HDC_DROME
ID HDC_DROME Reviewed; 1080 AA.
AC Q9N2M8; Q24480; Q95SY2; Q9VA84;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Headcase protein;
DE Contains:
DE RecName: Full=Headcase short protein;
GN Name=hdc; ORFNames=CG15532;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC TISSUE=Embryo;
RX PubMed=8575315; DOI=10.1242/dev.121.12.4149;
RA Weaver T.A., White R.A.;
RT "Headcase, an imaginal specific gene required for adult morphogenesis in
RT Drosophila melanogaster.";
RL Development 121:4149-4160(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP PARTIAL NUCLEOTIDE SEQUENCE, FUNCTION, AND TISSUE SPECIFICITY.
RC TISSUE=Embryo;
RX PubMed=9531534; DOI=10.1101/gad.12.7.956;
RA Steneberg P., Englund C., Kronhamn J., Weaver T.A., Samakovlis C.;
RT "Translational readthrough in the hdc mRNA generates a novel branching
RT inhibitor in the Drosophila trachea.";
RL Genes Dev. 12:956-967(1998).
CC -!- FUNCTION: Required for imaginal cell differentiation, may be involved
CC in hormonal responsiveness during metamorphosis. Involved in an
CC inhibitory signaling mechanism to determine the number of cells that
CC will form unicellular sprouts in the trachea. Regulated by
CC transcription factor esg. The longer hdc protein is completely
CC functional and the shorter protein carries some function.
CC {ECO:0000269|PubMed:8575315, ECO:0000269|PubMed:9531534}.
CC -!- INTERACTION:
CC Q9N2M8; Q86B79: unk; NbExp=3; IntAct=EBI-97141, EBI-90517;
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- TISSUE SPECIFICITY: Expressed in all imaginal cells of the embryo and
CC larvae. Expressed in a subset of tracheal fusion cells from stage 14 to
CC the end of embryogenesis in metameres 2-9, lateral trunk and ventral
CC anastomoses. {ECO:0000269|PubMed:8575315, ECO:0000269|PubMed:9531534}.
CC -!- MISCELLANEOUS: Readthrough of the terminator UAA occurs between codons
CC for Ala-650 and His-652. Readthrough is not always suppressed as the
CC shorter protein is more abundant.
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DR EMBL; Z50097; CAA90425.1; -; mRNA.
DR EMBL; Z50097; CAB58233.1; -; mRNA.
DR EMBL; AE014297; AAF57033.1; -; Genomic_DNA.
DR EMBL; AE014297; AAO41615.1; -; Genomic_DNA.
DR EMBL; AY060431; AAL25470.1; -; mRNA.
DR RefSeq; NP_001263103.1; NM_001276174.1.
DR RefSeq; NP_524592.2; NM_079853.4.
DR RefSeq; NP_788768.1; NM_176591.3.
DR BioGRID; 68460; 10.
DR IntAct; Q9N2M8; 7.
DR STRING; 7227.FBpp0303343; -.
DR PaxDb; Q9N2M8; -.
DR DNASU; 43604; -.
DR EnsemblMetazoa; FBtr0085621; FBpp0084985; FBgn0010113.
DR GeneID; 43604; -.
DR KEGG; dme:Dmel_CG15532; -.
DR UCSC; CG15532-RA; d. melanogaster.
DR FlyBase; FBgn0010113; hdc.
DR VEuPathDB; VectorBase:FBgn0010113; -.
DR eggNOG; KOG3816; Eukaryota.
DR GeneTree; ENSGT00500000044928; -.
DR HOGENOM; CLU_278766_0_0_1; -.
DR InParanoid; Q9N2M8; -.
DR PhylomeDB; Q9N2M8; -.
DR BioGRID-ORCS; 43604; 0 hits in 3 CRISPR screens.
DR ChiTaRS; Hdc; fly.
DR GenomeRNAi; 43604; -.
DR PRO; PR:Q9N2M8; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0010113; Expressed in eye disc (Drosophila) and 30 other tissues.
DR ExpressionAtlas; Q9N2M8; baseline and differential.
DR Genevisible; Q9N2M8; DM.
DR GO; GO:0030424; C:axon; IDA:FlyBase.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0030425; C:dendrite; IDA:FlyBase.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:FlyBase.
DR GO; GO:0002165; P:instar larval or pupal development; IMP:UniProtKB.
DR GO; GO:0050768; P:negative regulation of neurogenesis; IMP:FlyBase.
DR GO; GO:0035194; P:post-transcriptional gene silencing by RNA; IMP:FlyBase.
DR GO; GO:1904799; P:regulation of neuron remodeling; IMP:FlyBase.
DR GO; GO:0035071; P:salivary gland cell autophagic cell death; IMP:FlyBase.
DR GO; GO:0007430; P:terminal branching, open tracheal system; IMP:UniProtKB.
DR InterPro; IPR026066; Headcase.
DR InterPro; IPR031947; Headcase_mid.
DR PANTHER; PTHR13425; PTHR13425; 1.
DR Pfam; PF16002; Headcase; 1.
DR Pfam; PF15353; HECA; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Developmental protein; Reference proteome.
FT CHAIN 1..1080
FT /note="Headcase protein"
FT /id="PRO_0000021402"
FT CHAIN 1..650
FT /note="Headcase short protein"
FT /id="PRO_0000021403"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 179..281
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 309..341
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 650..735
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 764..852
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 891..997
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 179..195
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 230..249
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 257..281
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 311..341
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 660..692
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 721..735
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 764..789
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 796..852
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 891..931
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 940..977
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 85
FT /note="H -> P (in Ref. 1; CAA90425/CAB58233)"
FT /evidence="ECO:0000305"
FT CONFLICT 190..191
FT /note="PT -> SN (in Ref. 1; CAA90425/CAB58233)"
FT /evidence="ECO:0000305"
FT CONFLICT 226
FT /note="A -> G (in Ref. 1; CAA90425/CAB58233)"
FT /evidence="ECO:0000305"
FT CONFLICT 243..244
FT /note="SY -> HD (in Ref. 1; CAA90425/CAB58233)"
FT /evidence="ECO:0000305"
FT CONFLICT 279..310
FT /note="SGVLQTSALATFSNILNTNNVLGLDLRARAGS -> PACCRPVRWPLSATSS
FT IRTMSWPGPARQGWQ (in Ref. 1; CAA90425)"
FT /evidence="ECO:0000305"
FT CONFLICT 342
FT /note="P -> A (in Ref. 1; CAA90425/CAB58233)"
FT /evidence="ECO:0000305"
FT CONFLICT 353
FT /note="L -> V (in Ref. 1; CAA90425/CAB58233)"
FT /evidence="ECO:0000305"
FT CONFLICT 383
FT /note="Q -> P (in Ref. 1; CAA90425/CAB58233)"
FT /evidence="ECO:0000305"
FT CONFLICT 432
FT /note="D -> E (in Ref. 1; CAA90425/CAB58233)"
FT /evidence="ECO:0000305"
FT CONFLICT 641
FT /note="T -> S (in Ref. 1; CAA90425/CAB58233)"
FT /evidence="ECO:0000305"
FT CONFLICT 695
FT /note="P -> Q (in Ref. 1; CAB58233)"
FT /evidence="ECO:0000305"
FT CONFLICT 852
FT /note="S -> SS (in Ref. 1; CAB58233)"
FT /evidence="ECO:0000305"
FT CONFLICT 1067
FT /note="A -> R (in Ref. 1; CAB58233)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1080 AA; 117448 MW; 87EB144BA0D1B787 CRC64;
MAPRRNSNIS SSGNSTQQQH QQQLQQQHQT QQHQLLQFYA ENVNSSGVLM APMQGTGGVG
GVGGGGMVHC CLPSGDCRKL DTLIHLNELS LADCIRVLCN NENCSAGQYM HRECFEWWEA
SVLQALKANG RARSWSERQR LQHLWTKKGY ELVQKACSCK CGRGQLRKDL DWVPPSSQGV
IYLNGSGNRP TLANGSLSED DDKKKAKKKR NRNNNNNNGG GGGGGAGVNG NTKTPLSNNN
GNSYAGLTPN PNVGVIGSGL PHNNGNTASN GSSGNNGSSG VLQTSALATF SNILNTNNVL
GLDLRARAGS LSSSGAGSGS TSPSDSQSSG EISVSPVQQL LPQQQQQQQQ SLLIQPLGPA
FGSNLLQNGL GLSKNLLIAP QQQQQQQLQQ QQQQQNNLPA LANISNFKPL ASYEQQQLVQ
QQKNKEVELY SDRVRSTSGC NGIFSRRLDF SSFNLLPKTR LNSYQVKIED EGNHGNDETR
LFILSSLAQS QMSRVACILC EEPLLVFDRY PLVDGSFFLS PKQHSSGCIE VKYEGRTLYL
TCVCMSCLDG TSSSRAINCR FCKEPWDGSS LVLGTMYAYD IFAAMPCCAE RFKCNNCFKM
LMHPQQRLSF YSDYSHGVTC PYCNTQDTHF VKPLTFCYAK TTATRLPTLA XHEAPLESPV
GTGATTTQVP NAQGSPTASG CSSNTIASKQ PPKQPLYNAA IDYSAPLQQQ INPDLIGAHP
HAQQHQQQVR QQQQQQQQQQ PQQQQQQQQQ QTQQQQSQQQ QQQQQQQHQP VVSTFQRGNF
PQQSHKTMNM MAMADVMSKY QQQQHQQQQQ QRQQQHNLQP QQQHATQKGL EGLLLTNTNT
SNNNSSSSSS SSISNLIISH NSASNTGNKM QPTWGQSDAI SALWGCSSSS SGCSSGSGSQ
PSLSPTASSN GNDGSKMMLW AAQSSPQNAA TLRDGFKELQ RQQPPQQQVP QQQPHAASPT
ASLTSSSSSS NGWSASHYGK PNIWELPGST GQRTPASSHD IFTDLLCNLS ISQDNSSQQA
SSKADASDVS SNSSSSAGEL LEAANIWRFP EYASSQLYME EPTGGAAACM QLFNDYLNMN
