ANFD_RHOCA
ID ANFD_RHOCA Reviewed; 526 AA.
AC Q07933;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=Nitrogenase iron-iron protein alpha chain;
DE EC=1.18.6.1;
DE AltName: Full=Dinitrogenase 3 subunit alpha;
DE AltName: Full=Nitrogenase component I;
GN Name=anfD;
OS Rhodobacter capsulatus (Rhodopseudomonas capsulata).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC Rhodobacteraceae; Rhodobacter.
OX NCBI_TaxID=1061;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=B10S;
RX PubMed=8332060; DOI=10.1111/j.1365-2958.1993.tb01611.x;
RA Schueddekopf K., Hennecke S., Liese U., Kutsche M., Klipp W.;
RT "Characterization of anf genes specific for the alternative nitrogenase and
RT identification of nif genes required for both nitrogenases in Rhodobacter
RT capsulatus.";
RL Mol. Microbiol. 8:673-684(1993).
CC -!- FUNCTION: This iron-iron protein is part of the nitrogenase complex
CC that catalyzes the key enzymatic reactions in nitrogen fixation. Other
CC nitrogenase complexes utilize a molybdenum-iron protein or a vanadium-
CC iron protein.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16
CC ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16
CC phosphate; Xref=Rhea:RHEA:21448, Rhea:RHEA-COMP:10000, Rhea:RHEA-
CC COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17997,
CC ChEBI:CHEBI:18276, ChEBI:CHEBI:28938, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:456216; EC=1.18.6.1;
CC -!- COFACTOR:
CC Name=[8Fe-7S] cluster; Xref=ChEBI:CHEBI:21143; Evidence={ECO:0000250};
CC Note=Binds 1 [8Fe-7S] cluster per heterodimer. {ECO:0000250};
CC -!- COFACTOR:
CC Name=[8Fe-9S-C-homocitryl] cluster; Xref=ChEBI:CHEBI:60504;
CC Evidence={ECO:0000250};
CC Note=Binds 1 [8Fe-9S-C-homocitryl] cluster per subunit. {ECO:0000250};
CC -!- SUBUNIT: Hexamer of two alpha, two beta, and two delta chains.
CC -!- MISCELLANEOUS: The structure of the 8Fe-9S-C-homocitryl cluster is
CC assumed to be analogous to the 7Fe-Mo-9S-C-homocitryl cluster.
CC -!- SIMILARITY: Belongs to the NifD/NifK/NifE/NifN family. {ECO:0000305}.
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DR EMBL; X70033; CAA49625.1; -; Genomic_DNA.
DR PIR; S34945; S34945.
DR AlphaFoldDB; Q07933; -.
DR SMR; Q07933; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0018697; F:carbonyl sulfide nitrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016163; F:nitrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR InterPro; IPR000510; Nase/OxRdtase_comp1.
DR InterPro; IPR005974; Nase_asu.
DR InterPro; IPR010143; Nase_comp1_asu.
DR InterPro; IPR000318; Nase_comp1_CS.
DR InterPro; IPR011290; Nase_Fe-Fe_asu.
DR PANTHER; PTHR43457; PTHR43457; 1.
DR Pfam; PF00148; Oxidored_nitro; 1.
DR TIGRFAMs; TIGR01284; alt_nitrog_alph; 1.
DR TIGRFAMs; TIGR01861; ANFD; 1.
DR TIGRFAMs; TIGR01862; N2-ase-Ialpha; 1.
DR PROSITE; PS00699; NITROGENASE_1_1; 1.
DR PROSITE; PS00090; NITROGENASE_1_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Iron; Iron-sulfur; Metal-binding; Nitrogen fixation;
KW Nucleotide-binding; Oxidoreductase.
FT CHAIN 1..526
FT /note="Nitrogenase iron-iron protein alpha chain"
FT /id="PRO_0000153082"
FT REGION 507..526
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 49
FT /ligand="[8Fe-7S] cluster"
FT /ligand_id="ChEBI:CHEBI:21143"
FT /ligand_note="ligand shared with beta chain"
FT /evidence="ECO:0000250"
FT BINDING 75
FT /ligand="[8Fe-7S] cluster"
FT /ligand_id="ChEBI:CHEBI:21143"
FT /ligand_note="ligand shared with beta chain"
FT /evidence="ECO:0000250"
FT BINDING 138
FT /ligand="[8Fe-7S] cluster"
FT /ligand_id="ChEBI:CHEBI:21143"
FT /ligand_note="ligand shared with beta chain"
FT /evidence="ECO:0000250"
FT BINDING 257
FT /ligand="[8Fe-9S-C-homocitryl] cluster"
FT /ligand_id="ChEBI:CHEBI:60504"
FT /evidence="ECO:0000250"
FT BINDING 423
FT /ligand="[8Fe-9S-C-homocitryl] cluster"
FT /ligand_id="ChEBI:CHEBI:60504"
FT /evidence="ECO:0000250"
SQ SEQUENCE 526 AA; 59061 MW; D6AD51EA40421074 CRC64;
MPYHEFEVSK CIPERREHAV MKAAGEDLTS CLPKGYLNTI PGTISERGCA YCGAKHVIGT
PMKDVIHISH GPNGCTYDTW QTKRYISDND NFQLKYTFAT DVKEKHVVFG AEGLLKKSMH
EAFDAFPNIK RMTVYQTCTT ALIGDDVDAI AKEVMEERGD VDVFVCNSPG FAGPSQSGGH
HKINIAWLNQ KVGTVEPDYL GEHVINYVGE YNIQGDQEVM IDYFNRMGIQ VLSTFTGNGS
YDSLRMMHRA HLNVLECARS AEYICDELRA RYGIPRLDID GFGFEPLANS LRKVALFFGI
EDKAEAIIAE EYAKWKPQLD WYKERLKGKK VCLWPGGSKL WHWAHAIEEE MGLKVVSVYT
KFGHQGDMEK GVSRCGEGAL AIDDPNELES VEAIEMLKPD IIFTGKRPGE FVKKHGVPYL
NAHAYHNGPY KGFEGWVRFA RDIYNAIYSP MRQLAALDIS APDAAITSGF RTAKMNADLT
VSDEVKFSEV LHEYTGKYDS IAEIRARNQP MPPSRKLRDA VQPAAE
