ID   3SAG_NAJAT              Reviewed;          30 AA.
AC   P0C944;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 1.
DT   25-MAY-2022, entry version 24.
DE   RecName: Full=Cytotoxin najalgesin;
DE   Flags: Fragment;
OS   Naja atra (Chinese cobra).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Elapinae; Naja.
OX   NCBI_TaxID=8656;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SUBUNIT, MASS SPECTROMETRY, TOXIC DOSE, AND
RP   SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=18765245; DOI=10.1016/j.toxicon.2008.06.030;
RA   Jiang W.-J., Liang Y.-X., Han L.-P., Qiu P.-X., Yuan J., Zhao S.-J.;
RT   "Purification and characterization of a novel antinociceptive toxin from
RT   Cobra venom (Naja naja atra).";
RL   Toxicon 52:638-646(2008).
CC   -!- FUNCTION: Analgesic toxin that is blocked by atropine and naloxone,
CC       suggesting the involvement of cholinergic and opiodergic mechanisms in
CC       its antinociceptive effect (PubMed:18765245). Shows cytolytic activity
CC       by forming pore in lipid membranes (By similarity). {ECO:0000250,
CC       ECO:0000269|PubMed:18765245}.
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:18765245}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:18765245}. Target
CC       cell membrane {ECO:0000250|UniProtKB:P60301}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=6741; Method=MALDI; Note=The measured range is
CC       1-?.; Evidence={ECO:0000269|PubMed:18765245};
CC   -!- TOXIC DOSE: LD(50) is 2.61 mg/kg by intraperitoneal injection into
CC       mice. {ECO:0000269|PubMed:18765245}.
CC   -!- MISCELLANEOUS: Is classified as a S-type cytotoxin, since a serine
CC       residue stands at position 28 (Ser-29 in standard classification).
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC       subfamily. Type IA cytotoxin sub-subfamily. {ECO:0000305}.
CC   -!- CAUTION: This fragment is 100% identical to cardiotoxin 5 (AC Q98961)
CC       and cardiotoxin A4b (AC P07525) from the same species. {ECO:0000305}.
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DR   AlphaFoldDB; P0C944; -.
DR   SMR; P0C944; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR003572; Cytotoxin_Cobra.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   PRINTS; PR00282; CYTOTOXIN.
DR   SUPFAM; SSF57302; SSF57302; 1.
PE   1: Evidence at protein level;
KW   Cardiotoxin; Cytolysis; Direct protein sequencing; Disulfide bond;
KW   Membrane; Secreted; Target cell membrane; Target membrane; Toxin.
FT   CHAIN           <1..>30
FT                   /note="Cytotoxin najalgesin"
FT                   /evidence="ECO:0000269|PubMed:18765245"
FT                   /id="PRO_0000371715"
FT   DISULFID        3..21
FT                   /evidence="ECO:0000250|UniProtKB:P60301"
FT   NON_TER         1
FT   NON_TER         30
SQ   SEQUENCE   30 AA;  3483 MW;  887B1A14B46E131B CRC64;
     LKCNKLVPLF YKTCPAGKNL CYKMFMVSNK