1};
DE AltName: Full=Gene product 15;
DE Short=Gp15;
GN Name=15 {ECO:0000312|EMBL:CAA61872.1};
OS Bacillus phage SPP1 (Bacteriophage SPP1).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Siphoviridae.
OX NCBI_TaxID=10724;
OH NCBI_TaxID=1423; Bacillus subtilis.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9180375; DOI=10.1006/jmbi.1997.0997;
RA Becker B., de la Fuente N., Gassel M., Guenther D., Tavares P., Lurz R.,
RA Trautner T.A., Alonso J.C.;
RT "Head morphogenesis genes of the Bacillus subtilis bacteriophage SPP1.";
RL J. Mol. Biol. 268:822-839(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=9434185; DOI=10.1016/s0378-1119(97)00547-7;
RA Alonso J.C., Luder G., Stiege A.C., Chai S., Weise F., Trautner T.A.;
RT "The complete nucleotide sequence and functional organization of Bacillus
RT subtilis bacteriophage SPP1.";
RL Gene 204:201-212(1997).
RN [3] {ECO:0007744|PDB:2KBZ}
RP STRUCTURE BY NMR OF 4-102.
RX PubMed=19433794; DOI=10.1073/pnas.0812407106;
RA Lhuillier S., Gallopin M., Gilquin B., Brasiles S., Lancelot N.,
RA Letellier G., Gilles M., Dethan G., Orlova E.V., Couprie J., Tavares P.,
RA Zinn-Justin S.;
RT "Structure of bacteriophage SPP1 head-to-tail connection reveals mechanism
RT for viral DNA gating.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:8507-8512(2009).
RN [4] {ECO:0007744|PDB:5A20, ECO:0007744|PDB:5A21}
RP STRUCTURE BY ELECTRON MICROSCOPY (7.20 ANGSTROMS), INTERACTION WITH THE
RP STOPPER GP16, INTERACTION WITH THE PORTAL PROTEIN, SUBUNIT, SUBCELLULAR
RP LOCATION, AND FUNCTION.
RX PubMed=25991862; DOI=10.1073/pnas.1504039112;
RA Chaban Y., Lurz R., Brasiles S., Cornilleau C., Karreman M.,
RA Zinn-Justin S., Tavares P., Orlova E.V.;
RT "Structural rearrangements in the phage head-to-tail interface during
RT assembly and infection.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:7009-7014(2015).
CC -!- FUNCTION: Head completion protein that exhibits an open central channel
CC for viral DNA ejection. Part of the head-tail connector by binding to
CC the portal protein and to the head completion protein 16.
CC {ECO:0000305|PubMed:25991862}.
CC -!- SUBUNIT: Homododecamer. Interacts with the stopper protein gp16.
CC Interacts with the portal protein; this interaction occurs at the end
CC of the packaging when the terminase complex is replaced by the
CC connector. {ECO:0000269|PubMed:25991862}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:25991862}. Note=Part
CC of the connector between the portal and the tail.
CC {ECO:0000269|PubMed:25991862}.
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DR EMBL; X89721; CAA61872.1; -; Genomic_DNA.
DR EMBL; X97918; CAA66546.1; -; Genomic_DNA.
DR PIR; S58144; S58144.
DR RefSeq; NP_690676.1; NC_004166.2.
DR PDB; 2KBZ; NMR; -; A=4-102.
DR PDB; 5A20; EM; 7.60 A; C/D=1-102.
DR PDB; 5A21; EM; 7.20 A; C/D=1-102.
DR PDBsum; 2KBZ; -.
DR PDBsum; 5A20; -.
DR PDBsum; 5A21; -.
DR SMR; Q38584; -.
DR GeneID; 955305; -.
DR KEGG; vg:955305; -.
DR EvolutionaryTrace; Q38584; -.
DR Proteomes; UP000002559; Genome.
DR GO; GO:0099001; P:viral genome ejection through host cell envelope, long flexible tail mechanism; IEA:UniProtKB-KW.
DR InterPro; IPR021146; Phafe_gp6_head-tail.
DR Pfam; PF05135; Phage_connect_1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome;
KW Viral genome ejection through host cell envelope;
KW Viral long flexible tail ejection system;
KW Viral penetration into host cytoplasm; Virion; Virus entry into host cell.
FT CHAIN 1..102
FT /note="Head completion protein gp15"
FT /id="PRO_0000438140"
FT HELIX 7..10
FT /evidence="ECO:0007829|PDB:2KBZ"
FT TURN 11..17
FT /evidence="ECO:0007829|PDB:2KBZ"
FT HELIX 18..33
FT /evidence="ECO:0007829|PDB:2KBZ"
FT TURN 34..36
FT /evidence="ECO:0007829|PDB:2KBZ"
FT HELIX 51..68
FT /evidence="ECO:0007829|PDB:2KBZ"
FT STRAND 76..79
FT /evidence="ECO:0007829|PDB:2KBZ"
FT TURN 82..84
FT /evidence="ECO:0007829|PDB:2KBZ"
FT HELIX 90..92
FT /evidence="ECO:0007829|PDB:2KBZ"
FT TURN 96..99
FT /evidence="ECO:0007829|PDB:2KBZ"
SQ SEQUENCE 102 AA; 11614 MW; 3B7B0FFC48D6C025 CRC64;
MDIQRVKRLL SITNDKHDEY LTEMVPLLVE FAKDECHNPF IDKDGNESIP SGVLIFVAKA
AQFYMTNAGL TGRSMDTVSY NFATEIPSTI LKKLNPYRKM AR
