HE71A_XENLA
ID HE71A_XENLA Reviewed; 180 AA.
AC Q8UW74; Q8UW73;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 25-MAY-2022, entry version 79.
DE RecName: Full=Transcription factor HES-7.1-A;
DE AltName: Full=HES-related protein 1-A;
DE Short=XHR1-A {ECO:0000312|EMBL:BAB78540.1};
DE AltName: Full=HES-related protein 1-A';
DE Short=XHR1-A' {ECO:0000312|EMBL:BAB78541.1};
DE AltName: Full=Hairy and enhancer of split 7.1-A;
GN Name=hes7.1-a;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1] {ECO:0000305, ECO:0000312|EMBL:BAB78540.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND MUTAGENESIS
RP OF GLU-22; LYS-23 AND ARG-26.
RC TISSUE=Gastrula {ECO:0000269|PubMed:11731236};
RX PubMed=11731236; DOI=10.1016/s0925-4773(01)00528-7;
RA Shinga J., Itoh M., Shiokawa K., Taira S., Taira M.;
RT "Early patterning of the prospective midbrain-hindbrain boundary by the
RT HES-related gene XHR1 in Xenopus embryos.";
RL Mech. Dev. 109:225-239(2001).
RN [2] {ECO:0000312|EMBL:AAI70054.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Gastrula {ECO:0000312|EMBL:AAI70054.1};
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305}
RP FUNCTION, AND LACK OF INDUCTION BY NOTCH SIGNALING.
RX PubMed=15935340; DOI=10.1016/j.ydbio.2005.04.020;
RA Takada H., Hattori D., Kitayama A., Ueno N., Taira M.;
RT "Identification of target genes for the Xenopus Hes-related protein XHR1, a
RT prepattern factor specifying the midbrain-hindbrain boundary.";
RL Dev. Biol. 283:253-267(2005).
CC -!- FUNCTION: Transcriptional repressor. Represses transcription from both
CC N box- and E box-containing promoters (By similarity). Demarcates the
CC prospective midbrain-hindbrain boundary (MHB) region in the
CC neuroectoderm in early gastrulae embryos by repressing transcription of
CC a number of target genes. {ECO:0000250|UniProtKB:Q8BKT2,
CC ECO:0000269|PubMed:11731236, ECO:0000269|PubMed:15935340}.
CC -!- SUBUNIT: Transcription repression requires formation of a complex with
CC a corepressor protein of the Groucho/TLE family.
CC {ECO:0000250|UniProtKB:P14003}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8BKT2,
CC ECO:0000255|PROSITE-ProRule:PRU00380, ECO:0000255|PROSITE-
CC ProRule:PRU00981}.
CC -!- TISSUE SPECIFICITY: Expressed in the presumptive midbrain-hindbrain
CC boundary (MHB) as early as the early gastrula stage (stage 10.5).
CC Expression in the MHB continues through to tailbud stage. Also
CC transiently expressed in the eye anlage at late neurula stage.
CC {ECO:0000269|PubMed:11731236}.
CC -!- INDUCTION: Not induced by Notch-signaling.
CC {ECO:0000269|PubMed:15935340}.
CC -!- DOMAIN: Has a particular type of basic domain which includes a helix-
CC interrupting proline.
CC -!- DOMAIN: The C-terminal WRPW motif is a transcriptional repression motif
CC which is necessary for interaction with Groucho/TLE family members,
CC transcriptional corepressors recruited to specific target DNA by Hairy-
CC related proteins. {ECO:0000250}.
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DR EMBL; AB071432; BAB78540.1; -; mRNA.
DR EMBL; AB071433; BAB78541.1; -; mRNA.
DR EMBL; BC170054; AAI70054.1; -; mRNA.
DR AlphaFoldDB; Q8UW74; -.
DR SMR; Q8UW74; -.
DR ELM; Q8UW74; -.
DR OMA; MEAHRKL; -.
DR Proteomes; UP000186698; Genome assembly.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0030917; P:midbrain-hindbrain boundary development; IMP:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0007219; P:Notch signaling pathway; IEA:InterPro.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR032644; HES-7.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR003650; Orange_dom.
DR PANTHER; PTHR10985:SF84; PTHR10985:SF84; 1.
DR Pfam; PF07527; Hairy_orange; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
DR PROSITE; PS51054; ORANGE; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..180
FT /note="Transcription factor HES-7.1-A"
FT /id="PRO_0000370215"
FT DOMAIN 13..70
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT DOMAIN 84..116
FT /note="Orange"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00380"
FT MOTIF 176..179
FT /note="WRPW motif"
FT /evidence="ECO:0000255"
FT MUTAGEN 22
FT /note="E->A: Reduces MHB gene expression in a dominant
FT negative manner; when associated with A-23 and A-26."
FT /evidence="ECO:0000269|PubMed:11731236"
FT MUTAGEN 23
FT /note="K->A: Reduces MHB gene expression in a dominant
FT negative manner; when associated with A-22 and A-26."
FT /evidence="ECO:0000269|PubMed:11731236"
FT MUTAGEN 26
FT /note="R->A: Reduces MHB gene expression in a dominant
FT negative manner; when associated with A-22 and A-23."
FT /evidence="ECO:0000269|PubMed:11731236"
FT CONFLICT 148
FT /note="P -> L (in Ref. 1; BAB78541)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 180 AA; 20826 MW; 540238FF0BF76801 CRC64;
MKGTSEVKPM ETHRKLLKPL VEKRRRERIN NSLEKLRIFL FQTLKSEKLK NPKVEKAEIL
ECTVQFLQSR KLLPLDREAV DKEYQSGFQH CLETTLHFMN SKPDMNGVTK ELLSHQMSSC
KSPSDAWSPN CAPLTKHVPS LSYQDSAPHL VSNSISISPT KTLVDSHFTY QSFKTWRPWV
