HCPC_HELPY
ID HCPC_HELPY Reviewed; 290 AA.
AC O25728;
DT 15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 129.
DE RecName: Full=Putative beta-lactamase HcpC;
DE EC=3.5.2.6;
DE AltName: Full=Cysteine-rich protein C;
DE Flags: Precursor;
GN Name=hcpC; OrderedLocusNames=HP_1098;
OS Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=85962;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700392 / 26695;
RX PubMed=9252185; DOI=10.1038/41483;
RA Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G.,
RA Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A.,
RA Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N.,
RA Loftus B.J., Richardson D.L., Dodson R.J., Khalak H.G., Glodek A.,
RA McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E.,
RA Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D.,
RA Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S.,
RA Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.;
RT "The complete genome sequence of the gastric pathogen Helicobacter
RT pylori.";
RL Nature 388:539-547(1997).
RN [2]
RP ANTIGENICITY.
RX PubMed=11921447;
RX DOI=10.1002/1615-9861(200203)2:3<313::aid-prot313>3.0.co;2-7;
RA Haas G., Karaali G., Ebermayer K., Metzger W.G., Lamer S., Zimny-Arndt U.,
RA Diescher S., Goebel U.B., Vogt K., Roznowski A.B., Wiedenmann B.J.,
RA Meyer T.F., Aebischer T., Jungblut P.R.;
RT "Immunoproteomics of Helicobacter pylori infection and relation to gastric
RT disease.";
RL Proteomics 2:313-324(2002).
RN [3]
RP ANTIGENICITY.
RX PubMed=12853383; DOI=10.1128/cdli.10.4.542-545.2003;
RA Mittl P.R.E., Luethy L., Reinhardt C., Joller H.;
RT "Detection of high titers of antibody against Helicobacter cysteine-rich
RT proteins A, B, C, and E in Helicobacter pylori-infected individuals.";
RL Clin. Diagn. Lab. Immunol. 10:542-545(2003).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 24-290, AND DISULFIDE BONDS.
RX PubMed=15223324; DOI=10.1016/j.jmb.2004.04.055;
RA Luethy L., Grutter M.G., Mittl P.R.E.;
RT "The crystal structure of Helicobacter cysteine-rich protein C at 2.0 A
RT resolution: similar peptide-binding sites in TPR and SEL1-like repeat
RT proteins.";
RL J. Mol. Biol. 340:829-841(2004).
CC -!- FUNCTION: May hydrolyze 6-aminopenicillinic acid and 7-
CC aminocephalosporanic acid (ACA) derivatives. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a beta-lactam + H2O = a substituted beta-amino acid;
CC Xref=Rhea:RHEA:20401, ChEBI:CHEBI:15377, ChEBI:CHEBI:35627,
CC ChEBI:CHEBI:140347; EC=3.5.2.6;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- MISCELLANEOUS: Antibodies against HcpC are present in sera from human
CC patients infected by Helicobacter pylori.
CC -!- SIMILARITY: Belongs to the hcp beta-lactamase family. {ECO:0000305}.
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DR EMBL; AE000511; AAD08141.1; -; Genomic_DNA.
DR PIR; B64657; B64657.
DR RefSeq; NP_207889.1; NC_000915.1.
DR RefSeq; WP_000892717.1; NC_018939.1.
DR PDB; 1OUV; X-ray; 2.00 A; A=24-290.
DR PDBsum; 1OUV; -.
DR AlphaFoldDB; O25728; -.
DR SMR; O25728; -.
DR DIP; DIP-3502N; -.
DR IntAct; O25728; 1.
DR MINT; O25728; -.
DR STRING; 85962.C694_05665; -.
DR PaxDb; O25728; -.
DR EnsemblBacteria; AAD08141; AAD08141; HP_1098.
DR KEGG; hpy:HP_1098; -.
DR PATRIC; fig|85962.47.peg.1178; -.
DR eggNOG; COG0790; Bacteria.
DR OMA; WYTRLEN; -.
DR PhylomeDB; O25728; -.
DR EvolutionaryTrace; O25728; -.
DR Proteomes; UP000000429; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008800; F:beta-lactamase activity; IEA:UniProtKB-EC.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR040239; HcpB-like.
DR InterPro; IPR006597; Sel1-like.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR PANTHER; PTHR13891; PTHR13891; 2.
DR Pfam; PF08238; Sel1; 7.
DR SMART; SM00671; SEL1; 7.
DR SMART; SM00028; TPR; 3.
DR PROSITE; PS50005; TPR; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antibiotic resistance; Disulfide bond; Hydrolase;
KW Reference proteome; Repeat; Secreted; Signal; TPR repeat.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..290
FT /note="Putative beta-lactamase HcpC"
FT /id="PRO_0000013196"
FT REPEAT 29..62
FT /note="TPR 1"
FT REPEAT 64..98
FT /note="TPR 2"
FT REPEAT 100..133
FT /note="TPR 3"
FT REPEAT 134..170
FT /note="TPR 4"
FT REPEAT 172..205
FT /note="TPR 5"
FT REPEAT 206..242
FT /note="TPR 6"
FT REPEAT 244..278
FT /note="TPR 7"
FT DISULFID 56..64
FT /evidence="ECO:0000269|PubMed:15223324"
FT DISULFID 92..100
FT /evidence="ECO:0000269|PubMed:15223324"
FT DISULFID 128..136
FT /evidence="ECO:0000269|PubMed:15223324"
FT DISULFID 164..172
FT /evidence="ECO:0000269|PubMed:15223324"
FT DISULFID 200..208
FT /evidence="ECO:0000269|PubMed:15223324"
FT DISULFID 236..244
FT /evidence="ECO:0000269|PubMed:15223324"
FT DISULFID 272..280
FT /evidence="ECO:0000269|PubMed:15223324"
FT HELIX 29..41
FT /evidence="ECO:0007829|PDB:1OUV"
FT HELIX 45..57
FT /evidence="ECO:0007829|PDB:1OUV"
FT HELIX 61..73
FT /evidence="ECO:0007829|PDB:1OUV"
FT STRAND 75..77
FT /evidence="ECO:0007829|PDB:1OUV"
FT HELIX 81..93
FT /evidence="ECO:0007829|PDB:1OUV"
FT HELIX 97..109
FT /evidence="ECO:0007829|PDB:1OUV"
FT STRAND 111..113
FT /evidence="ECO:0007829|PDB:1OUV"
FT HELIX 117..129
FT /evidence="ECO:0007829|PDB:1OUV"
FT HELIX 133..145
FT /evidence="ECO:0007829|PDB:1OUV"
FT STRAND 147..149
FT /evidence="ECO:0007829|PDB:1OUV"
FT HELIX 153..165
FT /evidence="ECO:0007829|PDB:1OUV"
FT HELIX 169..181
FT /evidence="ECO:0007829|PDB:1OUV"
FT STRAND 183..185
FT /evidence="ECO:0007829|PDB:1OUV"
FT HELIX 189..201
FT /evidence="ECO:0007829|PDB:1OUV"
FT HELIX 205..217
FT /evidence="ECO:0007829|PDB:1OUV"
FT HELIX 225..237
FT /evidence="ECO:0007829|PDB:1OUV"
FT HELIX 241..252
FT /evidence="ECO:0007829|PDB:1OUV"
FT STRAND 255..258
FT /evidence="ECO:0007829|PDB:1OUV"
FT HELIX 264..274
FT /evidence="ECO:0007829|PDB:1OUV"
FT HELIX 277..284
FT /evidence="ECO:0007829|PDB:1OUV"
SQ SEQUENCE 290 AA; 31594 MW; 0F0CFD779A6F108E CRC64;
MLENVKKSFF RVLCLGALCL GGLMAEQDPK ELVGLGAKSY KEKDFTQAKK YFEKACDLKE
NSGCFNLGVL YYQGQGVEKN LKKAASFYAK ACDLNYSNGC HLLGNLYYSG QGVSQNTNKA
LQYYSKACDL KYAEGCASLG GIYHDGKVVT RDFKKAVEYF TKACDLNDGD GCTILGSLYD
AGRGTPKDLK KALASYDKAC DLKDSPGCFN AGNMYHHGEG ATKNFKEALA RYSKACELEN
GGGCFNLGAM QYNGEGVTRN EKQAIENFKK GCKLGAKGAC DILKQLKIKV
