HDFR_ECOLI
ID HDFR_ECOLI Reviewed; 279 AA.
AC P0A8R9; P22788; P27826; Q2M874; Q9KHQ8;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2005, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=HTH-type transcriptional regulator HdfR;
DE AltName: Full=H-NS-dependent flhDC regulator;
GN Name=hdfR; Synonyms=pssR, yifA, yifD; OrderedLocusNames=b4480, JW5607;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=1995430; DOI=10.1016/0378-1119(91)90005-v;
RA Coppola G., Huang F., Riley J., Cox J.L., Hantzopoulos P., Zhou L.-B.,
RA Calhoun D.H.;
RT "Sequence and transcriptional activity of the Escherichia coli K-12
RT chromosome region between rrnC and ilvGMEDA.";
RL Gene 97:21-27(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=K12;
RX PubMed=10913108; DOI=10.1128/jb.182.16.4670-4672.2000;
RA Ko M., Park C.;
RT "H-NS-dependent regulation of flagellar synthesis is mediated by a LysR
RT family protein.";
RL J. Bacteriol. 182:4670-4672(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=1379743; DOI=10.1126/science.1379743;
RA Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.;
RT "Analysis of the Escherichia coli genome: DNA sequence of the region from
RT 84.5 to 86.5 minutes.";
RL Science 257:771-778(1992).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP SEQUENCE REVISION.
RX PubMed=16397293; DOI=10.1093/nar/gkj405;
RA Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R.,
RA Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T.,
RA Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H.,
RA Thomson N.R., Wishart D., Wanner B.L.;
RT "Escherichia coli K-12: a cooperatively developed annotation snapshot
RT -- 2005.";
RL Nucleic Acids Res. 34:1-9(2006).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [7]
RP PRELIMINARY FUNCTION.
RX PubMed=6309791; DOI=10.1016/s0021-9258(17)44591-1;
RA Sparrow C.P., Raetz C.R.H.;
RT "A trans-acting regulatory mutation that causes overproduction of
RT phosphatidylserine synthase in Escherichia coli.";
RL J. Biol. Chem. 258:9963-9967(1983).
CC -!- FUNCTION: Negatively regulates the transcription of the flagellar
CC master operon flhDC by binding to the upstream region of the operon.
CC {ECO:0000269|PubMed:10913108}.
CC -!- MISCELLANEOUS: The expression of the hdfR gene is negatively regulated
CC by H-NS.
CC -!- SIMILARITY: Belongs to the LysR transcriptional regulatory family.
CC {ECO:0000305}.
CC -!- CAUTION: Was originally thought to be involved in the expression of the
CC phosphatidylserine synthetase pssA. {ECO:0000305|PubMed:6309791}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA24611.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAA67566.1; Type=Frameshift; Note=Produces two separate ORFs.; Evidence={ECO:0000305};
CC Sequence=AAA67567.1; Type=Frameshift; Note=Produces two separate ORFs.; Evidence={ECO:0000305};
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DR EMBL; M37337; AAA24611.1; ALT_FRAME; Genomic_DNA.
DR EMBL; AF251030; AAF97915.1; -; Genomic_DNA.
DR EMBL; M87049; AAA67567.1; ALT_FRAME; Genomic_DNA.
DR EMBL; M87049; AAA67566.1; ALT_FRAME; Genomic_DNA.
DR EMBL; U00096; AAT48206.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77532.1; -; Genomic_DNA.
DR PIR; E65179; E65179.
DR RefSeq; WP_000379245.1; NZ_SSZK01000025.1.
DR RefSeq; YP_026246.1; NC_000913.3.
DR AlphaFoldDB; P0A8R9; -.
DR SMR; P0A8R9; -.
DR BioGRID; 4261482; 6.
DR BioGRID; 853448; 5.
DR DIP; DIP-48185N; -.
DR IntAct; P0A8R9; 5.
DR STRING; 511145.b4480; -.
DR jPOST; P0A8R9; -.
DR PaxDb; P0A8R9; -.
DR PRIDE; P0A8R9; -.
DR EnsemblBacteria; AAT48206; AAT48206; b4480.
DR EnsemblBacteria; BAE77532; BAE77532; BAE77532.
DR GeneID; 2847698; -.
DR GeneID; 58459581; -.
DR KEGG; ecj:JW5607; -.
DR KEGG; eco:b4480; -.
DR PATRIC; fig|1411691.4.peg.2944; -.
DR EchoBASE; EB1418; -.
DR eggNOG; COG0583; Bacteria.
DR HOGENOM; CLU_039613_8_2_6; -.
DR InParanoid; P0A8R9; -.
DR OMA; ESLMNTW; -.
DR PhylomeDB; P0A8R9; -.
DR BioCyc; EcoCyc:EG11449-MON; -.
DR PRO; PR:P0A8R9; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:EcoCyc.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:EcoCyc.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR HAMAP; MF_01233; HTH_type_HdfR; 1.
DR InterPro; IPR005119; LysR_subst-bd.
DR InterPro; IPR020890; Tscrpt_reg_HTH_HdfR.
DR InterPro; IPR000847; Tscrpt_reg_HTH_LysR.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00126; HTH_1; 1.
DR Pfam; PF03466; LysR_substrate; 1.
DR PRINTS; PR00039; HTHLYSR.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS50931; HTH_LYSR; 1.
PE 3: Inferred from homology;
KW DNA-binding; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..279
FT /note="HTH-type transcriptional regulator HdfR"
FT /id="PRO_0000105632"
FT DOMAIN 1..58
FT /note="HTH lysR-type"
FT DNA_BIND 18..37
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
SQ SEQUENCE 279 AA; 31746 MW; 450FF5A542BDE903 CRC64;
MDTELLKTFL EVSRTRHFGR AAESLYLTQS AVSFRIRQLE NQLGVNLFTR HRNNIRLTAA
GEKLLPYAET LMSTWQAARK EVAHTSRHNE FSIGASASLW ECMLNQWLGR LYQNQDAHTG
LQFEARIAQR QSLVKQLHER QLDLLITTEA PKMDEFSSQL LGYFTLALYT SAPSKLKGDL
NYLRLEWGPD FQQHEAGLIG ADEVPILTTS SAELAQQQIA MLNGCTWLPV SWARKKGGLH
TVVDSTTLSR PLYAIWLQNS DKNALIRDLL KINVLDEVY
