HEAT3_HUMAN
ID HEAT3_HUMAN Reviewed; 680 AA.
AC Q7Z4Q2; A8K1N4; Q8N525; Q8WV56; Q96CC9; Q9NWN7;
DT 15-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2004, sequence version 2.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=HEAT repeat-containing protein 3;
GN Name=HEATR3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Brain;
RA Wu Q., Guo J.H., Yu L.;
RL Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Hepatoma, and Hippocampus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Pancreas, Skin, and Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-340, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q7Z4Q2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q7Z4Q2-2; Sequence=VSP_009640;
CC Name=3;
CC IsoId=Q7Z4Q2-3; Sequence=VSP_009640, VSP_009641, VSP_009642;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH14415.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF462442; AAP97710.1; -; mRNA.
DR EMBL; AK000725; BAA91342.1; -; mRNA.
DR EMBL; AK289949; BAF82638.1; -; mRNA.
DR EMBL; CH471092; EAW82743.1; -; Genomic_DNA.
DR EMBL; BC014415; AAH14415.1; ALT_INIT; mRNA.
DR EMBL; BC018730; AAH18730.2; -; mRNA.
DR EMBL; BC033077; AAH33077.1; -; mRNA.
DR CCDS; CCDS10739.1; -. [Q7Z4Q2-1]
DR RefSeq; NP_891552.1; NM_182922.3. [Q7Z4Q2-1]
DR AlphaFoldDB; Q7Z4Q2; -.
DR SMR; Q7Z4Q2; -.
DR BioGRID; 120357; 124.
DR IntAct; Q7Z4Q2; 56.
DR MINT; Q7Z4Q2; -.
DR STRING; 9606.ENSP00000299192; -.
DR GlyGen; Q7Z4Q2; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q7Z4Q2; -.
DR MetOSite; Q7Z4Q2; -.
DR PhosphoSitePlus; Q7Z4Q2; -.
DR BioMuta; HEATR3; -.
DR DMDM; 45477280; -.
DR EPD; Q7Z4Q2; -.
DR jPOST; Q7Z4Q2; -.
DR MassIVE; Q7Z4Q2; -.
DR MaxQB; Q7Z4Q2; -.
DR PaxDb; Q7Z4Q2; -.
DR PeptideAtlas; Q7Z4Q2; -.
DR PRIDE; Q7Z4Q2; -.
DR ProteomicsDB; 69221; -. [Q7Z4Q2-1]
DR ProteomicsDB; 69222; -. [Q7Z4Q2-2]
DR ProteomicsDB; 69223; -. [Q7Z4Q2-3]
DR Antibodypedia; 49437; 63 antibodies from 12 providers.
DR DNASU; 55027; -.
DR Ensembl; ENST00000299192.8; ENSP00000299192.7; ENSG00000155393.14. [Q7Z4Q2-1]
DR GeneID; 55027; -.
DR KEGG; hsa:55027; -.
DR MANE-Select; ENST00000299192.8; ENSP00000299192.7; NM_182922.4; NP_891552.1.
DR UCSC; uc002efw.4; human. [Q7Z4Q2-1]
DR CTD; 55027; -.
DR DisGeNET; 55027; -.
DR GeneCards; HEATR3; -.
DR HGNC; HGNC:26087; HEATR3.
DR HPA; ENSG00000155393; Low tissue specificity.
DR MIM; 614951; gene.
DR neXtProt; NX_Q7Z4Q2; -.
DR OpenTargets; ENSG00000155393; -.
DR PharmGKB; PA144596426; -.
DR VEuPathDB; HostDB:ENSG00000155393; -.
DR eggNOG; ENOG502QWR9; Eukaryota.
DR GeneTree; ENSGT00390000012529; -.
DR HOGENOM; CLU_028600_0_0_1; -.
DR InParanoid; Q7Z4Q2; -.
DR OMA; YRKFGLE; -.
DR OrthoDB; 475746at2759; -.
DR PhylomeDB; Q7Z4Q2; -.
DR TreeFam; TF324159; -.
DR PathwayCommons; Q7Z4Q2; -.
DR SignaLink; Q7Z4Q2; -.
DR BioGRID-ORCS; 55027; 198 hits in 1089 CRISPR screens.
DR ChiTaRS; HEATR3; human.
DR GenomeRNAi; 55027; -.
DR Pharos; Q7Z4Q2; Tbio.
DR PRO; PR:Q7Z4Q2; -.
DR Proteomes; UP000005640; Chromosome 16.
DR RNAct; Q7Z4Q2; protein.
DR Bgee; ENSG00000155393; Expressed in monocyte and 169 other tissues.
DR Genevisible; Q7Z4Q2; HS.
DR GO; GO:0051082; F:unfolded protein binding; IBA:GO_Central.
DR GO; GO:0006606; P:protein import into nucleus; IBA:GO_Central.
DR GO; GO:0042273; P:ribosomal large subunit biogenesis; IBA:GO_Central.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..680
FT /note="HEAT repeat-containing protein 3"
FT /id="PRO_0000050821"
FT REPEAT 38..69
FT /note="HEAT 1"
FT REPEAT 74..110
FT /note="HEAT 2"
FT REGION 1..39
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 15
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT MOD_RES 144
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8BQM4"
FT MOD_RES 340
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT VAR_SEQ 1..106
FT /note="MGKSRTKRFKRPQFSPTGDCQAEAAAAANGTGGEEDDGPAAELLEKLQHPSA
FT EVRECACAGLARLVQQRPALPGLARRDAVRRLGPLLLDPSLAVRETAAGALRNL -> M
FT GPEARRTTGRRRSCWKRNP (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039, ECO:0000303|Ref.1"
FT /id="VSP_009640"
FT VAR_SEQ 378..400
FT /note="DPSDDEWEELSSSDESDAFMENS -> GLLTFTFRHFPPEPRGKLPWRFS
FT (in isoform 3)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_009641"
FT VAR_SEQ 401..680
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_009642"
FT VARIANT 302
FT /note="R -> S (in dbSNP:rs34703459)"
FT /id="VAR_033989"
FT CONFLICT 197
FT /note="F -> L (in Ref. 1; AAP97710)"
FT /evidence="ECO:0000305"
FT CONFLICT Q7Z4Q2-3:20
FT /note="P -> L (in Ref. 1; AAP97710)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 680 AA; 74583 MW; F7892F94AC5103B5 CRC64;
MGKSRTKRFK RPQFSPTGDC QAEAAAAANG TGGEEDDGPA AELLEKLQHP SAEVRECACA
GLARLVQQRP ALPGLARRDA VRRLGPLLLD PSLAVRETAA GALRNLSACG GFEVCDDMVT
KDIMTPLVAL LKECSAGLDS NEMSLQEKKD QNRNSIENIA NETVNVLWNI CECSSRAVSI
FNKEGCLEIV LKYLSRFPTN VDLAISVAYC LQTVTEDNPE LLKSFSATAL NMLESALLSP
VSSMESLLLK TLVAGTIWNL KDIIPCKSQA EIINALLKIL SEVLGMDAGE MVIQMKEAET
QRLKTAAEAE EILENTNGDD LIEDDEMEGI SHKRRVRRKT FVSDLLPPTD KELRETIALL
TAQQTALEII VNMCCNEDPS DDEWEELSSS DESDAFMENS FSECGGQLFS PLCLSHEVHT
ALTNYLIPKK IFEKTAFPNS IAVDLCSRNP TWKPLIRKMN TIQCRALFCL QSLVSLLDVE
HLGGAAALQT LAQHLSQLLF SQPDFAKHVD FLEAISSALR ALLQTMASKN ISQCMTPDQL
MTLCKAGIHS SNVGVRVNVV SILGITGSVL AKEDGTLETL KNIGCFLLEV TTKDPSLVVA
GEALDALFDV FADGKEAERA SIQIKLLSAL KEFQPVFKMK IRKEGRGNYS TDQLCVLDNV
KMNLRRFIAY QETVEKRLTS
