HDA_ECODH
ID HDA_ECODH Reviewed; 233 AA.
AC B1XAX1;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-NOV-2009, sequence version 2.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=DnaA regulatory inactivator Hda {ECO:0000255|HAMAP-Rule:MF_01158};
GN Name=hda {ECO:0000255|HAMAP-Rule:MF_01158}; OrderedLocusNames=ECDH10B_2662;
OS Escherichia coli (strain K12 / DH10B).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=316385;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / DH10B;
RX PubMed=18245285; DOI=10.1128/jb.01695-07;
RA Durfee T., Nelson R., Baldwin S., Plunkett G. III, Burland V., Mau B.,
RA Petrosino J.F., Qin X., Muzny D.M., Ayele M., Gibbs R.A., Csorgo B.,
RA Posfai G., Weinstock G.M., Blattner F.R.;
RT "The complete genome sequence of Escherichia coli DH10B: insights into the
RT biology of a laboratory workhorse.";
RL J. Bacteriol. 190:2597-2606(2008).
CC -!- FUNCTION: Mediates the interaction of DNA replication initiator protein
CC DnaA with DNA polymerase subunit beta sliding clamp (dnaN). Stimulates
CC hydrolysis of ATP-DnaA to ADP-DnaA, rendering DnaA inactive for
CC reinitiation, a process called regulatory inhibition of DnaA or RIDA
CC (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: The active form seems to be an ADP-bound monomer. Forms the
CC RIDA complex (regulatory inactivation of DnaA) of ATP-DnaA, ADP-Hda and
CC the DNA-loaded beta sliding clamp (dnaN). {ECO:0000255|HAMAP-
CC Rule:MF_01158}.
CC -!- SIMILARITY: Belongs to the DnaA family. HdA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01158}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ACB03648.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; CP000948; ACB03648.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_001307333.1; NC_010473.1.
DR AlphaFoldDB; B1XAX1; -.
DR SMR; B1XAX1; -.
DR PRIDE; B1XAX1; -.
DR GeneID; 66673639; -.
DR KEGG; ecd:ECDH10B_2662; -.
DR HOGENOM; CLU_072265_1_1_6; -.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR GO; GO:0032297; P:negative regulation of DNA-templated DNA replication initiation; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01158; Hda; 1.
DR InterPro; IPR020591; Chromosome_initiator_DnaA-like.
DR InterPro; IPR013317; DnaA.
DR InterPro; IPR017788; Hda.
DR InterPro; IPR022864; Hda_Enterobact.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00308; Bac_DnaA; 1.
DR PRINTS; PR00051; DNAA.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR03420; DnaA_homol_Hda; 1.
PE 3: Inferred from homology;
KW DNA replication; DNA replication inhibitor.
FT CHAIN 1..233
FT /note="DnaA regulatory inactivator Hda"
FT /id="PRO_1000137811"
SQ SEQUENCE 233 AA; 26633 MW; D25C7CDF31DAF7DC CRC64;
MNTPAQLSLP LYLPDDETFA SFWPGDNSSL LAALQNVLRQ EHSGYIYLWA REGAGRSHLL
HAACAELSQR GDAVGYVPLD KRTWFVPEVL DGMEHLSLVC IDNIECIAGD ELWEMAIFDL
YNRILESGKT RLLITGDRPP RQLNLGLPDL ASRLDWGQIY KLQPLSDEDK LQALQLRARL
RGFELPEDVG RFLLKRLDRE MRTLFMTLDQ LDRASITAQR KLTIPFVKEI LKL