ID   ANF_LITCT               Reviewed;         145 AA.
AC   P18909;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 2.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Natriuretic peptides A;
DE   Contains:
DE     RecName: Full=Atrial natriuretic factor;
DE              Short=ANF;
DE     AltName: Full=Atrial natriuretic peptide;
DE              Short=ANP;
DE   Contains:
DE     RecName: Full=ANP-24;
DE   Flags: Precursor;
OS   Lithobates catesbeianus (American bullfrog) (Rana catesbeiana).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Lithobates.
OX   NCBI_TaxID=8400;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Kojima M.;
RL   Submitted (JUL-1992) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   PROTEIN SEQUENCE OF 122-145.
RC   TISSUE=Heart;
RX   PubMed=2972279; DOI=10.1016/s0006-291x(88)81288-9;
RA   Sakata J., Kangawa K., Matsuo H.;
RT   "Identification of new atrial natriuretic peptides in frog heart.";
RL   Biochem. Biophys. Res. Commun. 155:1338-1345(1988).
CC   -!- FUNCTION: Hormone playing a key role in cardiovascular homeostasis
CC       through regulation of natriuresis, diuresis, and vasodilation. Has a
CC       cGMP-stimulating activity (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- PTM: Cleaved upon secretion to produce the functional hormone.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the natriuretic peptide family. {ECO:0000305}.
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DR   EMBL; D01043; BAA00850.1; -; mRNA.
DR   PIR; JQ0947; JQ0947.
DR   AlphaFoldDB; P18909; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   GO; GO:0006182; P:cGMP biosynthetic process; ISS:UniProtKB.
DR   GO; GO:0007168; P:receptor guanylyl cyclase signaling pathway; ISS:UniProtKB.
DR   InterPro; IPR000663; Natr_peptide.
DR   InterPro; IPR030480; Natr_peptide_CS.
DR   InterPro; IPR002407; Natriuretic_peptide_atrial.
DR   Pfam; PF00212; ANP; 1.
DR   PRINTS; PR00711; ANATPEPTIDE.
DR   PRINTS; PR00710; NATPEPTIDES.
DR   SMART; SM00183; NAT_PEP; 1.
DR   PROSITE; PS00263; NATRIURETIC_PEPTIDE; 1.
PE   1: Evidence at protein level;
KW   Cleavage on pair of basic residues; Direct protein sequencing;
KW   Disulfide bond; Hormone; Secreted; Signal; Vasoactive.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   PROPEP          24..117
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000001515"
FT   PEPTIDE         118..145
FT                   /note="Atrial natriuretic factor"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000391788"
FT   PEPTIDE         122..145
FT                   /note="ANP-24"
FT                   /id="PRO_0000001516"
FT   SITE            117..118
FT                   /note="Cleavage"
FT                   /evidence="ECO:0000250"
FT   DISULFID        125..141
SQ   SEQUENCE   145 AA;  15934 MW;  01EB3B9779529405 CRC64;
     MGTSFVGYLT FVLLLLALTK VRGGPAYNSP LSSDLSDLKG LLERLEDRLP VEEVETPVQD
     IFAPNYDSAD SSNSAPSLTV EAARPGADMM YNRGSWTQQE KSSPLRNKLR ELLNAPRSMR
     RSSDCFGSRI DRIGAQSGMG CGRRF