ID   HDRB_METTH              Reviewed;         302 AA.
AC   O27907;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=H(2):CoB-CoM heterodisulfide,ferredoxin reductase subunit B {ECO:0000250|UniProtKB:Q50755};
DE            EC=1.8.98.5 {ECO:0000250|UniProtKB:Q50755};
DE   AltName: Full=CoB--CoM heterodisulfide reductase subunit B {ECO:0000250|UniProtKB:Q50755};
GN   Name=hdrB; OrderedLocusNames=MTH_1879;
OS   Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS   10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=187420;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA   Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA   Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA   Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA   Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA   Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA   Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA   Reeve J.N.;
RT   "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT   functional analysis and comparative genomics.";
RL   J. Bacteriol. 179:7135-7155(1997).
CC   -!- FUNCTION: Part of a complex that catalyzes the reversible reduction of
CC       CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB
CC       (coenzyme B). {ECO:0000250|UniProtKB:Q50755}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=coenzyme B + coenzyme M + 2 H(+) + 2 reduced [2Fe-2S]-
CC         [ferredoxin] = coenzyme M-coenzyme B heterodisulfide + 2 H2 + 2
CC         oxidized [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:55748, Rhea:RHEA-
CC         COMP:10000, Rhea:RHEA-COMP:10001, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:18276, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738,
CC         ChEBI:CHEBI:58319, ChEBI:CHEBI:58411, ChEBI:CHEBI:58596; EC=1.8.98.5;
CC         Evidence={ECO:0000250|UniProtKB:Q50755};
CC   -!- PATHWAY: Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide
CC       reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B
CC       heterodisulfide: step 1/1. {ECO:0000250|UniProtKB:Q50755}.
CC   -!- SUBUNIT: The heterodisulfide reductase is composed of three subunits;
CC       HdrA, HdrB and HdrC. It forms a complex with the F420-non-reducing
CC       hydrogenase (Mvh), which provides the reducing equivalents to the
CC       heterodisulfide reductase. {ECO:0000250|UniProtKB:Q50755}.
CC   -!- SIMILARITY: Belongs to the HdrB family. {ECO:0000305}.
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DR   EMBL; AE000666; AAB86345.1; -; Genomic_DNA.
DR   PIR; D69118; D69118.
DR   RefSeq; WP_010877481.1; NC_000916.1.
DR   AlphaFoldDB; O27907; -.
DR   SMR; O27907; -.
DR   IntAct; O27907; 1.
DR   STRING; 187420.MTH_1879; -.
DR   EnsemblBacteria; AAB86345; AAB86345; MTH_1879.
DR   GeneID; 1470964; -.
DR   KEGG; mth:MTH_1879; -.
DR   PATRIC; fig|187420.15.peg.1834; -.
DR   HOGENOM; CLU_052147_1_0_2; -.
DR   OMA; PGCVAQG; -.
DR   BioCyc; MetaCyc:HDRBMAUTO-MON; -.
DR   UniPathway; UPA00647; UER00700.
DR   Proteomes; UP000005223; Chromosome.
DR   GO; GO:0051912; F:CoB--CoM heterodisulfide reductase activity; IEA:InterPro.
DR   GO; GO:0015948; P:methanogenesis; IEA:UniProtKB-KW.
DR   InterPro; IPR017678; CoB/CoM_hetero-S_Rdtase_bsu.
DR   InterPro; IPR004017; Cys_rich_dom.
DR   Pfam; PF02754; CCG; 2.
DR   TIGRFAMs; TIGR03288; CoB_CoM_SS_B; 1.
PE   3: Inferred from homology;
KW   Methanogenesis; Oxidoreductase; Reference proteome.
FT   CHAIN           1..302
FT                   /note="H(2):CoB-CoM heterodisulfide,ferredoxin reductase
FT                   subunit B"
FT                   /id="PRO_0000150069"
SQ   SEQUENCE   302 AA;  33513 MW;  1BE40A77DE7DDFDC CRC64;
     MEIAYFLGCI MNNRYPGIEK ATRVLFDKLG IELKDMEGAF CCPAPGVFGS FDKTTWAAIA
     ARNITIAEEM GSDVMTECNG CFGSLFEANH LLKEDEEMRA KINEILKEAG REYKGEINVR
     HLAEILYNDV GLDKLSEVVE KPLNLNVAVH YGCHFLKPSE EINIDNPERP TILDELVEVT
     GAKSVDYKDK MMCCGAGGGV RSRDLDVALD FTMEKLRNMK EAGVDAIVNV CPFCHLQFDV
     GQMEIKDKFG EEFDIPVLHL AQLLGLAMGL PKEDLVVDAH QVCVDECLEK LEELDRLAPG
     SG