ID   HCT_NARPS               Reviewed;         436 AA.
AC   A0A2H5AIZ1;
DT   12-AUG-2020, integrated into UniProtKB/Swiss-Prot.
DT   28-FEB-2018, sequence version 1.
DT   03-AUG-2022, entry version 12.
DE   RecName: Full=Hydroxycinnamoyltransferase {ECO:0000303|PubMed:29229969};
DE            EC=2.3.1.- {ECO:0000312|EMBL:AUG71940.1};
GN   Name=HCT {ECO:0000303|PubMed:29229969};
OS   Narcissus pseudonarcissus (Daffodil).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Asparagales; Amaryllidaceae;
OC   Amaryllidoideae; Narcissus.
OX   NCBI_TaxID=39639;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], REVIEW ON THE AMARYLLIDACEAE ALKALOID
RP   METABOLISM, PATHWAY, TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RC   STRAIN=cv. King Alfred; TISSUE=Bulb;
RX   PubMed=29229969; DOI=10.1038/s41598-017-17724-0;
RA   Singh A., Desgagne-Penix I.;
RT   "Transcriptome and metabolome profiling of Narcissus pseudonarcissus 'King
RT   Alfred' reveal components of Amaryllidaceae alkaloid metabolism.";
RL   Sci. Rep. 7:17356-17356(2017).
CC   -!- FUNCTION: Hydroxycinnamoyl transferase that catalyzes the transfer of
CC       an acyl from p-coumaryol-CoA to various acyl acceptors. Can use
CC       feruloyl-CoA and caffeoyl-CoA as acyl donors.
CC       {ECO:0000250|UniProtKB:Q5SMM6}.
CC   -!- PATHWAY: Phenylpropanoid metabolism. {ECO:0000303|PubMed:29229969}.
CC   -!- TISSUE SPECIFICITY: Mostly expressed in stems, and, to a lower extent,
CC       in bulbs. {ECO:0000269|PubMed:29229969}.
CC   -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
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DR   EMBL; MF416095; AUG71940.1; -; mRNA.
DR   AlphaFoldDB; A0A2H5AIZ1; -.
DR   SMR; A0A2H5AIZ1; -.
DR   GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IEA:UniProt.
DR   GO; GO:0009820; P:alkaloid metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.559.10; -; 2.
DR   InterPro; IPR023213; CAT-like_dom_sf.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Alkaloid metabolism; Transferase.
FT   CHAIN           1..436
FT                   /note="Hydroxycinnamoyltransferase"
FT                   /id="PRO_0000450637"
FT   ACT_SITE        154
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q8W1W9"
FT   ACT_SITE        383
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q8W1W9"
SQ   SEQUENCE   436 AA;  47985 MW;  E78D98BD25AF6F2D CRC64;
     MIINIKETTM VRPSQPTPSQ RLWNSNLDLV VPRFHTPSVY FYRRPGNASD FFDARVLKEA
     LGRALVPFYP MAGRLARDED GRVEIDCNGE GVRFVVAETD SAIDEFGDFA PTMELKKLIP
     KVEYGDDISA FPLLVLQITH FKCGGTSLGV GMQHHVADGA SGLHFINSWS DIARGLDIAV
     PPFIDRSLLR ARDPPSPSFP HIEYQPAPSM NTSPAPIQDP TVKSDPTATA VSIFKLTKQQ
     LDLLKSRVSA KYSSYALVAG HVWRCTSIAR GLPDDQRTKL YCATDGRARL QPPLPSGYFG
     NVIFTATPVA DAGEITGEDS GLEAAAGRIQ RALMRMDDEY LRSALDYLEL QPDLSKLVRG
     AHTFRCPNIG LTSWTRLPIH DADFGWGRPI FMGPGGIAYE GLAFMLPSSE GDGSLSIAIS
     LQAEHMIKFQ KLLYEI