HCS1_SCHPO
ID HCS1_SCHPO Reviewed; 660 AA.
AC O94247;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=DNA polymerase alpha-associated DNA helicase A;
DE EC=3.6.4.12;
GN Name=hcs1; ORFNames=SPCC737.07c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
CC -!- FUNCTION: DNA polymerase alpha-associated DNA helicase which may be
CC involved in DNA replication. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC -!- SUBUNIT: Associates with the hexameric DNA polymerase alpha.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
CC -!- SIMILARITY: Belongs to the DNA2/NAM7 helicase family. {ECO:0000305}.
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DR EMBL; CU329672; CAA20863.1; -; Genomic_DNA.
DR PIR; T41580; T41580.
DR RefSeq; NP_588369.1; NM_001023360.2.
DR AlphaFoldDB; O94247; -.
DR SMR; O94247; -.
DR BioGRID; 275977; 9.
DR STRING; 4896.SPCC737.07c.1; -.
DR MaxQB; O94247; -.
DR PaxDb; O94247; -.
DR EnsemblFungi; SPCC737.07c.1; SPCC737.07c.1:pep; SPCC737.07c.
DR GeneID; 2539412; -.
DR KEGG; spo:SPCC737.07c; -.
DR PomBase; SPCC737.07c; -.
DR VEuPathDB; FungiDB:SPCC737.07c; -.
DR eggNOG; KOG1803; Eukaryota.
DR HOGENOM; CLU_001666_8_2_1; -.
DR InParanoid; O94247; -.
DR OMA; YKRMEST; -.
DR PhylomeDB; O94247; -.
DR PRO; PR:O94247; -.
DR Proteomes; UP000002485; Chromosome III.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0033203; C:DNA helicase A complex; ISO:PomBase.
DR GO; GO:0043596; C:nuclear replication fork; IC:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0043139; F:5'-3' DNA helicase activity; ISO:PomBase.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; ISS:UniProtKB.
DR GO; GO:0036121; F:double-stranded DNA helicase activity; ISS:UniProtKB.
DR GO; GO:0003697; F:single-stranded DNA binding; ISS:UniProtKB.
DR GO; GO:0032508; P:DNA duplex unwinding; ISS:UniProtKB.
DR GO; GO:1903459; P:mitotic DNA replication lagging strand elongation; ISO:PomBase.
DR CDD; cd18808; SF1_C_Upf1; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR041679; DNA2/NAM7-like_C.
DR InterPro; IPR041677; DNA2/NAM7_AAA_11.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF13086; AAA_11; 1.
DR Pfam; PF13087; AAA_12; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cytoplasm; Helicase; Hydrolase; Nucleotide-binding; Nucleus;
KW Reference proteome.
FT CHAIN 1..660
FT /note="DNA polymerase alpha-associated DNA helicase A"
FT /id="PRO_0000353816"
FT BINDING 232..239
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
SQ SEQUENCE 660 AA; 75003 MW; 676128986E5B61CC CRC64;
MSSSNEQICL DEDWIQKFGD REIEFVDEAQ KSEVDETEKS IKRFPLSVLQ RKGLALINLR
IGVVKTGFGG KTIIDFEKDP AFSNGEELPA NSFSPGDVVS IRQDFQSSKK KRPNETDISV
EGVVTRVHER HISVALKSEE DIPSSVTRLS VVKLVNRVTY ERMRHTMLEF KRSIPEYRNS
LFYTLIGRKK ADVSIDQKLI GDIKYFNKEL NASQKKAVKF SIAVKELSLI HGPPGTGKTH
TLVEIIQQLV LRNKRILVCG ASNLAVDNIV DRLSSSGIPM VRLGHPARLL PSILDHSLDV
LSRTGDNGDV IRGISEDIDV CLSKITKTKN GRERREIYKN IRELRKDYRK YEAKTVANIV
SASKVVFCTL HGAGSRQLKG QRFDAVIIDE ASQALEPQCW IPLLGMNKVI LAGDHMQLSP
NVQSKRPYIS MFERLVKSQG DLVKCFLNIQ YRMHELISKF PSDTFYDSKL VPAEEVKKRL
LMDLENVEET ELTDSPIYFY DTLGNYQEDD RSEDMQNFYQ DSKSNHWEAQ IVSYHISGLL
EAGLEAKDIA VVTPYNAQVA LIRQLLKEKG IEVEMGSVDK VQGREKEAII FSLVRSNDVR
EVGFLAEKRR LNVAITRPKR HLCVIGDSNT VKWASEFFHQ WVDFLEENAI VMDIDATMFE
