HE71B_XENLA
ID HE71B_XENLA Reviewed; 180 AA.
AC Q8UW72;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 85.
DE RecName: Full=Transcription factor HES-7.1-B;
DE AltName: Full=HES-related protein 1-B;
DE Short=XHR1-B {ECO:0000312|EMBL:BAB78542.1};
DE AltName: Full=Hairy and enhancer of split 7.1-B;
GN Name=hes7.1-b;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1] {ECO:0000305, ECO:0000312|EMBL:BAB78542.1}
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=11731236; DOI=10.1016/s0925-4773(01)00528-7;
RA Shinga J., Itoh M., Shiokawa K., Taira S., Taira M.;
RT "Early patterning of the prospective midbrain-hindbrain boundary by the
RT HES-related gene XHR1 in Xenopus embryos.";
RL Mech. Dev. 109:225-239(2001).
RN [2] {ECO:0000305}
RP FUNCTION, AND LACK OF INDUCTION BY NOTCH SIGNALING.
RX PubMed=15935340; DOI=10.1016/j.ydbio.2005.04.020;
RA Takada H., Hattori D., Kitayama A., Ueno N., Taira M.;
RT "Identification of target genes for the Xenopus Hes-related protein XHR1, a
RT prepattern factor specifying the midbrain-hindbrain boundary.";
RL Dev. Biol. 283:253-267(2005).
CC -!- FUNCTION: Transcriptional repressor. Represses transcription from both
CC N box- and E box-containing promoters (By similarity). Demarcates the
CC prospective midbrain-hindbrain boundary (MHB) region in the
CC neuroectoderm in early gastrulae embryos by repressing transcription of
CC a number of target genes. {ECO:0000250|UniProtKB:Q8BKT2,
CC ECO:0000250|UniProtKB:Q8UW74, ECO:0000269|PubMed:15935340}.
CC -!- SUBUNIT: Transcription repression requires formation of a complex with
CC a corepressor protein of the Groucho/TLE family.
CC {ECO:0000250|UniProtKB:P14003}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8BKT2,
CC ECO:0000255|PROSITE-ProRule:PRU00380, ECO:0000255|PROSITE-
CC ProRule:PRU00981}.
CC -!- TISSUE SPECIFICITY: Expressed in the presumptive midbrain-hindbrain
CC boundary (MHB) as early as the early gastrula stage (stage 10.5).
CC Expression in the MHB continues through to tailbud stage. Also
CC transiently expressed in the eye anlage at late neurula stage.
CC {ECO:0000269|PubMed:11731236}.
CC -!- INDUCTION: Not induced by Notch-signaling.
CC {ECO:0000269|PubMed:15935340}.
CC -!- DOMAIN: Has a particular type of basic domain which includes a helix-
CC interrupting proline.
CC -!- DOMAIN: The C-terminal WRPW motif is a transcriptional repression
CC domain necessary for the interaction with Groucho/TLE family members,
CC transcriptional corepressors recruited to specific target DNA by Hairy-
CC related proteins. {ECO:0000250|UniProtKB:P14003}.
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DR EMBL; AB071434; BAB78542.1; -; mRNA.
DR RefSeq; NP_001082175.1; NM_001088706.1.
DR AlphaFoldDB; Q8UW72; -.
DR SMR; Q8UW72; -.
DR ELM; Q8UW72; -.
DR PRIDE; Q8UW72; -.
DR GeneID; 398267; -.
DR KEGG; xla:398267; -.
DR CTD; 398267; -.
DR Xenbase; XB-GENE-876475; hes7.1.L.
DR OrthoDB; 1172143at2759; -.
DR Proteomes; UP000186698; Chromosome 3L.
DR Bgee; 398267; Expressed in neurula embryo and 2 other tissues.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0030917; P:midbrain-hindbrain boundary development; ISS:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0007219; P:Notch signaling pathway; IEA:InterPro.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR032644; HES-7.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR003650; Orange_dom.
DR PANTHER; PTHR10985:SF84; PTHR10985:SF84; 1.
DR Pfam; PF07527; Hairy_orange; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
DR PROSITE; PS51054; ORANGE; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..180
FT /note="Transcription factor HES-7.1-B"
FT /id="PRO_0000370216"
FT DOMAIN 13..70
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT DOMAIN 84..116
FT /note="Orange"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00380"
FT MOTIF 176..179
FT /note="WRPW motif"
FT /evidence="ECO:0000255"
SQ SEQUENCE 180 AA; 20807 MW; 52DF9D4AA0280A01 CRC64;
MKGTSEVKPM ETHRKLLKPL VEKRRRERIN NSLEKLRIFL SQTLKSEKLK NPKVEKAEIL
ECTVQFLQSR KLLPLDREAV DKEYQSGFQH CLETTLHFMN SKPDMNGVTK ELLSHQMSSC
KPPSDAWSPT CAPLTKHVPS LSYQDSTPHL VSNSISISPT KTLVDSHFTY QTFKTWRPWV
