ID   HDG_METAC               Reviewed;         158 AA.
AC   Q8TTH3;
DT   15-MAR-2017, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=Hypoxanthine DNA glycosylase {ECO:0000303|PubMed:21642431};
DE            Short=HDG {ECO:0000303|PubMed:21642431};
DE            EC=3.2.2.- {ECO:0000269|PubMed:21642431};
GN   OrderedLocusNames=MA_0462 {ECO:0000312|EMBL:AAM03908.1};
OS   Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 /
OS   C2A).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=188937;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A;
RX   PubMed=11932238; DOI=10.1101/gr.223902;
RA   Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W.,
RA   Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J.,
RA   Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.,
RA   McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D.,
RA   Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R.,
RA   Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J.,
RA   Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A.,
RA   White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H.,
RA   Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V.,
RA   Zinder S.H., Lander E., Metcalf W.W., Birren B.;
RT   "The genome of Methanosarcina acetivorans reveals extensive metabolic and
RT   physiological diversity.";
RL   Genome Res. 12:532-542(2002).
RN   [2]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=21642431; DOI=10.1074/jbc.m111.249524;
RA   Lee H.W., Dominy B.N., Cao W.;
RT   "New family of deamination repair enzymes in uracil-DNA glycosylase
RT   superfamily.";
RL   J. Biol. Chem. 286:31282-31287(2011).
RN   [3] {ECO:0007744|PDB:2L3F}
RP   STRUCTURE BY NMR.
RA   Aramini J.M., Hamilton K., Ciccosanti C.T., Wang H., Lee H.W., Rost B.,
RA   Acton T.B., Xiao R., Everett J.K., Montelione G.T.;
RT   "Solution NMR structure of a putative uracil DNA glycosylase from
RT   Methanosarcina acetivorans, Northeast structural genomics consortium target
RT   mvr76.";
RL   Submitted (SEP-2010) to the PDB data bank.
CC   -!- FUNCTION: Excises hypoxanthine, a deamination product of adenine, from
CC       double-stranded DNA. Acts on double-stranded DNA containing G/I, T/I,
CC       A/I and C/I base pairs, but not on single-stranded inosine-containing
CC       DNA. Has also minor xanthine DNA glycosylase activity. Lacks any
CC       detectable uracil-DNA glycosylase activity.
CC       {ECO:0000269|PubMed:21642431}.
CC   -!- SIMILARITY: Belongs to the uracil-DNA glycosylase (UDG) superfamily.
CC       Type 6 (HDG) family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE010299; AAM03908.1; -; Genomic_DNA.
DR   RefSeq; WP_011020513.1; NC_003552.1.
DR   PDB; 2L3F; NMR; -; A=1-158.
DR   PDBsum; 2L3F; -.
DR   AlphaFoldDB; Q8TTH3; -.
DR   SMR; Q8TTH3; -.
DR   STRING; 188937.MA_0462; -.
DR   DNASU; 1472354; -.
DR   EnsemblBacteria; AAM03908; AAM03908; MA_0462.
DR   GeneID; 1472354; -.
DR   KEGG; mac:MA_0462; -.
DR   HOGENOM; CLU_094865_0_1_2; -.
DR   InParanoid; Q8TTH3; -.
DR   OMA; QQYYAHP; -.
DR   OrthoDB; 103030at2157; -.
DR   PhylomeDB; Q8TTH3; -.
DR   EvolutionaryTrace; Q8TTH3; -.
DR   Proteomes; UP000002487; Chromosome.
DR   GO; GO:0097507; F:hypoxanthine DNA N-glycosylase activity; IDA:UniProtKB.
DR   GO; GO:0006281; P:DNA repair; IDA:UniProtKB.
DR   Gene3D; 3.40.470.10; -; 1.
DR   InterPro; IPR026353; Hypoxan-DNA_Glyclase.
DR   InterPro; IPR005122; Uracil-DNA_glycosylase-like.
DR   InterPro; IPR036895; Uracil-DNA_glycosylase-like_sf.
DR   Pfam; PF03167; UDG; 1.
DR   SMART; SM00986; UDG; 1.
DR   SUPFAM; SSF52141; SSF52141; 1.
DR   TIGRFAMs; TIGR04274; hypoxanDNAglyco; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA damage; DNA repair; Hydrolase; Reference proteome.
FT   CHAIN           1..158
FT                   /note="Hypoxanthine DNA glycosylase"
FT                   /id="PRO_0000439189"
FT   ACT_SITE        39
FT                   /evidence="ECO:0000250|UniProtKB:Q46EH7"
FT   STRAND          15..22
FT                   /evidence="ECO:0007829|PDB:2L3F"
FT   HELIX           25..30
FT                   /evidence="ECO:0007829|PDB:2L3F"
FT   HELIX           33..35
FT                   /evidence="ECO:0007829|PDB:2L3F"
FT   HELIX           41..49
FT                   /evidence="ECO:0007829|PDB:2L3F"
FT   HELIX           58..67
FT                   /evidence="ECO:0007829|PDB:2L3F"
FT   STRAND          70..79
FT                   /evidence="ECO:0007829|PDB:2L3F"
FT   STRAND          83..86
FT                   /evidence="ECO:0007829|PDB:2L3F"
FT   HELIX           100..103
FT                   /evidence="ECO:0007829|PDB:2L3F"
FT   STRAND          109..114
FT                   /evidence="ECO:0007829|PDB:2L3F"
FT   HELIX           115..118
FT                   /evidence="ECO:0007829|PDB:2L3F"
FT   HELIX           121..124
FT                   /evidence="ECO:0007829|PDB:2L3F"
FT   HELIX           125..127
FT                   /evidence="ECO:0007829|PDB:2L3F"
FT   STRAND          130..134
FT                   /evidence="ECO:0007829|PDB:2L3F"
FT   HELIX           139..142
FT                   /evidence="ECO:0007829|PDB:2L3F"
FT   HELIX           149..156
FT                   /evidence="ECO:0007829|PDB:2L3F"
SQ   SEQUENCE   158 AA;  18051 MW;  7E8F20ACCAF0ECAA CRC64;
     MIKRGFPAVL DENTEILILG SLPSDESIRK QQYYGNPGND FWRLVGHAIG ENLQDMAYEK
     KLKTLKHNRI GLWDVFKAGS REGSQDSKIG DEEINDFSGL KEMVPKLRLI CFNGRKAGEY
     EPLLRGMGYE TKVLPSSSGA NRRFSKNRES EWEAVFRH