ID   HDG_METBF               Reviewed;         158 AA.
AC   Q46EH7;
DT   15-MAR-2017, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=Hypoxanthine DNA glycosylase {ECO:0000303|PubMed:21642431};
DE            Short=HDG {ECO:0000303|PubMed:21642431};
DE            EC=3.2.2.- {ECO:0000269|PubMed:21642431};
GN   OrderedLocusNames=Mbar_A0737 {ECO:0000312|EMBL:AAZ69715.1};
OS   Methanosarcina barkeri (strain Fusaro / DSM 804).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=269797;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Fusaro / DSM 804;
RX   PubMed=16980466; DOI=10.1128/jb.00810-06;
RA   Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S.,
RA   Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.;
RT   "The Methanosarcina barkeri genome: comparative analysis with
RT   Methanosarcina acetivorans and Methanosarcina mazei reveals extensive
RT   rearrangement within methanosarcinal genomes.";
RL   J. Bacteriol. 188:7922-7931(2006).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, ACTIVE SITE, AND MUTAGENESIS OF ASN-39;
RP   ASP-74; ASP-86 AND ASN-113.
RX   PubMed=21642431; DOI=10.1074/jbc.m111.249524;
RA   Lee H.W., Dominy B.N., Cao W.;
RT   "New family of deamination repair enzymes in uracil-DNA glycosylase
RT   superfamily.";
RL   J. Biol. Chem. 286:31282-31287(2011).
CC   -!- FUNCTION: Excises hypoxanthine, a deamination product of adenine, from
CC       double-stranded DNA. Acts on double-stranded DNA containing G/I, T/I,
CC       A/I and C/I base pairs, but not on single-stranded inosine-containing
CC       DNA. Has also minor xanthine DNA glycosylase activity. Lacks any
CC       detectable uracil-DNA glycosylase activity.
CC       {ECO:0000269|PubMed:21642431}.
CC   -!- SIMILARITY: Belongs to the uracil-DNA glycosylase (UDG) superfamily.
CC       Type 6 (HDG) family. {ECO:0000305}.
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DR   EMBL; CP000099; AAZ69715.1; -; Genomic_DNA.
DR   RefSeq; WP_011305765.1; NC_007355.1.
DR   AlphaFoldDB; Q46EH7; -.
DR   SMR; Q46EH7; -.
DR   STRING; 269797.Mbar_A0737; -.
DR   EnsemblBacteria; AAZ69715; AAZ69715; Mbar_A0737.
DR   GeneID; 3624438; -.
DR   KEGG; mba:Mbar_A0737; -.
DR   eggNOG; arCOG04947; Archaea.
DR   HOGENOM; CLU_094865_0_1_2; -.
DR   OMA; QQYYAHP; -.
DR   OrthoDB; 103030at2157; -.
DR   GO; GO:0097507; F:hypoxanthine DNA N-glycosylase activity; IDA:UniProtKB.
DR   GO; GO:0006281; P:DNA repair; IDA:UniProtKB.
DR   Gene3D; 3.40.470.10; -; 1.
DR   InterPro; IPR026353; Hypoxan-DNA_Glyclase.
DR   InterPro; IPR005122; Uracil-DNA_glycosylase-like.
DR   InterPro; IPR036895; Uracil-DNA_glycosylase-like_sf.
DR   Pfam; PF03167; UDG; 1.
DR   SMART; SM00986; UDG; 1.
DR   SUPFAM; SSF52141; SSF52141; 1.
DR   TIGRFAMs; TIGR04274; hypoxanDNAglyco; 1.
PE   1: Evidence at protein level;
KW   DNA damage; DNA repair; Hydrolase.
FT   CHAIN           1..158
FT                   /note="Hypoxanthine DNA glycosylase"
FT                   /id="PRO_0000439190"
FT   ACT_SITE        39
FT                   /evidence="ECO:0000305|PubMed:21642431"
FT   MUTAGEN         39
FT                   /note="N->A: Abolishes the glycosylase activity."
FT                   /evidence="ECO:0000269|PubMed:21642431"
FT   MUTAGEN         39
FT                   /note="N->D: Active only on the G/I substrate."
FT                   /evidence="ECO:0000269|PubMed:21642431"
FT   MUTAGEN         39
FT                   /note="N->Q: Retains significant activity on the G/I
FT                   substrate, but negligible activity on the T/I substrate."
FT                   /evidence="ECO:0000269|PubMed:21642431"
FT   MUTAGEN         74
FT                   /note="D->A,N: Retains significant activity on the G/I
FT                   substrate."
FT                   /evidence="ECO:0000269|PubMed:21642431"
FT   MUTAGEN         86
FT                   /note="D->A: Retains significant activity on the G/I
FT                   substrate."
FT                   /evidence="ECO:0000269|PubMed:21642431"
FT   MUTAGEN         113
FT                   /note="N->A: Retains significant activity on the G/I
FT                   substrate."
FT                   /evidence="ECO:0000269|PubMed:21642431"
SQ   SEQUENCE   158 AA;  18012 MW;  34F9ACA087753CCC CRC64;
     MKKQGFPPVI DENTEILILG SLPGDVSIRK HQYYGHPGND FWRLLGSIIG EDLQSINYQN
     RLEALKRNKI GLWDVFKAGK REGNEDTKIK DEEINQFSIL KDMAPNLKLV LFNGKKSGEY
     EPILRAMGYE TKILLSSSGA NRRSLKSRKS GWAEAFKR