ID   HCPE_HELPY              Reviewed;         355 AA.
AC   O25021;
DT   15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 115.
DE   RecName: Full=Putative beta-lactamase HcpE;
DE            EC=3.5.2.6;
DE   AltName: Full=Cysteine-rich protein E;
DE   Flags: Precursor;
GN   Name=hcpE; OrderedLocusNames=HP_0235;
OS   Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Helicobacteraceae; Helicobacter.
OX   NCBI_TaxID=85962;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700392 / 26695;
RX   PubMed=9252185; DOI=10.1038/41483;
RA   Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G.,
RA   Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A.,
RA   Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N.,
RA   Loftus B.J., Richardson D.L., Dodson R.J., Khalak H.G., Glodek A.,
RA   McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E.,
RA   Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D.,
RA   Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S.,
RA   Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.;
RT   "The complete genome sequence of the gastric pathogen Helicobacter
RT   pylori.";
RL   Nature 388:539-547(1997).
RN   [2]
RP   ANTIGENICITY.
RX   PubMed=12853383; DOI=10.1128/cdli.10.4.542-545.2003;
RA   Mittl P.R.E., Luethy L., Reinhardt C., Joller H.;
RT   "Detection of high titers of antibody against Helicobacter cysteine-rich
RT   proteins A, B, C, and E in Helicobacter pylori-infected individuals.";
RL   Clin. Diagn. Lab. Immunol. 10:542-545(2003).
CC   -!- FUNCTION: May hydrolyze 6-aminopenicillinic acid and 7-
CC       aminocephalosporanic acid (ACA) derivatives. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a beta-lactam + H2O = a substituted beta-amino acid;
CC         Xref=Rhea:RHEA:20401, ChEBI:CHEBI:15377, ChEBI:CHEBI:35627,
CC         ChEBI:CHEBI:140347; EC=3.5.2.6;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- MISCELLANEOUS: Antibodies against HcpE are present in sera from human
CC       patients infected by Helicobacter pylori.
CC   -!- SIMILARITY: Belongs to the hcp beta-lactamase family. {ECO:0000305}.
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DR   EMBL; AE000511; AAD07303.1; -; Genomic_DNA.
DR   PIR; C64549; C64549.
DR   RefSeq; NP_207033.1; NC_000915.1.
DR   RefSeq; WP_000538261.1; NC_018939.1.
DR   AlphaFoldDB; O25021; -.
DR   SMR; O25021; -.
DR   STRING; 85962.C694_01185; -.
DR   PaxDb; O25021; -.
DR   EnsemblBacteria; AAD07303; AAD07303; HP_0235.
DR   KEGG; hpy:HP_0235; -.
DR   PATRIC; fig|85962.47.peg.254; -.
DR   eggNOG; COG0790; Bacteria.
DR   OMA; IMIYDKG; -.
DR   PhylomeDB; O25021; -.
DR   Proteomes; UP000000429; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008800; F:beta-lactamase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 1.25.40.10; -; 2.
DR   InterPro; IPR040239; HcpB-like.
DR   InterPro; IPR006597; Sel1-like.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   InterPro; IPR019734; TPR_repeat.
DR   PANTHER; PTHR13891; PTHR13891; 3.
DR   Pfam; PF08238; Sel1; 7.
DR   Pfam; PF13176; TPR_7; 1.
DR   Pfam; PF13181; TPR_8; 1.
DR   SMART; SM00671; SEL1; 9.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Disulfide bond; Hydrolase; Reference proteome;
KW   Repeat; Secreted; Signal; TPR repeat.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..355
FT                   /note="Putative beta-lactamase HcpE"
FT                   /id="PRO_0000013200"
FT   REPEAT          27..60
FT                   /note="TPR 1"
FT   REPEAT          63..96
FT                   /note="TPR 2"
FT   REPEAT          98..131
FT                   /note="TPR 3"
FT   REPEAT          132..166
FT                   /note="TPR 4"
FT   REPEAT          202..240
FT                   /note="TPR 5"
FT   REPEAT          245..275
FT                   /note="TPR 6"
FT   REPEAT          276..311
FT                   /note="TPR 7"
FT   REPEAT          312..344
FT                   /note="TPR 8"
FT   DISULFID        54..62
FT                   /evidence="ECO:0000255"
FT   DISULFID        90..98
FT                   /evidence="ECO:0000255"
FT   DISULFID        126..134
FT                   /evidence="ECO:0000255"
FT   DISULFID        160..168
FT                   /evidence="ECO:0000255"
FT   DISULFID        197..205
FT                   /evidence="ECO:0000255"
FT   DISULFID        234..242
FT                   /evidence="ECO:0000255"
FT   DISULFID        270..278
FT                   /evidence="ECO:0000255"
FT   DISULFID        306..314
FT                   /evidence="ECO:0000255"
FT   DISULFID        338..346
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   355 AA;  39399 MW;  12B6CB9E295BD628 CRC64;
     MGVKFLKILV CGLFFWSLNA HLWGKQDNSF LGVAEKAYKS GNYSKATSYF KKACNDGVSE
     GCTQLGIIYE NGQGTRIDYK KALEYYKTAC QADDREGCFG LGGLYDEGLG TTQNYQEAID
     AYAKACVLKH PESCYNLGII YDRKIKGNAD QAVTYYQKSC NFDMAKGCYV LGVAYEKGFL
     EVKQSNHKAV IYYLKACRLD DGQACRALGS LFENGDAGLD EDFEVAFDYL QKACGLNNSG
     GCASLGSMYM LGRYVKKDPQ KAFNFFKQAC DMGSAVSCSR MGFMYSQGDA VPKDLRKALD
     NYERGCDMGD EVGCFALAGM YYNMKDKENA IMIYDKGCKL GMKQACENLT KLRGY