HDHD2_HUMAN
ID HDHD2_HUMAN Reviewed; 259 AA.
AC Q9H0R4; A8K7T3; Q96NV4;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Haloacid dehalogenase-like hydrolase domain-containing protein 2;
GN Name=HDHD2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=11230166; DOI=10.1101/gr.gr1547r;
RA Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S.,
RA Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J.,
RA Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W.,
RA Ottenwaelder B., Obermaier B., Tampe J., Heubner D., Wambutt R., Korn B.,
RA Klein M., Poustka A.;
RT "Towards a catalog of human genes and proteins: sequencing and analysis of
RT 500 novel complete protein coding human cDNAs.";
RL Genome Res. 11:422-435(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Neuroblastoma, and Synovium;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Brain, Lung carcinoma, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP PROTEIN SEQUENCE OF 153-173, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Fetal brain cortex;
RA Lubec G., Chen W.-Q., Sun Y.;
RL Submitted (DEC-2008) to UniProtKB.
RN [7]
RP X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
RG Structural genomics consortium (SGC);
RT "The crystal structure of human haloacid dehalogenase-like hydrolase domain
RT containing 2 (HDHD2).";
RL Submitted (JUL-2009) to the PDB data bank.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000250};
CC -!- INTERACTION:
CC Q9H0R4; P50222: MEOX2; NbExp=3; IntAct=EBI-10304657, EBI-748397;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9H0R4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9H0R4-2; Sequence=VSP_025373;
CC -!- SIMILARITY: Belongs to the HAD-like hydrolase superfamily.
CC {ECO:0000305}.
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DR EMBL; AL136681; CAB66616.1; -; mRNA.
DR EMBL; AK054590; BAB70768.1; -; mRNA.
DR EMBL; AK292098; BAF84787.1; -; mRNA.
DR EMBL; CR533487; CAG38518.1; -; mRNA.
DR EMBL; CH471096; EAW62905.1; -; Genomic_DNA.
DR EMBL; BC011894; AAH11894.1; -; mRNA.
DR EMBL; BC033031; AAH33031.1; -; mRNA.
DR EMBL; BC038100; AAH38100.1; -; mRNA.
DR CCDS; CCDS32829.1; -. [Q9H0R4-1]
DR RefSeq; NP_001305694.1; NM_001318765.1. [Q9H0R4-2]
DR RefSeq; NP_115500.1; NM_032124.4. [Q9H0R4-1]
DR RefSeq; XP_006722620.1; XM_006722557.2. [Q9H0R4-2]
DR RefSeq; XP_011524529.1; XM_011526227.2. [Q9H0R4-1]
DR RefSeq; XP_011524530.1; XM_011526228.2. [Q9H0R4-1]
DR RefSeq; XP_016881528.1; XM_017026039.1. [Q9H0R4-1]
DR PDB; 3HLT; X-ray; 2.30 A; A/C=1-259.
DR PDBsum; 3HLT; -.
DR AlphaFoldDB; Q9H0R4; -.
DR SMR; Q9H0R4; -.
DR BioGRID; 123859; 13.
DR IntAct; Q9H0R4; 6.
DR STRING; 9606.ENSP00000300605; -.
DR DEPOD; HDHD2; -.
DR GlyGen; Q9H0R4; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q9H0R4; -.
DR PhosphoSitePlus; Q9H0R4; -.
DR BioMuta; HDHD2; -.
DR DMDM; 74733528; -.
DR OGP; Q9H0R4; -.
DR REPRODUCTION-2DPAGE; IPI00783874; -.
DR EPD; Q9H0R4; -.
DR jPOST; Q9H0R4; -.
DR MassIVE; Q9H0R4; -.
DR MaxQB; Q9H0R4; -.
DR PaxDb; Q9H0R4; -.
DR PeptideAtlas; Q9H0R4; -.
DR PRIDE; Q9H0R4; -.
DR ProteomicsDB; 80316; -. [Q9H0R4-1]
DR ProteomicsDB; 80317; -. [Q9H0R4-2]
DR Antibodypedia; 22539; 256 antibodies from 20 providers.
DR DNASU; 84064; -.
DR Ensembl; ENST00000300605.11; ENSP00000300605.4; ENSG00000167220.12. [Q9H0R4-1]
DR GeneID; 84064; -.
DR KEGG; hsa:84064; -.
DR MANE-Select; ENST00000300605.11; ENSP00000300605.4; NM_032124.5; NP_115500.1.
DR CTD; 84064; -.
DR DisGeNET; 84064; -.
DR GeneCards; HDHD2; -.
DR HGNC; HGNC:25364; HDHD2.
DR HPA; ENSG00000167220; Low tissue specificity.
DR neXtProt; NX_Q9H0R4; -.
DR OpenTargets; ENSG00000167220; -.
DR PharmGKB; PA134952019; -.
DR VEuPathDB; HostDB:ENSG00000167220; -.
DR eggNOG; KOG3040; Eukaryota.
DR GeneTree; ENSGT00940000155805; -.
DR HOGENOM; CLU_043473_4_0_1; -.
DR InParanoid; Q9H0R4; -.
DR OMA; PNPYFFE; -.
DR PhylomeDB; Q9H0R4; -.
DR TreeFam; TF314344; -.
DR PathwayCommons; Q9H0R4; -.
DR SignaLink; Q9H0R4; -.
DR BioGRID-ORCS; 84064; 8 hits in 1082 CRISPR screens.
DR ChiTaRS; HDHD2; human.
DR EvolutionaryTrace; Q9H0R4; -.
DR GeneWiki; HDHD2; -.
DR GenomeRNAi; 84064; -.
DR Pharos; Q9H0R4; Tdark.
DR PRO; PR:Q9H0R4; -.
DR Proteomes; UP000005640; Chromosome 18.
DR RNAct; Q9H0R4; protein.
DR Bgee; ENSG00000167220; Expressed in substantia nigra and 103 other tissues.
DR ExpressionAtlas; Q9H0R4; baseline and differential.
DR Genevisible; Q9H0R4; HS.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0019899; F:enzyme binding; IPI:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016791; F:phosphatase activity; IBA:GO_Central.
DR GO; GO:0016311; P:dephosphorylation; IEA:InterPro.
DR CDD; cd07509; HAD_PPase; 1.
DR Gene3D; 3.40.50.1000; -; 2.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR006357; HAD-SF_hydro_IIA.
DR InterPro; IPR023214; HAD_sf.
DR InterPro; IPR006355; LHPP/HDHD2.
DR Pfam; PF13344; Hydrolase_6; 1.
DR SUPFAM; SSF56784; SSF56784; 1.
DR TIGRFAMs; TIGR01460; HAD-SF-IIA; 1.
DR TIGRFAMs; TIGR01458; HAD-SF-IIA-hyp3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Coiled coil; Direct protein sequencing;
KW Magnesium; Metal-binding; Reference proteome.
FT CHAIN 1..259
FT /note="Haloacid dehalogenase-like hydrolase domain-
FT containing protein 2"
FT /id="PRO_0000287203"
FT COILED 47..71
FT /evidence="ECO:0000255"
FT BINDING 13..15
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 13
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 15
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 46..47
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 179
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 204
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT MOD_RES 50
FT /note="N6-succinyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q3UGR5"
FT VAR_SEQ 1..90
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_025373"
FT VARIANT 85
FT /note="R -> Q (in dbSNP:rs7230131)"
FT /id="VAR_032289"
FT STRAND 9..13
FT /evidence="ECO:0007829|PDB:3HLT"
FT TURN 15..17
FT /evidence="ECO:0007829|PDB:3HLT"
FT STRAND 18..24
FT /evidence="ECO:0007829|PDB:3HLT"
FT HELIX 28..37
FT /evidence="ECO:0007829|PDB:3HLT"
FT STRAND 41..46
FT /evidence="ECO:0007829|PDB:3HLT"
FT HELIX 53..62
FT /evidence="ECO:0007829|PDB:3HLT"
FT HELIX 69..71
FT /evidence="ECO:0007829|PDB:3HLT"
FT STRAND 72..74
FT /evidence="ECO:0007829|PDB:3HLT"
FT HELIX 75..86
FT /evidence="ECO:0007829|PDB:3HLT"
FT STRAND 90..94
FT /evidence="ECO:0007829|PDB:3HLT"
FT HELIX 96..102
FT /evidence="ECO:0007829|PDB:3HLT"
FT STRAND 112..115
FT /evidence="ECO:0007829|PDB:3HLT"
FT TURN 119..121
FT /evidence="ECO:0007829|PDB:3HLT"
FT HELIX 124..135
FT /evidence="ECO:0007829|PDB:3HLT"
FT STRAND 140..143
FT /evidence="ECO:0007829|PDB:3HLT"
FT STRAND 148..151
FT /evidence="ECO:0007829|PDB:3HLT"
FT STRAND 154..157
FT /evidence="ECO:0007829|PDB:3HLT"
FT HELIX 160..170
FT /evidence="ECO:0007829|PDB:3HLT"
FT HELIX 182..189
FT /evidence="ECO:0007829|PDB:3HLT"
FT TURN 190..193
FT /evidence="ECO:0007829|PDB:3HLT"
FT HELIX 196..198
FT /evidence="ECO:0007829|PDB:3HLT"
FT STRAND 199..204
FT /evidence="ECO:0007829|PDB:3HLT"
FT TURN 206..209
FT /evidence="ECO:0007829|PDB:3HLT"
FT HELIX 210..214
FT /evidence="ECO:0007829|PDB:3HLT"
FT TURN 215..217
FT /evidence="ECO:0007829|PDB:3HLT"
FT STRAND 219..225
FT /evidence="ECO:0007829|PDB:3HLT"
FT HELIX 232..235
FT /evidence="ECO:0007829|PDB:3HLT"
FT STRAND 236..238
FT /evidence="ECO:0007829|PDB:3HLT"
FT STRAND 241..246
FT /evidence="ECO:0007829|PDB:3HLT"
FT HELIX 247..257
FT /evidence="ECO:0007829|PDB:3HLT"
SQ SEQUENCE 259 AA; 28536 MW; 1367F65369CE1CBA CRC64;
MAACRALKAV LVDLSGTLHI EDAAVPGAQE ALKRLRGASV IIRFVTNTTK ESKQDLLERL
RKLEFDISED EIFTSLTAAR SLLERKQVRP MLLVDDRALP DFKGIQTSDP NAVVMGLAPE
HFHYQILNQA FRLLLDGAPL IAIHKARYYK RKDGLALGPG PFVTALEYAT DTKATVVGKP
EKTFFLEALR GTGCEPEEAV MIGDDCRDDV GGAQDVGMLG ILVKTGKYRA SDEEKINPPP
YLTCESFPHA VDHILQHLL
