ID   ANFA_AZOVI              Reviewed;         533 AA.
AC   P12626;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1989, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Nitrogen fixation protein AnfA;
GN   Name=anfA;
OS   Azotobacter vinelandii.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Azotobacter.
OX   NCBI_TaxID=354;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2722750; DOI=10.1128/jb.171.6.3258-3267.1989;
RA   Joerger R.D., Jacobson M.R., Bishop P.E.;
RT   "Two nifA-like genes required for expression of alternative nitrogenases by
RT   Azotobacter vinelandii.";
RL   J. Bacteriol. 171:3258-3267(1989).
CC   -!- FUNCTION: AnfA is essential for nitrogen fixation under Mo- and V-
CC       deficient conditions. It is required for the regulation of nitrogenase
CC       3 transcription. Interacts with sigma-54.
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DR   EMBL; M26751; AAA22146.1; -; Genomic_DNA.
DR   PIR; A44514; A44514.
DR   AlphaFoldDB; P12626; -.
DR   SMR; P12626; -.
DR   PRIDE; P12626; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 3.30.450.40; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003018; GAF.
DR   InterPro; IPR029016; GAF-like_dom_sf.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR002197; HTH_Fis.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002078; Sigma_54_int.
DR   InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR   InterPro; IPR025943; Sigma_54_int_dom_ATP-bd_2.
DR   InterPro; IPR025944; Sigma_54_int_dom_CS.
DR   Pfam; PF01590; GAF; 1.
DR   Pfam; PF02954; HTH_8; 1.
DR   Pfam; PF00158; Sigma54_activat; 1.
DR   PRINTS; PR01590; HTHFIS.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00065; GAF; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR   PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR   PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR   PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE   4: Predicted;
KW   Activator; ATP-binding; DNA-binding; Nitrogen fixation; Nucleotide-binding;
KW   Transcription; Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..533
FT                   /note="Nitrogen fixation protein AnfA"
FT                   /id="PRO_0000081316"
FT   DOMAIN          46..186
FT                   /note="GAF"
FT   DOMAIN          219..448
FT                   /note="Sigma-54 factor interaction"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   DNA_BIND        501..520
FT                   /note="H-T-H motif"
FT   REGION          33..193
FT                   /note="A domain"
FT   BINDING         247..254
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   BINDING         310..319
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
SQ   SEQUENCE   533 AA;  59547 MW;  0DEFBE02A1CF7695 CRC64;
     MSDQATFEFD TDYFVEEFSH CFTGECRVKM LPILYKISQI ITGNADLADA LSIVLGVMQQ
     HLKMQRGIVT LYDMRAETIF IHDSFGLTEE EKKRGIYAVG EGITGKVVET GKAIVARRLQ
     EHPDFLGRTR VSRNGKAKAA FFCVPIMRAQ KVLGTIAAER VYMNPRLLKQ DVELLTMIAT
     MIAPLVELYL IENIERVRLE NENRRLKHAL KERFKPSNII GNSKPMQEVY ELIHKVASTK
     ATVLILGESG VGKELVANAI HYNSPNAEAA LVTSNCAPLP ENLAESELFG HEKGSFTGAL
     TMHKGCFEQA DGGTIFLDEV GELSPTVQAK LVRVLQNRTF ERVGGSKPVK VDVRIIAATN
     RNLVEMVEQG TFREDLYYRL NVFPITVPPL RERGSDVIAL ADHFVSAFSR ENGKNVKRIS
     TPALNMLMSY HWPGNVRELE NVMERAVILS DDDVIHSYNL PPSLQTSKES GTAFGLTLEE
     KIKAVECEMI VEALKNSSGH IGEAAKELGL ARRMLGVRME RYGISYKSFS RYA