ID   HDNUC_BPT4              Reviewed;         150 AA.
AC   P15075;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 2.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=Head completion nuclease {ECO:0000255|HAMAP-Rule:MF_04160};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_04160, ECO:0000269|PubMed:32056848};
DE   AltName: Full=Head completion protein Gp50;
DE   AltName: Full=Protein Gp4;
GN   Name=50; Synonyms=4, 65;
OS   Enterobacteria phage T4 (Bacteriophage T4).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus.
OX   NCBI_TaxID=10665;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2740234; DOI=10.1093/nar/17.11.4392;
RA   Koch T., Lamm N., Rueger W.;
RT   "Sequencing, cloning and overexpression of genes of bacteriophage T4
RT   between map positions 74.325 and 77.184.";
RL   Nucleic Acids Res. 17:4392-4392(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12626685; DOI=10.1128/mmbr.67.1.86-156.2003;
RA   Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.;
RT   "Bacteriophage T4 genome.";
RL   Microbiol. Mol. Biol. Rev. 67:86-156(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 30-150.
RX   PubMed=2644202; DOI=10.1128/jb.171.1.488-497.1989;
RA   Lipinska B., Rao A.S.M.K., Bolten B.M., Balakrishnan R., Goldberg E.B.;
RT   "Cloning and identification of bacteriophage T4 gene 2 product gp2 and
RT   action of gp2 on infecting DNA in vivo.";
RL   J. Bacteriol. 171:488-497(1989).
RN   [4]
RP   FUNCTION, AND MUTAGENESIS OF GLU-29.
RX   PubMed=32056848; DOI=10.1016/j.virol.2020.01.008;
RA   Benler S., Hung S.H., Vander Griend J.A., Peters G.A., Rohwer F.,
RA   Segall A.M.;
RT   "Gp4 is a nuclease required for morphogenesis of T4-like bacteriophages.";
RL   Virology 543:7-12(2020).
CC   -!- FUNCTION: During phage morphogenesis, plays an essential role in the
CC       head-tail joining step (PubMed:32056848). The associated nuclease
CC       activity is essential for morphogenesis, possibly by cleaving packaged
CC       DNA to enable the joining of heads to tails (PubMed:32056848). Displays
CC       both exo- and endonuclease activity (PubMed:32056848).
CC       {ECO:0000255|HAMAP-Rule:MF_04160, ECO:0000269|PubMed:32056848}.
CC   -!- SIMILARITY: Belongs to the Caudovirales head completion nuclease
CC       family. {ECO:0000255|HAMAP-Rule:MF_04160}.
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DR   EMBL; X14845; CAA32950.1; -; Genomic_DNA.
DR   EMBL; AF158101; AAD42481.1; -; Genomic_DNA.
DR   EMBL; M23012; AAA50417.1; ALT_SEQ; Genomic_DNA.
DR   PIR; A32254; ZTBPT9.
DR   RefSeq; NP_049755.1; NC_000866.4.
DR   GeneID; 1258590; -.
DR   KEGG; vg:1258590; -.
DR   Proteomes; UP000009087; Genome.
DR   GO; GO:0004519; F:endonuclease activity; IDA:UniProtKB.
DR   GO; GO:0004527; F:exonuclease activity; IDA:UniProtKB.
DR   HAMAP; MF_04160; NUCL_HEAD_T4; 1.
DR   InterPro; IPR046390; NUCL_HEAD_T4.
PE   1: Evidence at protein level;
KW   Endonuclease; Exonuclease; Hydrolase; Nuclease; Reference proteome.
FT   CHAIN           1..150
FT                   /note="Head completion nuclease"
FT                   /id="PRO_0000165040"
FT   ACT_SITE        29
FT                   /evidence="ECO:0000250|UniProtKB:P13988, ECO:0000255|HAMAP-
FT                   Rule:MF_04160"
FT   ACT_SITE        68
FT                   /evidence="ECO:0000250|UniProtKB:P13988, ECO:0000255|HAMAP-
FT                   Rule:MF_04160"
FT   ACT_SITE        84
FT                   /evidence="ECO:0000250|UniProtKB:P13988, ECO:0000255|HAMAP-
FT                   Rule:MF_04160"
FT   MUTAGEN         29
FT                   /note="E->C: Reduced nuclease activity."
FT                   /evidence="ECO:0000269|PubMed:32056848"
FT   CONFLICT        41
FT                   /note="E -> K (in Ref. 3; AAA50417)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        78
FT                   /note="E -> K (in Ref. 3; AAA50417)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   150 AA;  17627 MW;  4E4B485288E8FD2D CRC64;
     MAYSGKWVPK NISKYRGDPK KITYRSNWEK FFFEWLDKNP EIIAWGSETA VIPYFCNAEG
     KKRRYFMDIW MKDSSGQEFF IEIKPKKETQ PPVKPAHLTT AAKKRFMNEI YTWSVNTDKW
     KAAQSLAEKR GIKFRILTED GLRALGFKGA