ID   HCPA_HELPJ              Reviewed;         250 AA.
AC   Q9ZMM1;
DT   24-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 133.
DE   RecName: Full=Beta-lactamase HcpA;
DE            EC=3.5.2.6;
DE   AltName: Full=Cysteine-rich 28 kDa protein;
DE   Flags: Precursor;
GN   Name=hcpA; OrderedLocusNames=jhp_0197;
OS   Helicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Helicobacteraceae; Helicobacter.
OX   NCBI_TaxID=85963;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=J99 / ATCC 700824;
RX   PubMed=9923682; DOI=10.1038/16495;
RA   Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C.,
RA   Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J.,
RA   Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D.,
RA   Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J.;
RT   "Genomic sequence comparison of two unrelated isolates of the human gastric
RT   pathogen Helicobacter pylori.";
RL   Nature 397:176-180(1999).
CC   -!- FUNCTION: Slowly hydrolyzes 6-aminopenicillinic acid and 7-
CC       aminocephalosporanic acid (ACA) derivatives. May be involved in the
CC       synthesis of the cell wall peptidoglycan (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a beta-lactam + H2O = a substituted beta-amino acid;
CC         Xref=Rhea:RHEA:20401, ChEBI:CHEBI:15377, ChEBI:CHEBI:35627,
CC         ChEBI:CHEBI:140347; EC=3.5.2.6;
CC   -!- ACTIVITY REGULATION: Inhibited by cloxacillin and oxacillin but not by
CC       ACA derivatives or metal chelators. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the hcp beta-lactamase family. {ECO:0000305}.
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DR   EMBL; AE001439; AAD05781.1; -; Genomic_DNA.
DR   PIR; B71961; B71961.
DR   RefSeq; WP_000901652.1; NZ_CP011330.1.
DR   AlphaFoldDB; Q9ZMM1; -.
DR   SMR; Q9ZMM1; -.
DR   STRING; 85963.jhp_0197; -.
DR   EnsemblBacteria; AAD05781; AAD05781; jhp_0197.
DR   KEGG; hpj:jhp_0197; -.
DR   PATRIC; fig|85963.30.peg.823; -.
DR   eggNOG; COG0790; Bacteria.
DR   OMA; NQFACDY; -.
DR   Proteomes; UP000000804; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008800; F:beta-lactamase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 1.25.40.10; -; 1.
DR   InterPro; IPR040239; HcpB-like.
DR   InterPro; IPR006597; Sel1-like.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   InterPro; IPR019734; TPR_repeat.
DR   PANTHER; PTHR13891; PTHR13891; 3.
DR   Pfam; PF08238; Sel1; 6.
DR   SMART; SM00671; SEL1; 6.
DR   SMART; SM00028; TPR; 2.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Disulfide bond; Hydrolase; Repeat; Secreted; Signal;
KW   TPR repeat.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   CHAIN           26..250
FT                   /note="Beta-lactamase HcpA"
FT                   /id="PRO_0000013195"
FT   REPEAT          29..62
FT                   /note="TPR 1"
FT   REPEAT          67..98
FT                   /note="TPR 2"
FT   REPEAT          100..133
FT                   /note="TPR 3"
FT   REPEAT          134..169
FT                   /note="TPR 4"
FT   REPEAT          170..202
FT                   /note="TPR 5"
FT   DISULFID        56..64
FT                   /evidence="ECO:0000250"
FT   DISULFID        92..100
FT                   /evidence="ECO:0000250"
FT   DISULFID        128..136
FT                   /evidence="ECO:0000250"
FT   DISULFID        164..172
FT                   /evidence="ECO:0000250"
FT   DISULFID        196..204
FT                   /evidence="ECO:0000250"
FT   DISULFID        232..240
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   250 AA;  27295 MW;  94EC37877E98C578 CRC64;
     MLGSVKKTLF GVLCLGALCL RGLMAEPDAK ELVSLGIESV KKQDFAQAKA HFEKACELKE
     GFGCVFLGAF YEEGKGVGKD LKKAIQFYTK GCELNDGYGC RLLGNLYYNG QGVSKDAKKA
     SQYYSKSCEL NHAEGCTVLG SLHHYGVGTP KDLRKALDLY EKACDLKDSP GCINAGYMYG
     VAKNFKEAIV RYSKACELKD GRGCYNLGVM QYNAQGTAKD EKQAVENFKK GCKSSVKEAC
     DALKELKIEL