HDM_DROME
ID HDM_DROME Reviewed; 486 AA.
AC Q9W3M9; D3PK97;
DT 22-JAN-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Protein hold'em;
GN Name=hdm; ORFNames=CG15329;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.;
RL Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INTERACTION WITH MEI-9 AND ERCC1.
RX PubMed=18957705; DOI=10.1534/genetics.108.093104;
RA Joyce E.F., Tanneti S.N., McKim K.S.;
RT "Drosophila hold'em is required for a subset of meiotic crossovers and
RT interacts with the dna repair endonuclease complex subunits MEI-9 and
RT ERCC1.";
RL Genetics 181:335-340(2009).
CC -!- FUNCTION: Single-stranded DNA-binding protein required for meiosis. May
CC be involved in the resolution of recombination intermediates into
CC crossovers in the meiotic recombination pathway.
CC {ECO:0000269|PubMed:18957705}.
CC -!- SUBUNIT: Interacts with mei-9 and Ercc1. {ECO:0000269|PubMed:18957705}.
CC -!- DISRUPTION PHENOTYPE: Reduced meiotic crossover formation and
CC sensitivity to the DNA-damaging agent methyl methanesulfonate (MMS).
CC {ECO:0000269|PubMed:18957705}.
CC -!- SIMILARITY: Belongs to the MEIOB family. {ECO:0000305}.
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DR EMBL; AE014298; AAF46293.1; -; Genomic_DNA.
DR EMBL; BT122053; ADD31633.1; -; mRNA.
DR RefSeq; NP_572422.1; NM_132194.1.
DR AlphaFoldDB; Q9W3M9; -.
DR SMR; Q9W3M9; -.
DR BioGRID; 58179; 6.
DR STRING; 7227.FBpp0071085; -.
DR PaxDb; Q9W3M9; -.
DR EnsemblMetazoa; FBtr0071133; FBpp0071085; FBgn0029977.
DR GeneID; 31705; -.
DR KEGG; dme:Dmel_CG15329; -.
DR UCSC; CG15329-RA; d. melanogaster.
DR CTD; 31705; -.
DR FlyBase; FBgn0029977; hdm.
DR VEuPathDB; VectorBase:FBgn0029977; -.
DR eggNOG; KOG0851; Eukaryota.
DR GeneTree; ENSGT00390000001723; -.
DR HOGENOM; CLU_042457_1_0_1; -.
DR InParanoid; Q9W3M9; -.
DR OMA; SCTLIYE; -.
DR OrthoDB; 615895at2759; -.
DR PhylomeDB; Q9W3M9; -.
DR BioGRID-ORCS; 31705; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 31705; -.
DR PRO; PR:Q9W3M9; -.
DR Proteomes; UP000000803; Chromosome X.
DR Genevisible; Q9W3M9; DM.
DR GO; GO:0032991; C:protein-containing complex; IPI:FlyBase.
DR GO; GO:0008310; F:single-stranded DNA 3'-5' exodeoxyribonuclease activity; IBA:GO_Central.
DR GO; GO:0003697; F:single-stranded DNA binding; ISM:FlyBase.
DR GO; GO:0006302; P:double-strand break repair; IMP:FlyBase.
DR GO; GO:0030716; P:oocyte fate determination; IMP:FlyBase.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:FlyBase.
DR GO; GO:0000712; P:resolution of meiotic recombination intermediates; IMP:FlyBase.
DR Gene3D; 2.40.50.140; -; 1.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR SUPFAM; SSF50249; SSF50249; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Meiosis; Reference proteome.
FT CHAIN 1..486
FT /note="Protein hold'em"
FT /id="PRO_0000425212"
FT DNA_BIND 166..285
FT /note="OB"
FT CONFLICT 23
FT /note="V -> F (in Ref. 3; ADD31633)"
FT /evidence="ECO:0000305"
FT CONFLICT 91
FT /note="M -> I (in Ref. 3; ADD31633)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 486 AA; 54674 MW; 05D09665A54990AF CRC64;
MARRIKFQRL AEMRPTMTRF STVALIVSKS SPNIFYDKMS GTERGVLSLT IRDSPNHLTN
CKCWGQRDCV DEYAAMLQIG HVVDIVGAKV MSIPFAAPGE QRYQPQATVS CALVVNEGSG
YVVRHDNDDF GQITILQQLL HQPIRPLGAV LKLADVRSGL GFPDKIITTN VNLLVVVAAV
RPVRQIKRKL QGPLSEVQEL LQCLEVIVID ASYPEGMLLS VWQPDWIQRA QQWQPRRTVL
HLIDVRVSYS NFHRSLVLSH SNCTLICENP QAAGDDCRLL LAFAATVPLT TFSGCDQAEL
DNMPAVASIQ AQMTVRQIYS RAEGELQDPS IHQFTAVLYG MVTKFDLDGL TSHVNRKCIA
CQQHIPRNLQ DCASDACQQY FSLDNDEPRS ISYFNINIHL SDQTGTLVEA RLAGHPAERI
LGLRAEDFER LAEREKSELK WRFLLKYFEV RLMIKKPVGV RNHLVIVVVD MQAIPLEKLV
ANMVVF
