ID   HCPE_HELPJ              Reviewed;         355 AA.
AC   Q9ZMJ9;
DT   15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 116.
DE   RecName: Full=Putative beta-lactamase HcpE;
DE            EC=3.5.2.6;
DE   AltName: Full=Cysteine-rich protein E;
DE   Flags: Precursor;
GN   Name=hcpE; OrderedLocusNames=jhp_0220;
OS   Helicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Helicobacteraceae; Helicobacter.
OX   NCBI_TaxID=85963;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=J99 / ATCC 700824;
RX   PubMed=9923682; DOI=10.1038/16495;
RA   Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C.,
RA   Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J.,
RA   Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D.,
RA   Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J.;
RT   "Genomic sequence comparison of two unrelated isolates of the human gastric
RT   pathogen Helicobacter pylori.";
RL   Nature 397:176-180(1999).
CC   -!- FUNCTION: May hydrolyze 6-aminopenicillinic acid and 7-
CC       aminocephalosporanic acid (ACA) derivatives. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a beta-lactam + H2O = a substituted beta-amino acid;
CC         Xref=Rhea:RHEA:20401, ChEBI:CHEBI:15377, ChEBI:CHEBI:35627,
CC         ChEBI:CHEBI:140347; EC=3.5.2.6;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the hcp beta-lactamase family. {ECO:0000305}.
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DR   EMBL; AE001439; AAD05807.1; -; Genomic_DNA.
DR   PIR; G71958; G71958.
DR   RefSeq; WP_001022031.1; NZ_CP011330.1.
DR   AlphaFoldDB; Q9ZMJ9; -.
DR   SMR; Q9ZMJ9; -.
DR   STRING; 85963.jhp_0220; -.
DR   EnsemblBacteria; AAD05807; AAD05807; jhp_0220.
DR   KEGG; hpj:jhp_0220; -.
DR   PATRIC; fig|85963.30.peg.794; -.
DR   eggNOG; COG0790; Bacteria.
DR   OMA; IMIYDKG; -.
DR   Proteomes; UP000000804; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008800; F:beta-lactamase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 1.25.40.10; -; 2.
DR   InterPro; IPR040239; HcpB-like.
DR   InterPro; IPR006597; Sel1-like.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   InterPro; IPR019734; TPR_repeat.
DR   PANTHER; PTHR13891; PTHR13891; 3.
DR   Pfam; PF08238; Sel1; 8.
DR   Pfam; PF13181; TPR_8; 1.
DR   SMART; SM00671; SEL1; 8.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Disulfide bond; Hydrolase; Repeat; Secreted; Signal;
KW   TPR repeat.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..355
FT                   /note="Putative beta-lactamase HcpE"
FT                   /id="PRO_0000013201"
FT   REPEAT          27..60
FT                   /note="TPR 1"
FT   REPEAT          63..96
FT                   /note="TPR 2"
FT   REPEAT          98..131
FT                   /note="TPR 3"
FT   REPEAT          132..166
FT                   /note="TPR 4"
FT   REPEAT          202..240
FT                   /note="TPR 5"
FT   REPEAT          245..275
FT                   /note="TPR 6"
FT   REPEAT          276..311
FT                   /note="TPR 7"
FT   REPEAT          312..344
FT                   /note="TPR 8"
FT   DISULFID        54..62
FT                   /evidence="ECO:0000255"
FT   DISULFID        90..98
FT                   /evidence="ECO:0000255"
FT   DISULFID        126..134
FT                   /evidence="ECO:0000255"
FT   DISULFID        160..168
FT                   /evidence="ECO:0000255"
FT   DISULFID        197..205
FT                   /evidence="ECO:0000255"
FT   DISULFID        234..242
FT                   /evidence="ECO:0000255"
FT   DISULFID        270..278
FT                   /evidence="ECO:0000255"
FT   DISULFID        306..314
FT                   /evidence="ECO:0000255"
FT   DISULFID        338..346
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   355 AA;  39320 MW;  30F140204B9BCA3A CRC64;
     MNIKILKILV GGLFFLSLNA HLWGKQDNSF LGIGERAYKS GNYSKAASYF KKACNDGVSE
     GCTQLGIIYE NGQGTRIDYK KALEYYKTAC QADDREGCFG LGGLYDEGLG TAQNYQEAID
     AYAKACVLKH PESCYNLGII YDRKIKGNAA QAVTYYQKSC NFDMAKGCYI LGTAYEKGFL
     EVKQSNHKAV IYYLKACRLN EGQACRALGS LFENGDAGLD EDFEVAFDYL QKACALNNSG
     GCASLGSMYM LGRYVKKDPQ KAFNYFKQAC DMGSAVSCSR MGFMYSQGDT VSKDLRKALD
     NYERGCDMGD EVGCFALAGM YYNMKDKENA IMIYDKGCKL GMKQACENLT KLRGY