HCP1_PSEAE
ID HCP1_PSEAE Reviewed; 162 AA.
AC Q9I747;
DT 03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Protein hcp1;
GN Name=hcp1; OrderedLocusNames=PA0085;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS), SUBUNIT, SUBCELLULAR LOCATION, AND
RP INDUCTION.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=16763151; DOI=10.1126/science.1128393;
RA Mougous J.D., Cuff M.E., Raunser S., Shen A., Zhou M., Gifford C.A.,
RA Goodman A.L., Joachimiak G., Ordonez C.L., Lory S., Walz T., Joachimiak A.,
RA Mekalanos J.J.;
RT "A virulence locus of Pseudomonas aeruginosa encodes a protein secretion
RT apparatus.";
RL Science 312:1526-1530(2006).
CC -!- FUNCTION: Required for assembly of the protein secretion apparatus HSI-
CC I. Actively secreted during chronic infection of cystic fibrosis
CC patients.
CC -!- SUBUNIT: Hexamer. Three hcp1 monomers form two closely related
CC hexameric rings with a 40 Angstrom internal diameter.
CC {ECO:0000269|PubMed:16763151}.
CC -!- INTERACTION:
CC Q9I747; Q9I747: hcp1; NbExp=3; IntAct=EBI-15689388, EBI-15689388;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16763151}.
CC -!- INDUCTION: Transcriptionally repressed by RetS and activated by LadS.
CC {ECO:0000269|PubMed:16763151}.
CC -!- MISCELLANEOUS: ATP hydrolysis by the first AAA domain of ClpV1 is
CC required for Hcp1 secretion.
CC -!- SIMILARITY: Belongs to the hcp1 family. {ECO:0000305}.
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DR EMBL; AE004091; AAG03475.1; -; Genomic_DNA.
DR PIR; B83635; B83635.
DR RefSeq; NP_248775.1; NC_002516.2.
DR RefSeq; WP_003083670.1; NZ_QZGE01000015.1.
DR PDB; 1Y12; X-ray; 1.95 A; A/B/C=1-162.
DR PDB; 6AP1; EM; 3.20 A; A/B/C/D/E/F=1-162.
DR PDBsum; 1Y12; -.
DR PDBsum; 6AP1; -.
DR AlphaFoldDB; Q9I747; -.
DR SMR; Q9I747; -.
DR DIP; DIP-29826N; -.
DR STRING; 287.DR97_3042; -.
DR PaxDb; Q9I747; -.
DR PRIDE; Q9I747; -.
DR DNASU; 879446; -.
DR EnsemblBacteria; AAG03475; AAG03475; PA0085.
DR GeneID; 879446; -.
DR KEGG; pae:PA0085; -.
DR PATRIC; fig|208964.12.peg.89; -.
DR PseudoCAP; PA0085; -.
DR HOGENOM; CLU_112762_0_3_6; -.
DR InParanoid; Q9I747; -.
DR OMA; NLMKYCL; -.
DR PhylomeDB; Q9I747; -.
DR BioCyc; PAER208964:G1FZ6-87-MON; -.
DR EvolutionaryTrace; Q9I747; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR Gene3D; 2.30.110.20; -; 1.
DR InterPro; IPR036624; Hcp1-lik_sf.
DR InterPro; IPR008514; T6SS_Hcp.
DR Pfam; PF05638; T6SS_HCP; 1.
DR SUPFAM; SSF141452; SSF141452; 1.
DR TIGRFAMs; TIGR03344; VI_effect_Hcp1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; Secreted.
FT CHAIN 1..162
FT /note="Protein hcp1"
FT /id="PRO_0000250698"
FT STRAND 4..9
FT /evidence="ECO:0007829|PDB:1Y12"
FT STRAND 19..21
FT /evidence="ECO:0007829|PDB:1Y12"
FT STRAND 24..36
FT /evidence="ECO:0007829|PDB:1Y12"
FT STRAND 51..53
FT /evidence="ECO:0007829|PDB:1Y12"
FT STRAND 56..62
FT /evidence="ECO:0007829|PDB:1Y12"
FT HELIX 66..75
FT /evidence="ECO:0007829|PDB:1Y12"
FT STRAND 79..87
FT /evidence="ECO:0007829|PDB:1Y12"
FT STRAND 98..111
FT /evidence="ECO:0007829|PDB:1Y12"
FT STRAND 122..136
FT /evidence="ECO:0007829|PDB:1Y12"
FT STRAND 150..155
FT /evidence="ECO:0007829|PDB:1Y12"
FT TURN 156..159
FT /evidence="ECO:0007829|PDB:1Y12"
SQ SEQUENCE 162 AA; 17415 MW; BFC45EC5C8107BF5 CRC64;
MAVDMFIKIG DVKGESKDKT HAEEIDVLAW SWGMSQSGSM HMGGGGGAGK VNVQDLSFTK
YIDKSTPNLM MACSSGKHYP QAKLTIRKAG GENQVEYLII TLKEVLVSSV STGGSGGEDR
LTENVTLNFA QVQVDYQPQK ADGAKDGGPV KYGWNIRQNV QA
