ID   ANFC_LITCT              Reviewed;         129 AA.
AC   P20968;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 2.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=C-type natriuretic peptide 1;
DE            Short=CNP I;
DE   Flags: Precursor;
OS   Lithobates catesbeianus (American bullfrog) (Rana catesbeiana).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Lithobates.
OX   NCBI_TaxID=8400;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Brain;
RX   PubMed=8175740; DOI=10.1016/s0021-9258(17)36809-6;
RA   Kojima M., Ohyama Y., Miyamoto K., Minamino N., Kangawa K., Matsuo H.;
RT   "Cloning and characterization of a novel natriuretic peptide in frog (Rana
RT   catesbeiana).";
RL   J. Biol. Chem. 269:13136-13140(1994).
RN   [2]
RP   PROTEIN SEQUENCE OF 108-129.
RC   TISSUE=Brain;
RX   PubMed=2148082; DOI=10.1016/s0006-291x(05)80076-2;
RA   Yoshihara A., Kozawa H., Minamino N., Kangawa K., Matsuo H.;
RT   "Isolation and sequence determination of frog C-type natriuretic peptide.";
RL   Biochem. Biophys. Res. Commun. 173:591-598(1990).
CC   -!- FUNCTION: Hormone which plays a role in endochondral ossification
CC       through regulation of cartilaginous growth plate chondrocytes
CC       proliferation and differentiation. May also be vasoactive and
CC       natriuretic. May be important for freshwater adaptation (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the natriuretic peptide family. {ECO:0000305}.
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DR   EMBL; D17413; BAA04235.1; -; mRNA.
DR   PIR; A36399; A36399.
DR   PIR; A54119; A54119.
DR   AlphaFoldDB; P20968; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   GO; GO:0006182; P:cGMP biosynthetic process; ISS:UniProtKB.
DR   GO; GO:0003418; P:growth plate cartilage chondrocyte differentiation; ISS:UniProtKB.
DR   GO; GO:0003419; P:growth plate cartilage chondrocyte proliferation; ISS:UniProtKB.
DR   GO; GO:0007168; P:receptor guanylyl cyclase signaling pathway; ISS:UniProtKB.
DR   InterPro; IPR002406; C_natriurtcpep.
DR   InterPro; IPR000663; Natr_peptide.
DR   InterPro; IPR030480; Natr_peptide_CS.
DR   Pfam; PF00212; ANP; 1.
DR   PRINTS; PR00713; CNATPEPTIDE.
DR   PRINTS; PR00710; NATPEPTIDES.
DR   SMART; SM00183; NAT_PEP; 1.
DR   PROSITE; PS00263; NATRIURETIC_PEPTIDE; 1.
PE   1: Evidence at protein level;
KW   Cleavage on pair of basic residues; Direct protein sequencing;
KW   Disulfide bond; Hormone; Secreted; Signal; Vasoactive.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   PROPEP          25..107
FT                   /evidence="ECO:0000269|PubMed:2148082"
FT                   /id="PRO_0000001573"
FT   PEPTIDE         108..129
FT                   /note="C-type natriuretic peptide 1"
FT                   /id="PRO_0000001574"
FT   DISULFID        113..129
SQ   SEQUENCE   129 AA;  14656 MW;  47E0E78E32F07307 CRC64;
     MSYKRGTCLG FIMLLMVSHH HTKGKPLSSL QNLSRLLEDN FERSFGSDEA DQQLVPTDSL
     DQLDPELQWN KNRLEQGDSP HVNEMTLQQL LNDPVGTSRR YRQRNKKGYS RGCFGVKLDR
     IGAFSGLGC