HDA_ECO5E
ID HDA_ECO5E Reviewed; 233 AA.
AC B5Z033;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-NOV-2009, sequence version 2.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=DnaA regulatory inactivator Hda {ECO:0000255|HAMAP-Rule:MF_01158};
GN Name=hda {ECO:0000255|HAMAP-Rule:MF_01158};
GN OrderedLocusNames=ECH74115_3718;
OS Escherichia coli O157:H7 (strain EC4115 / EHEC).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=444450;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EC4115 / EHEC;
RX PubMed=22135463; DOI=10.1073/pnas.1107176108;
RA Eppinger M., Mammel M.K., Leclerc J.E., Ravel J., Cebula T.A.;
RT "Genomic anatomy of Escherichia coli O157:H7 outbreaks.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:20142-20147(2011).
CC -!- FUNCTION: Mediates the interaction of DNA replication initiator protein
CC DnaA with DNA polymerase subunit beta sliding clamp (dnaN). Stimulates
CC hydrolysis of ATP-DnaA to ADP-DnaA, rendering DnaA inactive for
CC reinitiation, a process called regulatory inhibition of DnaA or RIDA
CC (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: The active form seems to be an ADP-bound monomer. Forms the
CC RIDA complex (regulatory inactivation of DnaA) of ATP-DnaA, ADP-Hda and
CC the DNA-loaded beta sliding clamp (dnaN). {ECO:0000255|HAMAP-
CC Rule:MF_01158}.
CC -!- SIMILARITY: Belongs to the DnaA family. HdA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01158}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ACI39616.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; CP001164; ACI39616.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_001307333.1; NC_011353.1.
DR AlphaFoldDB; B5Z033; -.
DR SMR; B5Z033; -.
DR GeneID; 66673639; -.
DR KEGG; ecf:ECH74115_3718; -.
DR HOGENOM; CLU_072265_1_1_6; -.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR GO; GO:0032297; P:negative regulation of DNA-templated DNA replication initiation; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01158; Hda; 1.
DR InterPro; IPR020591; Chromosome_initiator_DnaA-like.
DR InterPro; IPR013317; DnaA.
DR InterPro; IPR017788; Hda.
DR InterPro; IPR022864; Hda_Enterobact.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00308; Bac_DnaA; 1.
DR PRINTS; PR00051; DNAA.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR03420; DnaA_homol_Hda; 1.
PE 3: Inferred from homology;
KW DNA replication; DNA replication inhibitor.
FT CHAIN 1..233
FT /note="DnaA regulatory inactivator Hda"
FT /id="PRO_1000137808"
SQ SEQUENCE 233 AA; 26633 MW; D25C7CDF31DAF7DC CRC64;
MNTPAQLSLP LYLPDDETFA SFWPGDNSSL LAALQNVLRQ EHSGYIYLWA REGAGRSHLL
HAACAELSQR GDAVGYVPLD KRTWFVPEVL DGMEHLSLVC IDNIECIAGD ELWEMAIFDL
YNRILESGKT RLLITGDRPP RQLNLGLPDL ASRLDWGQIY KLQPLSDEDK LQALQLRARL
RGFELPEDVG RFLLKRLDRE MRTLFMTLDQ LDRASITAQR KLTIPFVKEI LKL