ANFB_MOUSE
ID ANFB_MOUSE Reviewed; 121 AA.
AC P40753;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 2.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Natriuretic peptides B;
DE AltName: Full=Brain natriuretic factor prohormone {ECO:0000250|UniProtKB:P16860};
DE Short=preproBNP {ECO:0000303|PubMed:8182124};
DE Short=proBNP {ECO:0000250|UniProtKB:P16860};
DE AltName: Full=Gamma-brain natriuretic peptide {ECO:0000250|UniProtKB:P07634};
DE AltName: Full=Iso-ANP {ECO:0000250|UniProtKB:P13205};
DE Contains:
DE RecName: Full=Brain natriuretic peptide 45 {ECO:0000303|PubMed:8182124};
DE AltName: Full=5 kDa cardiac natriuretic peptide {ECO:0000250|UniProtKB:P13205};
DE AltName: Full=BNP(77-121) {ECO:0000303|PubMed:8182124};
DE AltName: Full=Brain natriuretic peptide {ECO:0000250|UniProtKB:P13205};
DE Short=BNP {ECO:0000250|UniProtKB:P13205};
DE Flags: Precursor;
GN Name=Nppb;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION (BRAIN NATRIURETIC PEPTIDE 45),
RP SUBCELLULAR LOCATION (BRAIN NATRIURETIC PEPTIDE 45), AND TISSUE SPECIFICITY
RP (BRAIN NATRIURETIC PEPTIDE 45).
RC STRAIN=BALB/cJ; TISSUE=Heart;
RX PubMed=8182124; DOI=10.1172/jci117182;
RA Ogawa Y., Itoh H., Tamura N., Suga S., Yoshimasa T., Uehira M., Matsuda S.,
RA Shiono S., Nishimoto H., Nakao K.;
RT "Molecular cloning of the complementary DNA and gene that encode mouse
RT brain natriuretic peptide and generation of transgenic mice that
RT overexpress the brain natriuretic peptide gene.";
RL J. Clin. Invest. 93:1911-1921(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY (BRAIN
RP NATRIURETIC PEPTIDE 45).
RC STRAIN=BALB/cJ;
RX PubMed=8097440; DOI=10.1161/01.res.72.5.984;
RA Steinhelper M.E.;
RT "Structure, expression, and genomic mapping of the mouse natriuretic
RT peptide type-B gene.";
RL Circ. Res. 72:984-992(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION (BRAIN NATRIURETIC PEPTIDE 45), AND DISRUPTION PHENOTYPE (BRAIN
RP NATRIURETIC PEPTIDE 45).
RX PubMed=10737768; DOI=10.1073/pnas.070371497;
RA Tamura N., Ogawa Y., Chusho H., Nakamura K., Nakao K., Suda M.,
RA Kasahara M., Hashimoto R., Katsuura G., Mukoyama M., Itoh H., Saito Y.,
RA Tanaka I., Otani H., Katsuki M.;
RT "Cardiac fibrosis in mice lacking brain natriuretic peptide.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:4239-4244(2000).
CC -!- FUNCTION: [Brain natriuretic peptide 45]: Cardiac hormone that plays a
CC key role in mediating cardio-renal homeostasis (PubMed:8182124). May
CC also function as a paracrine antifibrotic factor in the heart
CC (PubMed:10737768). Acts by specifically binding and stimulating NPR1 to
CC produce cGMP, which in turn activates effector proteins that drive
CC various biological responses (PubMed:8182124). Likely involved in
CC regulating the extracellular fluid volume and maintaining the fluid-
CC electrolyte balance through natriuresis, diuresis, kaluresis and
CC chloruresis (By similarity). {ECO:0000250|UniProtKB:P13205,
CC ECO:0000269|PubMed:10737768, ECO:0000269|PubMed:8182124}.
CC -!- SUBCELLULAR LOCATION: [Brain natriuretic peptide 45]: Secreted
CC {ECO:0000269|PubMed:8182124}. Note=Detected in blood.
CC {ECO:0000269|PubMed:8182124}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=Long;
CC IsoId=P40753-1; Sequence=Displayed;
CC Name=Short;
CC IsoId=P40753-2; Sequence=VSP_000263;
CC -!- TISSUE SPECIFICITY: [Brain natriuretic peptide 45]: Expressed
CC abundantly in the ventricle, and in a lesser extent in the atrium (at
CC protein level). {ECO:0000269|PubMed:8182124}.
CC -!- PTM: The precursor molecule is proteolytically cleaved by the
CC endoprotease Furin to produce brain natriuretic peptide 45 (By
CC similarity). May undergo further proteolytic cleavage by various
CC proteases such as DPP4, MME and possibly FAP, to give rise to a variety
CC of shorter peptides (By similarity). May be cleaved at Ser-91 by the
CC prolyl endopeptidase FAP (seprase) activity (in vitro) (By similarity).
CC May be degraded by IDE (By similarity). During IDE degradation, the
CC resulting products initially increase the activation of NPR1 and can
CC also stimulate NPR2 to produce cGMP before the fragments are completely
CC degraded and inactivated by IDE (in vitro) (By similarity).
CC {ECO:0000250|UniProtKB:P13205, ECO:0000250|UniProtKB:P16860}.
CC -!- DISRUPTION PHENOTYPE: [Brain natriuretic peptide 45]: Mice are viable
CC and fertile and do not display gross skeletal abnormalities. Fluid-
CC electrolyte balance and blood pressure remain normal although an
CC increased rate of focal fibrotic lesions is observed within the cardiac
CC ventricles. {ECO:0000269|PubMed:10737768}.
CC -!- SIMILARITY: Belongs to the natriuretic peptide family. {ECO:0000305}.
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DR EMBL; D16497; BAA03948.1; -; Genomic_DNA.
DR EMBL; S58667; AAB26344.2; -; Genomic_DNA.
DR EMBL; BC061165; AAH61165.1; -; mRNA.
DR CCDS; CCDS18926.1; -. [P40753-1]
DR PIR; A49144; A49144.
DR PIR; I49548; I49548.
DR RefSeq; NP_001274277.1; NM_001287348.1. [P40753-2]
DR RefSeq; NP_032752.1; NM_008726.5. [P40753-1]
DR AlphaFoldDB; P40753; -.
DR STRING; 10090.ENSMUSP00000099521; -.
DR PhosphoSitePlus; P40753; -.
DR PaxDb; P40753; -.
DR PeptideAtlas; P40753; -.
DR PRIDE; P40753; -.
DR Antibodypedia; 13918; 1550 antibodies from 41 providers.
DR Ensembl; ENSMUST00000103231; ENSMUSP00000099521; ENSMUSG00000029019. [P40753-1]
DR GeneID; 18158; -.
DR KEGG; mmu:18158; -.
DR UCSC; uc008vto.3; mouse. [P40753-1]
DR UCSC; uc008vtp.3; mouse. [P40753-2]
DR CTD; 4879; -.
DR MGI; MGI:97368; Nppb.
DR VEuPathDB; HostDB:ENSMUSG00000029019; -.
DR eggNOG; ENOG502SD0X; Eukaryota.
DR GeneTree; ENSGT00940000154513; -.
DR HOGENOM; CLU_158067_0_0_1; -.
DR InParanoid; P40753; -.
DR OMA; PTGVWKA; -.
DR OrthoDB; 1493586at2759; -.
DR PhylomeDB; P40753; -.
DR TreeFam; TF106304; -.
DR BioGRID-ORCS; 18158; 0 hits in 73 CRISPR screens.
DR ChiTaRS; Nppb; mouse.
DR PRO; PR:P40753; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; P40753; protein.
DR Bgee; ENSMUSG00000029019; Expressed in cardiac muscle of left ventricle and 30 other tissues.
DR ExpressionAtlas; P40753; baseline and differential.
DR Genevisible; P40753; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005615; C:extracellular space; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; ISO:MGI.
DR GO; GO:0032991; C:protein-containing complex; ISO:MGI.
DR GO; GO:0030133; C:transport vesicle; ISO:MGI.
DR GO; GO:0005179; F:hormone activity; ISO:MGI.
DR GO; GO:0051427; F:hormone receptor binding; ISO:MGI.
DR GO; GO:0005102; F:signaling receptor binding; ISO:MGI.
DR GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; ISO:MGI.
DR GO; GO:0014898; P:cardiac muscle hypertrophy in response to stress; IDA:MGI.
DR GO; GO:0006182; P:cGMP biosynthetic process; ISS:UniProtKB.
DR GO; GO:0019934; P:cGMP-mediated signaling; IBA:GO_Central.
DR GO; GO:0031640; P:killing of cells of another organism; ISO:MGI.
DR GO; GO:0030308; P:negative regulation of cell growth; ISO:MGI.
DR GO; GO:1904055; P:negative regulation of cholangiocyte proliferation; ISO:MGI.
DR GO; GO:0048662; P:negative regulation of smooth muscle cell proliferation; ISO:MGI.
DR GO; GO:0003085; P:negative regulation of systemic arterial blood pressure; ISO:MGI.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IBA:GO_Central.
DR GO; GO:0010753; P:positive regulation of cGMP-mediated signaling; ISO:MGI.
DR GO; GO:1903816; P:positive regulation of collecting lymphatic vessel constriction; ISO:MGI.
DR GO; GO:0035815; P:positive regulation of renal sodium excretion; ISO:MGI.
DR GO; GO:0035810; P:positive regulation of urine volume; ISO:MGI.
DR GO; GO:0006457; P:protein folding; ISO:MGI.
DR GO; GO:0007168; P:receptor guanylyl cyclase signaling pathway; ISS:UniProtKB.
DR InterPro; IPR000663; Natr_peptide.
DR InterPro; IPR030480; Natr_peptide_CS.
DR InterPro; IPR002408; Natriuretic_peptide_brain.
DR Pfam; PF00212; ANP; 1.
DR PRINTS; PR00712; BNATPEPTIDE.
DR SMART; SM00183; NAT_PEP; 1.
DR PROSITE; PS00263; NATRIURETIC_PEPTIDE; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Disulfide bond; Hormone; Reference proteome;
KW Secreted; Signal; Vasoactive.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT PEPTIDE 27..121
FT /note="Natriuretic peptides B"
FT /id="PRO_0000001533"
FT PEPTIDE 77..121
FT /note="Brain natriuretic peptide 45"
FT /id="PRO_0000001534"
FT REGION 61..89
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 61..76
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 76..77
FT /note="Cleavage; by FURIN"
FT /evidence="ECO:0000250|UniProtKB:P13205"
FT SITE 91..92
FT /note="Cleavage; by FAP"
FT /evidence="ECO:0000250|UniProtKB:P16860"
FT DISULFID 99..115
FT /evidence="ECO:0000250|UniProtKB:P16860"
FT VAR_SEQ 43
FT /note="Missing (in isoform Short)"
FT /evidence="ECO:0000305"
FT /id="VSP_000263"
FT CONFLICT 27
FT /note="Y -> H (in Ref. 2; AAB26344)"
FT /evidence="ECO:0000305"
FT CONFLICT 71
FT /note="P -> L (in Ref. 2; AAB26344)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 121 AA; 13756 MW; 4F62AC9445E293B9 CRC64;
MDLLKVLSQM ILFLLFLYLS PLGGHSYPLG SPSQSPEQFK MQKLLELIRE KSEEMAQRQL
LKDQGLTKEH PKRVLRSQGS TLRVQQRPQN SKVTHISSCF GHKIDRIGSV SRLGCNALKL
L
