ID   ANFG_CLOPA              Reviewed;         116 AA.
AC   Q46244;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Nitrogenase iron-iron protein delta chain;
DE            EC=1.18.6.1;
DE   AltName: Full=Dinitrogenase 3 subunit delta;
DE   AltName: Full=Nitrogenase component I;
GN   Name=anfG;
OS   Clostridium pasteurianum.
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=1501;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 6013 / DSM 525 / NCIB 9486 / VKM B-1774 / W5;
RX   PubMed=8334167; DOI=10.1016/0167-4781(93)90096-v;
RA   Zinoni F., Robson R.M., Robson R.L.;
RT   "Organization of potential alternative nitrogenase genes from Clostridium
RT   pasteurianum.";
RL   Biochim. Biophys. Acta 1174:83-86(1993).
CC   -!- FUNCTION: The key enzymatic reactions in nitrogen fixation are
CC       catalyzed by the nitrogenase complex, which has 2 components: the iron
CC       protein (component 2) and a component 1 which is either a molybdenum-
CC       iron protein, a vanadium-iron, or an iron-iron protein.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16
CC         ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16
CC         phosphate; Xref=Rhea:RHEA:21448, Rhea:RHEA-COMP:10000, Rhea:RHEA-
CC         COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17997,
CC         ChEBI:CHEBI:18276, ChEBI:CHEBI:28938, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:456216; EC=1.18.6.1;
CC   -!- COFACTOR:
CC       Name=iron-sulfur cluster; Xref=ChEBI:CHEBI:30408;
CC         Evidence={ECO:0000250};
CC   -!- SUBUNIT: Hexamer of two alpha, two beta, and two delta chains.
CC       {ECO:0000250}.
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DR   EMBL; L09762; AAC36971.1; -; Genomic_DNA.
DR   PIR; S34611; S34611.
DR   RefSeq; WP_003447705.1; NZ_LFYL01000002.1.
DR   AlphaFoldDB; Q46244; -.
DR   SMR; Q46244; -.
DR   PRIDE; Q46244; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0018697; F:carbonyl sulfide nitrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0016163; F:nitrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   InterPro; IPR014278; Nase_Fe-Fe_dsu.
DR   InterPro; IPR004349; V/Nase_d_su.
DR   Pfam; PF03139; AnfG_VnfG; 1.
DR   TIGRFAMs; TIGR02929; anfG_nitrog; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Iron; Iron-sulfur; Metal-binding; Nitrogen fixation;
KW   Nucleotide-binding; Oxidoreductase.
FT   CHAIN           1..116
FT                   /note="Nitrogenase iron-iron protein delta chain"
FT                   /id="PRO_0000213566"
SQ   SEQUENCE   116 AA;  13714 MW;  743A7C925B625780 CRC64;
     MEDPSKVQLN QLTDYIMKNC LWQFHSRKWD RERQNEGILT KTKQILLGEE VDLSTPADRC
     YYADALCLAD AYKTEYPWIN DMSKDELIQL MQQLKDRIDY VTITGSLNAE LTDPRY