ID   HDDL_ALCXX              Reviewed;         296 AA.
AC   Q59168;
DT   01-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 62.
DE   RecName: Full=2-haloacid dehalogenase, configuration-inverting;
DE            EC=3.8.1.10;
DE   AltName: Full=DL-2-haloacid dehalogenase;
DE   AltName: Full=DL-DEXi;
GN   Name=dhlC; Synonyms=dhlIV;
OS   Alcaligenes xylosoxydans xylosoxydans (Achromobacter xylosoxidans).
OG   Plasmid pFL40.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Alcaligenaceae; Achromobacter.
OX   NCBI_TaxID=85698;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9144969; DOI=10.1007/bf00056422;
RA   Brokamp A., Happe B., Schmidt F.R.J.;
RT   "Cloning and nucleotide sequence of a D,L-haloalkanoic acid dehalogenase
RT   encoding gene from Alcaligenes xylosoxidans ssp. denitrificans ABIV.";
RL   Biodegradation 7:383-396(1996).
CC   -!- FUNCTION: Dehalogenates both (S)- and (R)-2-haloalkanoic acids to the
CC       corresponding (R)- and (S)-hydroxyalkanoic acids, respectively, with
CC       inversion of configuration at C-2. Acts on 2-haloalkanoic acids whose
CC       carbon chain lengths are five or less.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an (S)-2-haloacid + H2O = a (2R)-2-hydroxycarboxylate + a
CC         halide anion + H(+); Xref=Rhea:RHEA:11192, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16042, ChEBI:CHEBI:58314,
CC         ChEBI:CHEBI:137405; EC=3.8.1.10;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an (R)-2-haloacid + H2O = a (2S)-2-hydroxycarboxylate + a
CC         halide anion + H(+); Xref=Rhea:RHEA:22188, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16042, ChEBI:CHEBI:58123,
CC         ChEBI:CHEBI:137406; EC=3.8.1.10;
CC   -!- SIMILARITY: Belongs to the HAD-like hydrolase superfamily. S-2-
CC       haloalkanoic acid dehalogenase family. {ECO:0000305}.
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DR   EMBL; X77610; CAA54701.1; -; Genomic_DNA.
DR   PIR; S42024; S42024.
DR   AlphaFoldDB; Q59168; -.
DR   SMR; Q59168; -.
DR   BRENDA; 3.8.1.10; 238.
DR   GO; GO:0018784; F:(S)-2-haloacid dehalogenase activity; IEA:RHEA.
DR   GO; GO:0018778; F:DL-2 haloacid dehalogenase activity; IEA:UniProtKB-EC.
DR   InterPro; IPR019714; 2-haloacid_dehalogenase_DehI.
DR   Pfam; PF10778; DehI; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Plasmid.
FT   CHAIN           1..296
FT                   /note="2-haloacid dehalogenase, configuration-inverting"
FT                   /id="PRO_0000079171"
SQ   SEQUENCE   296 AA;  32785 MW;  0240EB5CE3D5EFA7 CRC64;
     MTNPAYFPQL SQLDVSGEME STYEDIRLTL RVPWVAFGCR VLATFPGYLP LAWRRSAEAL
     ITRYAEQAAD ELRERSLLNI GPLPNLKERL YAAGFDDGEI EKVRRVLYAF NYGNPKYLLL
     ITALSESMQM RPVGGAEVSS ELRASIPKGH PKGMDPLLPL VDATKASTEV QGLLKRVADL
     HYHHGPASDF QALANWPKVL QIVTDEVLAP VARTEQYDAK SRELVTRARE LVRGLPGSAG
     VQRSELMSML TPNELAGLTG VLFMYQRFIA DITISIIHIT ECLDGAEAAS KSPFPI